Strain Fitness in Escherichia coli BL21 around ECD_00218

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00217 and ECD_00218 are separated by 205 nucleotidesECD_00218 and ECD_00219 overlap by 30 nucleotidesECD_00219 and ECD_00220 are separated by 155 nucleotides ECD_00217: ECD_00217 - D-sedoheptulose 7-phosphate isomerase, at 248,117 to 248,695 _00217 ECD_00218: ECD_00218 - type 2 glutamine amidotransferase family protein, at 248,901 to 249,668 _00218 ECD_00219: ECD_00219 - L,D-transpeptidase-related protein, at 249,639 to 250,379 _00219 ECD_00220: ECD_00220 - mRNA interferase toxin of toxin-antitoxin pair YafQ/DinJ, at 250,535 to 250,813 _00220 Position (kb) 248 249 250Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 247.951 kb on - strandat 247.974 kb on - strandat 248.092 kb on + strandat 248.121 kb on - strandat 248.377 kb on + strand, within ECD_00217at 248.776 kb on - strandat 248.968 kb on + strandat 248.968 kb on + strandat 248.968 kb on + strandat 248.996 kb on + strand, within ECD_00218at 248.997 kb on - strand, within ECD_00218at 249.021 kb on + strand, within ECD_00218at 249.048 kb on - strand, within ECD_00218at 249.068 kb on + strand, within ECD_00218at 249.213 kb on + strand, within ECD_00218at 249.213 kb on + strand, within ECD_00218at 249.374 kb on + strand, within ECD_00218at 249.442 kb on - strand, within ECD_00218at 249.442 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.446 kb on - strand, within ECD_00218at 249.465 kb on - strand, within ECD_00218at 249.661 kb on + strandat 249.662 kb on - strandat 249.719 kb on - strand, within ECD_00219at 249.753 kb on - strand, within ECD_00219at 249.817 kb on + strand, within ECD_00219at 249.818 kb on - strand, within ECD_00219at 249.863 kb on + strand, within ECD_00219at 249.863 kb on + strand, within ECD_00219at 249.989 kb on - strand, within ECD_00219at 250.019 kb on - strand, within ECD_00219at 250.019 kb on - strand, within ECD_00219at 250.038 kb on - strand, within ECD_00219at 250.041 kb on - strand, within ECD_00219at 250.051 kb on + strand, within ECD_00219at 250.052 kb on - strand, within ECD_00219at 250.208 kb on - strand, within ECD_00219at 250.208 kb on - strand, within ECD_00219at 250.221 kb on - strand, within ECD_00219at 250.221 kb on - strand, within ECD_00219at 250.274 kb on + strand, within ECD_00219at 250.274 kb on + strand, within ECD_00219at 250.275 kb on - strand, within ECD_00219at 250.275 kb on - strand, within ECD_00219at 250.275 kb on - strand, within ECD_00219at 250.275 kb on - strand, within ECD_00219at 250.275 kb on - strand, within ECD_00219at 250.304 kb on + strand, within ECD_00219at 250.305 kb on - strand, within ECD_00219at 250.305 kb on - strand, within ECD_00219at 250.363 kb on - strandat 250.386 kb on + strandat 250.387 kb on - strandat 250.470 kb on - strandat 250.599 kb on + strand, within ECD_00220

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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247,951 - -1.6
247,974 - +0.7
248,092 + +0.9
248,121 - +0.8
248,377 + ECD_00217 0.45 +0.8
248,776 - -0.2
248,968 + -0.3
248,968 + +0.3
248,968 + -0.4
248,996 + ECD_00218 0.12 +0.1
248,997 - ECD_00218 0.12 +0.9
249,021 + ECD_00218 0.16 -1.2
249,048 - ECD_00218 0.19 -1.1
249,068 + ECD_00218 0.22 +0.8
249,213 + ECD_00218 0.41 -0.2
249,213 + ECD_00218 0.41 +0.5
249,374 + ECD_00218 0.62 +0.5
249,442 - ECD_00218 0.70 -1.4
249,442 - ECD_00218 0.70 -1.1
249,446 - ECD_00218 0.71 -1.0
249,446 - ECD_00218 0.71 -1.4
249,446 - ECD_00218 0.71 -1.4
249,465 - ECD_00218 0.73 -2.3
249,661 + +0.1
249,662 - -1.0
249,719 - ECD_00219 0.11 -0.7
249,753 - ECD_00219 0.15 +0.3
249,817 + ECD_00219 0.24 +2.1
249,818 - ECD_00219 0.24 -0.4
249,863 + ECD_00219 0.30 +0.1
249,863 + ECD_00219 0.30 -2.8
249,989 - ECD_00219 0.47 -0.2
250,019 - ECD_00219 0.51 +0.3
250,019 - ECD_00219 0.51 -1.8
250,038 - ECD_00219 0.54 -0.6
250,041 - ECD_00219 0.54 -0.2
250,051 + ECD_00219 0.56 -2.5
250,052 - ECD_00219 0.56 -0.8
250,208 - ECD_00219 0.77 +0.3
250,208 - ECD_00219 0.77 -0.6
250,221 - ECD_00219 0.79 +0.7
250,221 - ECD_00219 0.79 -1.2
250,274 + ECD_00219 0.86 -1.2
250,274 + ECD_00219 0.86 -1.4
250,275 - ECD_00219 0.86 +0.5
250,275 - ECD_00219 0.86 +1.0
250,275 - ECD_00219 0.86 +0.3
250,275 - ECD_00219 0.86 -0.0
250,275 - ECD_00219 0.86 -0.2
250,304 + ECD_00219 0.90 -0.8
250,305 - ECD_00219 0.90 -0.4
250,305 - ECD_00219 0.90 -0.1
250,363 - -0.4
250,386 + -2.8
250,387 - -0.5
250,470 - +0.6
250,599 + ECD_00220 0.23 +0.2

Or see this region's nucleotide sequence