Strain Fitness in Escherichia coli BL21 around ECD_00126

Experiment: Mixed culture, Bdellovibrio bacteriovorus HD100_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00124 and ECD_00125 are separated by 40 nucleotidesECD_00125 and ECD_00126 are separated by 108 nucleotidesECD_00126 and ECD_00127 overlap by 4 nucleotidesECD_00127 and ECD_00128 are separated by 104 nucleotides ECD_00124: ECD_00124 - hypoxanthine phosphoribosyltransferase, at 144,235 to 144,771 _00124 ECD_00125: ECD_00125 - carbonic anhydrase, at 144,812 to 145,474 _00125 ECD_00126: ECD_00126 - putative ABC transporter ATPase, at 145,583 to 146,509 _00126 ECD_00127: ECD_00127 - putative ABC transporter permease, at 146,506 to 147,276 _00127 ECD_00128: ECD_00128 - putative PTS Enzyme IIA, at 147,381 to 147,821 _00128 Position (kb) 145 146 147Strain fitness (log2 ratio) -3 -2 -1 0 1at 144.713 kb on - strand, within ECD_00124at 144.739 kb on + strandat 144.769 kb on + strandat 144.770 kb on - strandat 145.557 kb on + strandat 145.557 kb on + strandat 145.558 kb on - strandat 145.566 kb on - strandat 145.570 kb on + strandat 145.571 kb on - strandat 145.571 kb on - strandat 145.653 kb on + strandat 145.653 kb on + strandat 145.732 kb on + strand, within ECD_00126at 145.747 kb on - strand, within ECD_00126at 145.750 kb on + strand, within ECD_00126at 145.773 kb on + strand, within ECD_00126at 145.773 kb on + strand, within ECD_00126at 145.895 kb on + strand, within ECD_00126at 145.895 kb on + strand, within ECD_00126at 145.992 kb on + strand, within ECD_00126at 145.993 kb on - strand, within ECD_00126at 146.047 kb on + strand, within ECD_00126at 146.278 kb on + strand, within ECD_00126at 146.395 kb on - strand, within ECD_00126at 146.398 kb on + strand, within ECD_00126at 146.407 kb on - strand, within ECD_00126at 146.427 kb on + strandat 146.430 kb on + strandat 146.430 kb on + strandat 146.431 kb on - strandat 146.434 kb on + strandat 146.571 kb on + strandat 146.625 kb on + strand, within ECD_00127at 146.652 kb on + strand, within ECD_00127at 146.677 kb on + strand, within ECD_00127at 146.677 kb on + strand, within ECD_00127at 146.678 kb on - strand, within ECD_00127at 146.678 kb on - strand, within ECD_00127at 146.679 kb on + strand, within ECD_00127at 146.732 kb on - strand, within ECD_00127at 146.775 kb on + strand, within ECD_00127at 146.776 kb on - strand, within ECD_00127at 146.776 kb on - strand, within ECD_00127at 146.779 kb on - strand, within ECD_00127at 147.053 kb on - strand, within ECD_00127at 147.131 kb on - strand, within ECD_00127at 147.133 kb on - strand, within ECD_00127at 147.133 kb on - strand, within ECD_00127at 147.133 kb on - strand, within ECD_00127at 147.213 kb on + strandat 147.274 kb on + strandat 147.274 kb on + strandat 147.275 kb on - strandat 147.280 kb on + strandat 147.351 kb on + strandat 147.351 kb on + strandat 147.352 kb on - strandat 147.398 kb on + strandat 147.399 kb on - strandat 147.399 kb on - strandat 147.503 kb on + strand, within ECD_00128at 147.504 kb on - strand, within ECD_00128at 147.506 kb on + strand, within ECD_00128at 147.506 kb on + strand, within ECD_00128at 147.507 kb on - strand, within ECD_00128

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Bdellovibrio bacteriovorus HD100_1
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144,713 - ECD_00124 0.89 -2.1
144,739 + -2.6
144,769 + +0.3
144,770 - -1.6
145,557 + +0.9
145,557 + +0.4
145,558 - -1.3
145,566 - -1.0
145,570 + -0.0
145,571 - -1.4
145,571 - -1.6
145,653 + -0.8
145,653 + +0.1
145,732 + ECD_00126 0.16 -0.4
145,747 - ECD_00126 0.18 -0.0
145,750 + ECD_00126 0.18 -0.4
145,773 + ECD_00126 0.20 -0.8
145,773 + ECD_00126 0.20 -0.3
145,895 + ECD_00126 0.34 -0.1
145,895 + ECD_00126 0.34 -0.4
145,992 + ECD_00126 0.44 -0.3
145,993 - ECD_00126 0.44 -3.0
146,047 + ECD_00126 0.50 -0.9
146,278 + ECD_00126 0.75 +0.1
146,395 - ECD_00126 0.88 -2.5
146,398 + ECD_00126 0.88 -0.1
146,407 - ECD_00126 0.89 +0.4
146,427 + +0.3
146,430 + -0.1
146,430 + +0.6
146,431 - -0.9
146,434 + +0.2
146,571 + +0.1
146,625 + ECD_00127 0.15 +0.7
146,652 + ECD_00127 0.19 -0.2
146,677 + ECD_00127 0.22 +0.8
146,677 + ECD_00127 0.22 -0.2
146,678 - ECD_00127 0.22 -0.6
146,678 - ECD_00127 0.22 -0.1
146,679 + ECD_00127 0.22 -1.5
146,732 - ECD_00127 0.29 -0.8
146,775 + ECD_00127 0.35 -0.7
146,776 - ECD_00127 0.35 -1.3
146,776 - ECD_00127 0.35 -1.3
146,779 - ECD_00127 0.35 +0.0
147,053 - ECD_00127 0.71 +0.6
147,131 - ECD_00127 0.81 -0.5
147,133 - ECD_00127 0.81 -0.9
147,133 - ECD_00127 0.81 -0.8
147,133 - ECD_00127 0.81 -2.9
147,213 + +0.6
147,274 + +0.7
147,274 + -0.8
147,275 - -1.6
147,280 + -0.2
147,351 + +0.3
147,351 + +0.9
147,352 - -1.3
147,398 + +0.8
147,399 - -3.2
147,399 - -1.8
147,503 + ECD_00128 0.28 -1.5
147,504 - ECD_00128 0.28 -1.6
147,506 + ECD_00128 0.28 -0.4
147,506 + ECD_00128 0.28 +0.1
147,507 - ECD_00128 0.29 -2.3

Or see this region's nucleotide sequence