Strain Fitness in Escherichia coli BL21 around ECD_02252

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02249 and ECD_02250 are separated by 120 nucleotidesECD_02250 and ECD_02251 are separated by 33 nucleotidesECD_02251 and ECD_02252 overlap by 1 nucleotidesECD_02252 and ECD_02253 overlap by 1 nucleotidesECD_02253 and ECD_02254 are separated by 3 nucleotides ECD_02249: ECD_02249 - fused 5-methylaminomethyl-2-thiouridine-forming enzyme methyltransferase and FAD-dependent demodification enzyme, at 2,329,017 to 2,331,023 _02249 ECD_02250: ECD_02250 - uncharacterized protein, at 2,331,144 to 2,331,422 _02250 ECD_02251: ECD_02251 - Elongation Factor P Lys34 hydroxylase, at 2,331,456 to 2,332,004 _02251 ECD_02252: ECD_02252 - TauE/TSUP family inner membrane protein, at 2,332,004 to 2,332,813 _02252 ECD_02253: ECD_02253 - murein DD-endopeptidase, at 2,332,813 to 2,333,637 _02253 ECD_02254: ECD_02254 - chorismate synthase, at 2,333,641 to 2,334,726 _02254 Position (kb) 2332 2333Strain fitness (log2 ratio) -2 -1 0 1 2at 2331.073 kb on + strandat 2331.073 kb on + strandat 2331.074 kb on - strandat 2331.261 kb on - strand, within ECD_02250at 2331.512 kb on - strand, within ECD_02251at 2331.515 kb on - strand, within ECD_02251at 2331.572 kb on - strand, within ECD_02251at 2331.592 kb on - strand, within ECD_02251at 2331.880 kb on - strand, within ECD_02251at 2331.902 kb on - strand, within ECD_02251at 2331.939 kb on - strand, within ECD_02251at 2331.963 kb on - strandat 2332.005 kb on + strandat 2332.006 kb on - strandat 2332.006 kb on - strandat 2332.056 kb on - strandat 2332.395 kb on + strand, within ECD_02252at 2332.425 kb on - strand, within ECD_02252at 2332.447 kb on - strand, within ECD_02252at 2332.488 kb on - strand, within ECD_02252at 2332.597 kb on + strand, within ECD_02252at 2332.602 kb on + strand, within ECD_02252at 2332.603 kb on - strand, within ECD_02252at 2332.606 kb on + strand, within ECD_02252at 2332.607 kb on - strand, within ECD_02252at 2332.607 kb on - strand, within ECD_02252at 2332.789 kb on + strandat 2332.801 kb on + strandat 2332.814 kb on + strandat 2332.997 kb on - strand, within ECD_02253at 2332.997 kb on - strand, within ECD_02253at 2332.997 kb on - strand, within ECD_02253at 2333.002 kb on + strand, within ECD_02253at 2333.003 kb on - strand, within ECD_02253at 2333.003 kb on - strand, within ECD_02253at 2333.435 kb on + strand, within ECD_02253at 2333.466 kb on - strand, within ECD_02253at 2333.506 kb on - strand, within ECD_02253at 2333.515 kb on - strand, within ECD_02253at 2333.515 kb on - strand, within ECD_02253

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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2,331,073 + -0.4
2,331,073 + -0.5
2,331,074 - +0.6
2,331,261 - ECD_02250 0.42 -2.5
2,331,512 - ECD_02251 0.10 +0.0
2,331,515 - ECD_02251 0.11 +0.1
2,331,572 - ECD_02251 0.21 +0.9
2,331,592 - ECD_02251 0.25 -1.0
2,331,880 - ECD_02251 0.77 -0.8
2,331,902 - ECD_02251 0.81 -0.5
2,331,939 - ECD_02251 0.88 -0.5
2,331,963 - -0.7
2,332,005 + -0.3
2,332,006 - -1.3
2,332,006 - -2.3
2,332,056 - +0.1
2,332,395 + ECD_02252 0.48 -1.8
2,332,425 - ECD_02252 0.52 -0.1
2,332,447 - ECD_02252 0.55 -0.9
2,332,488 - ECD_02252 0.60 -2.7
2,332,597 + ECD_02252 0.73 -1.1
2,332,602 + ECD_02252 0.74 -0.1
2,332,603 - ECD_02252 0.74 -1.9
2,332,606 + ECD_02252 0.74 -2.4
2,332,607 - ECD_02252 0.74 +0.0
2,332,607 - ECD_02252 0.74 +0.6
2,332,789 + +1.0
2,332,801 + -0.4
2,332,814 + -2.2
2,332,997 - ECD_02253 0.22 -0.4
2,332,997 - ECD_02253 0.22 +1.8
2,332,997 - ECD_02253 0.22 -0.2
2,333,002 + ECD_02253 0.23 +0.1
2,333,003 - ECD_02253 0.23 -1.9
2,333,003 - ECD_02253 0.23 -0.1
2,333,435 + ECD_02253 0.75 +0.1
2,333,466 - ECD_02253 0.79 -0.3
2,333,506 - ECD_02253 0.84 -0.8
2,333,515 - ECD_02253 0.85 -0.0
2,333,515 - ECD_02253 0.85 -1.0

Or see this region's nucleotide sequence