Strain Fitness in Escherichia coli BL21 around ECD_01861

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01859 and ECD_01860 are separated by 4 nucleotidesECD_01860 and ECD_01861 overlap by 4 nucleotidesECD_01861 and ECD_01862 are separated by 126 nucleotidesECD_01862 and ECD_01863 are separated by 2 nucleotides ECD_01859: ECD_01859 - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein, at 1,919,278 to 1,921,242 _01859 ECD_01860: ECD_01860 - protein that enables flagellar motor rotation, at 1,921,247 to 1,922,173 _01860 ECD_01861: ECD_01861 - proton conductor component of flagella motor, at 1,922,170 to 1,923,057 _01861 ECD_01862: ECD_01862 - flagellar class II regulon transcriptional activator, with FlhD, at 1,923,184 to 1,923,762 _01862 ECD_01863: ECD_01863 - flagellar class II regulon transcriptional activator, with FlhC, at 1,923,765 to 1,924,115 _01863 Position (kb) 1922 1923 1924Strain fitness (log2 ratio) -1 0 1 2 3at 1921.228 kb on + strandat 1921.467 kb on - strand, within ECD_01860at 1921.550 kb on - strand, within ECD_01860at 1921.550 kb on - strand, within ECD_01860at 1921.550 kb on - strand, within ECD_01860at 1921.550 kb on - strand, within ECD_01860at 1921.661 kb on - strand, within ECD_01860at 1921.727 kb on - strand, within ECD_01860at 1921.906 kb on - strand, within ECD_01860at 1922.495 kb on - strand, within ECD_01861at 1922.529 kb on + strand, within ECD_01861at 1922.833 kb on - strand, within ECD_01861at 1922.950 kb on - strand, within ECD_01861at 1923.022 kb on - strandat 1923.043 kb on + strandat 1923.051 kb on + strandat 1923.051 kb on + strandat 1923.197 kb on - strandat 1923.468 kb on - strand, within ECD_01862

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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1,921,228 + +0.5
1,921,467 - ECD_01860 0.24 +0.3
1,921,550 - ECD_01860 0.33 +2.9
1,921,550 - ECD_01860 0.33 -0.4
1,921,550 - ECD_01860 0.33 +1.2
1,921,550 - ECD_01860 0.33 +0.3
1,921,661 - ECD_01860 0.45 +1.2
1,921,727 - ECD_01860 0.52 +0.3
1,921,906 - ECD_01860 0.71 +2.2
1,922,495 - ECD_01861 0.37 +1.2
1,922,529 + ECD_01861 0.40 -1.7
1,922,833 - ECD_01861 0.75 +0.3
1,922,950 - ECD_01861 0.88 -0.6
1,923,022 - -0.3
1,923,043 + +0.2
1,923,051 + -0.6
1,923,051 + +0.0
1,923,197 - -0.0
1,923,468 - ECD_01862 0.49 -0.3

Or see this region's nucleotide sequence