Experiment: mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2002 and PP_t31 are separated by 219 nucleotides PP_t31 and PP_t32 are separated by 65 nucleotides PP_t32 and PP_t33 are separated by 99 nucleotides PP_t33 and PP_t34 are separated by 141 nucleotides PP_t34 and PP_2003 are separated by 83 nucleotides PP_2003 and PP_2004 are separated by 290 nucleotides
PP_2002: PP_2002 - putative enzyme, at 2,270,406 to 2,271,179
_2002
PP_t31: PP_t31 - tRNA-Val, at 2,271,399 to 2,271,474
_t31
PP_t32: PP_t32 - tRNA-Asp, at 2,271,540 to 2,271,616
_t32
PP_t33: PP_t33 - tRNA-Asp, at 2,271,716 to 2,271,792
_t33
PP_t34: PP_t34 - tRNA-Asp, at 2,271,934 to 2,272,010
_t34
PP_2003: PP_2003 - conserved protein of unknown function, at 2,272,094 to 2,273,035
_2003
PP_2004: PP_2004 - Transcriptional regulator, AraC family, at 2,273,326 to 2,274,366
_2004
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.173 kb on + strand at 2271.173 kb on + strand at 2271.174 kb on - strand at 2271.227 kb on + strand at 2271.228 kb on - strand at 2271.236 kb on + strand at 2271.249 kb on + strand at 2271.249 kb on + strand at 2271.250 kb on - strand at 2271.724 kb on - strand, within PP_t33 at 2271.811 kb on + strand at 2271.812 kb on - strand at 2271.812 kb on - strand at 2271.896 kb on - strand at 2271.896 kb on - strand at 2271.942 kb on - strand, within PP_t34 at 2272.179 kb on + strand at 2272.180 kb on - strand at 2272.180 kb on - strand at 2272.180 kb on - strand at 2272.328 kb on - strand, within PP_2003 at 2272.403 kb on + strand, within PP_2003 at 2272.403 kb on + strand, within PP_2003 at 2272.404 kb on - strand, within PP_2003 at 2272.694 kb on + strand, within PP_2003 at 2272.703 kb on + strand, within PP_2003 at 2272.704 kb on - strand, within PP_2003 at 2272.724 kb on + strand, within PP_2003 at 2272.724 kb on + strand, within PP_2003 at 2272.725 kb on - strand, within PP_2003 at 2272.725 kb on - strand, within PP_2003 at 2273.093 kb on + strand at 2273.094 kb on - strand at 2273.107 kb on + strand at 2273.107 kb on + strand at 2273.108 kb on - strand at 2273.108 kb on - strand at 2273.142 kb on + strand at 2273.149 kb on - strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.157 kb on + strand at 2273.158 kb on - strand at 2273.158 kb on - strand at 2273.158 kb on - strand at 2273.158 kb on - strand at 2273.190 kb on + strand at 2273.190 kb on + strand at 2273.208 kb on + strand at 2273.208 kb on + strand at 2273.208 kb on + strand at 2273.209 kb on - strand at 2273.209 kb on - strand at 2273.209 kb on - strand at 2273.209 kb on - strand at 2273.209 kb on - strand at 2273.253 kb on - strand at 2273.265 kb on + strand at 2273.265 kb on + strand at 2273.282 kb on + strand at 2273.328 kb on - strand at 2273.429 kb on + strand at 2273.429 kb on + strand at 2273.429 kb on + strand at 2273.429 kb on + strand at 2273.430 kb on - strand, within PP_2004 at 2273.430 kb on - strand, within PP_2004 at 2273.430 kb on - strand, within PP_2004 at 2273.430 kb on - strand, within PP_2004 at 2273.430 kb on - strand, within PP_2004 at 2273.434 kb on + strand, within PP_2004 at 2273.435 kb on - strand, within PP_2004 at 2273.435 kb on - strand, within PP_2004 at 2273.468 kb on + strand, within PP_2004 at 2273.468 kb on + strand, within PP_2004 at 2273.468 kb on + strand, within PP_2004 at 2273.469 kb on - strand, within PP_2004 at 2273.469 kb on - strand, within PP_2004 at 2273.469 kb on - strand, within PP_2004 at 2273.560 kb on + strand, within PP_2004 at 2273.560 kb on + strand, within PP_2004 at 2273.561 kb on - strand, within PP_2004 at 2273.816 kb on + strand, within PP_2004 at 2273.828 kb on + strand, within PP_2004 at 2273.828 kb on + strand, within PP_2004 at 2273.828 kb on + strand, within PP_2004 at 2273.829 kb on - strand at 2273.942 kb on + strand, within PP_2004 at 2273.951 kb on + strand, within PP_2004 at 2273.951 kb on + strand, within PP_2004 at 2273.951 kb on + strand, within PP_2004 at 2273.966 kb on + strand, within PP_2004 at 2273.966 kb on + strand, within PP_2004 at 2273.966 kb on + strand, within PP_2004
Per-strain Table
Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM remove 2,271,171 + +1.2 2,271,171 + -0.6 2,271,171 + -1.2 2,271,171 + -2.2 2,271,171 + -0.8 2,271,171 + +0.3 2,271,171 + -1.5 2,271,171 + -0.6 2,271,172 - -0.6 2,271,172 - -0.8 2,271,172 - +1.0 2,271,172 - +0.5 2,271,172 - +0.5 2,271,172 - -1.4 2,271,172 - +0.0 2,271,172 - -1.4 2,271,173 + +0.7 2,271,173 + +0.4 2,271,174 - -1.0 2,271,227 + -1.0 2,271,228 - -0.0 2,271,236 + -0.4 2,271,249 + +0.5 2,271,249 + -1.0 2,271,250 - -0.3 2,271,724 - PP_t33 0.10 +2.4 2,271,811 + -0.2 2,271,812 - +0.4 2,271,812 - +0.4 2,271,896 - +1.0 2,271,896 - +0.4 2,271,942 - PP_t34 0.10 +0.1 2,272,179 + +0.0 2,272,180 - +0.2 2,272,180 - +0.0 2,272,180 - -0.4 2,272,328 - PP_2003 0.25 -0.9 2,272,403 + PP_2003 0.33 -1.3 2,272,403 + PP_2003 0.33 -1.0 2,272,404 - PP_2003 0.33 -0.7 2,272,694 + PP_2003 0.64 -0.6 2,272,703 + PP_2003 0.65 -0.6 2,272,704 - PP_2003 0.65 -1.0 2,272,724 + PP_2003 0.67 -1.2 2,272,724 + PP_2003 0.67 +0.8 2,272,725 - PP_2003 0.67 -0.5 2,272,725 - PP_2003 0.67 -0.9 2,273,093 + +0.2 2,273,094 - +1.1 2,273,107 + +0.3 2,273,107 + +0.6 2,273,108 - -0.9 2,273,108 - +0.2 2,273,142 + +0.8 2,273,149 - +0.3 2,273,157 + -0.5 2,273,157 + -0.4 2,273,157 + +1.4 2,273,157 + -0.1 2,273,157 + -1.4 2,273,157 + -0.3 2,273,157 + +0.0 2,273,158 - +0.7 2,273,158 - -0.7 2,273,158 - +1.6 2,273,158 - +0.2 2,273,190 + +0.8 2,273,190 + +0.7 2,273,208 + +0.1 2,273,208 + -0.4 2,273,208 + +0.7 2,273,209 - +0.0 2,273,209 - -0.8 2,273,209 - -2.0 2,273,209 - +0.0 2,273,209 - +0.1 2,273,253 - +1.0 2,273,265 + +0.1 2,273,265 + -0.1 2,273,282 + +0.4 2,273,328 - +0.7 2,273,429 + -0.7 2,273,429 + +0.0 2,273,429 + -0.7 2,273,429 + -0.6 2,273,430 - PP_2004 0.10 +0.3 2,273,430 - PP_2004 0.10 +0.5 2,273,430 - PP_2004 0.10 -0.5 2,273,430 - PP_2004 0.10 -0.9 2,273,430 - PP_2004 0.10 +0.1 2,273,434 + PP_2004 0.10 +0.1 2,273,435 - PP_2004 0.10 +0.8 2,273,435 - PP_2004 0.10 -1.6 2,273,468 + PP_2004 0.14 +0.2 2,273,468 + PP_2004 0.14 +0.9 2,273,468 + PP_2004 0.14 +0.5 2,273,469 - PP_2004 0.14 -1.0 2,273,469 - PP_2004 0.14 +0.5 2,273,469 - PP_2004 0.14 -0.2 2,273,560 + PP_2004 0.22 +0.5 2,273,560 + PP_2004 0.22 -0.8 2,273,561 - PP_2004 0.23 -0.6 2,273,816 + PP_2004 0.47 -0.1 2,273,828 + PP_2004 0.48 -1.6 2,273,828 + PP_2004 0.48 +0.3 2,273,828 + PP_2004 0.48 -0.7 2,273,829 - +3.0 2,273,942 + PP_2004 0.59 +0.5 2,273,951 + PP_2004 0.60 -0.2 2,273,951 + PP_2004 0.60 -0.4 2,273,951 + PP_2004 0.60 +0.8 2,273,966 + PP_2004 0.61 -0.5 2,273,966 + PP_2004 0.61 +0.4 2,273,966 + PP_2004 0.61 +0.6
Or see this region's nucleotide sequence