Strain Fitness in Escherichia coli BL21 around ECD_01649

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01647 and ECD_01648 are separated by 148 nucleotidesECD_01648 and ECD_01649 are separated by 12 nucleotidesECD_01649 and ECD_01650 overlap by 4 nucleotides ECD_01647: ECD_01647 - murein L,D-transpeptidase, at 1,702,990 to 1,703,994 _01647 ECD_01648: ECD_01648 - sulfur acceptor protein, at 1,704,143 to 1,704,559 _01648 ECD_01649: ECD_01649 - cysteine desulfurase, stimulated by SufE; selenocysteine lyase, PLP-dependent, at 1,704,572 to 1,705,792 _01649 ECD_01650: ECD_01650 - component of SufBCD Fe-S cluster assembly scaffold, at 1,705,789 to 1,707,060 _01650 Position (kb) 1704 1705 1706Strain fitness (log2 ratio) -1 0 1 2 3at 1703.626 kb on - strand, within ECD_01647at 1704.144 kb on + strandat 1704.640 kb on - strandat 1704.679 kb on - strandat 1704.682 kb on - strandat 1704.915 kb on + strand, within ECD_01649at 1705.084 kb on - strand, within ECD_01649at 1705.418 kb on - strand, within ECD_01649at 1705.622 kb on - strand, within ECD_01649at 1705.963 kb on - strand, within ECD_01650at 1706.122 kb on - strand, within ECD_01650at 1706.176 kb on - strand, within ECD_01650at 1706.335 kb on - strand, within ECD_01650at 1706.593 kb on - strand, within ECD_01650

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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1,703,626 - ECD_01647 0.63 +0.6
1,704,144 + +0.1
1,704,640 - +0.6
1,704,679 - -1.1
1,704,682 - +3.3
1,704,915 + ECD_01649 0.28 +2.8
1,705,084 - ECD_01649 0.42 +1.0
1,705,418 - ECD_01649 0.69 -0.1
1,705,622 - ECD_01649 0.86 -0.0
1,705,963 - ECD_01650 0.14 +0.2
1,706,122 - ECD_01650 0.26 +1.8
1,706,176 - ECD_01650 0.30 +0.5
1,706,335 - ECD_01650 0.43 +1.1
1,706,593 - ECD_01650 0.63 +0.1

Or see this region's nucleotide sequence