Strain Fitness in Escherichia coli BL21 around ECD_01274

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01273 and ECD_01274 are separated by 302 nucleotidesECD_01274 and ECD_01275 are separated by 211 nucleotides ECD_01273: ECD_01273 - putrescine importer, at 1,344,168 to 1,345,553 _01273 ECD_01274: ECD_01274 - glutamate--putrescine ligase, at 1,345,856 to 1,347,274 _01274 ECD_01275: ECD_01275 - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 1,347,486 to 1,348,250 _01275 Position (kb) 1345 1346 1347 1348Strain fitness (log2 ratio) -1 0 1 2at 1345.292 kb on + strand, within ECD_01273at 1345.454 kb on - strandat 1345.696 kb on - strandat 1345.732 kb on + strandat 1345.965 kb on - strandat 1346.203 kb on + strand, within ECD_01274at 1346.204 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.471 kb on - strand, within ECD_01274at 1346.628 kb on - strand, within ECD_01274at 1347.027 kb on - strand, within ECD_01274at 1347.206 kb on - strandat 1347.252 kb on - strandat 1347.439 kb on + strandat 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1347.718 kb on + strand, within ECD_01275at 1348.030 kb on + strand, within ECD_01275at 1348.030 kb on + strand, within ECD_01275at 1348.096 kb on + strand, within ECD_01275at 1348.141 kb on + strand, within ECD_01275at 1348.189 kb on + strandat 1348.205 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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1,345,292 + ECD_01273 0.81 -0.2
1,345,454 - +1.1
1,345,696 - -0.6
1,345,732 + +1.4
1,345,965 - +2.2
1,346,203 + ECD_01274 0.24 -0.3
1,346,204 - ECD_01274 0.25 +1.3
1,346,257 - ECD_01274 0.28 +0.1
1,346,257 - ECD_01274 0.28 +0.8
1,346,257 - ECD_01274 0.28 +0.1
1,346,257 - ECD_01274 0.28 +1.1
1,346,257 - ECD_01274 0.28 +1.1
1,346,471 - ECD_01274 0.43 +0.7
1,346,628 - ECD_01274 0.54 +1.2
1,347,027 - ECD_01274 0.83 +0.5
1,347,206 - -0.3
1,347,252 - +0.7
1,347,439 + +2.0
1,347,718 + ECD_01275 0.30 -1.0
1,347,718 + ECD_01275 0.30 +0.1
1,347,718 + ECD_01275 0.30 +1.4
1,347,718 + ECD_01275 0.30 -0.8
1,348,030 + ECD_01275 0.71 -0.3
1,348,030 + ECD_01275 0.71 +1.1
1,348,096 + ECD_01275 0.80 -1.1
1,348,141 + ECD_01275 0.86 -0.6
1,348,189 + -0.3
1,348,205 + -0.1

Or see this region's nucleotide sequence