Strain Fitness in Pseudomonas putida KT2440 around PP_0825

Experiment: mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0823 and PP_0824 are separated by 126 nucleotidesPP_0824 and PP_0825 overlap by 4 nucleotidesPP_0825 and PP_0826 overlap by 7 nucleotidesPP_0826 and PP_0827 overlap by 4 nucleotides PP_0823: PP_0823 - Selenide, water dikinase, at 961,380 to 962,414 _0823 PP_0824: PP_0824 - phosphonate transport system-binding protein, at 962,541 to 963,395 _0824 PP_0825: PP_0825 - phosphonates import ATP-binding protein, at 963,392 to 964,201 _0825 PP_0826: PP_0826 - phosphonate ABC transporter, permease protein, at 964,195 to 965,022 _0826 PP_0827: PP_0827 - putative phosphonate transport system permease protein PtxC, at 965,019 to 965,786 _0827 Position (kb) 963 964 965Strain fitness (log2 ratio) -2 -1 0 1 2at 962.414 kb on + strandat 962.414 kb on + strandat 962.450 kb on - strandat 962.450 kb on - strandat 962.466 kb on - strandat 962.480 kb on + strandat 962.481 kb on - strandat 962.481 kb on - strandat 962.483 kb on - strandat 962.483 kb on - strandat 962.725 kb on + strand, within PP_0824at 962.725 kb on + strand, within PP_0824at 962.849 kb on - strand, within PP_0824at 962.849 kb on - strand, within PP_0824at 962.986 kb on - strand, within PP_0824at 963.008 kb on + strand, within PP_0824at 963.009 kb on - strand, within PP_0824at 963.024 kb on + strand, within PP_0824at 963.025 kb on - strand, within PP_0824at 963.189 kb on + strand, within PP_0824at 963.190 kb on - strand, within PP_0824at 963.199 kb on - strand, within PP_0824at 963.290 kb on + strand, within PP_0824at 963.291 kb on - strand, within PP_0824at 963.348 kb on + strandat 963.348 kb on + strandat 963.348 kb on + strandat 963.348 kb on + strandat 963.349 kb on - strandat 963.349 kb on - strandat 963.349 kb on - strandat 963.349 kb on - strandat 963.422 kb on + strandat 963.450 kb on + strandat 963.479 kb on - strand, within PP_0825at 963.887 kb on + strand, within PP_0825at 963.887 kb on + strand, within PP_0825at 963.955 kb on + strand, within PP_0825at 963.956 kb on - strand, within PP_0825at 963.956 kb on - strand, within PP_0825at 964.023 kb on + strand, within PP_0825at 964.265 kb on + strandat 964.266 kb on - strandat 964.399 kb on + strand, within PP_0826at 964.400 kb on - strand, within PP_0826at 964.407 kb on + strand, within PP_0826at 964.587 kb on + strand, within PP_0826at 964.588 kb on - strand, within PP_0826at 964.588 kb on - strand, within PP_0826at 964.655 kb on + strand, within PP_0826at 964.656 kb on - strand, within PP_0826at 964.675 kb on + strandat 964.675 kb on + strand, within PP_0826at 964.675 kb on + strand, within PP_0826at 964.675 kb on + strand, within PP_0826at 964.676 kb on - strand, within PP_0826at 964.676 kb on - strand, within PP_0826at 964.700 kb on - strand, within PP_0826at 964.784 kb on - strand, within PP_0826at 964.822 kb on + strand, within PP_0826at 964.822 kb on + strand, within PP_0826at 964.823 kb on - strand, within PP_0826at 964.831 kb on + strand, within PP_0826at 965.085 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
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962,414 + +0.2
962,414 + -0.3
962,450 - -0.3
962,450 - +0.1
962,466 - +0.0
962,480 + +1.2
962,481 - -0.7
962,481 - +1.8
962,483 - +0.2
962,483 - +0.2
962,725 + PP_0824 0.22 -0.4
962,725 + PP_0824 0.22 +0.5
962,849 - PP_0824 0.36 +0.2
962,849 - PP_0824 0.36 -0.3
962,986 - PP_0824 0.52 +0.9
963,008 + PP_0824 0.55 +0.2
963,009 - PP_0824 0.55 -0.1
963,024 + PP_0824 0.56 +0.7
963,025 - PP_0824 0.57 +0.3
963,189 + PP_0824 0.76 +0.1
963,190 - PP_0824 0.76 -0.1
963,199 - PP_0824 0.77 +0.3
963,290 + PP_0824 0.88 -1.3
963,291 - PP_0824 0.88 -1.2
963,348 + +0.9
963,348 + -0.1
963,348 + -2.6
963,348 + +0.9
963,349 - -0.1
963,349 - -0.7
963,349 - +0.5
963,349 - -0.8
963,422 + -0.8
963,450 + +0.4
963,479 - PP_0825 0.11 +1.3
963,887 + PP_0825 0.61 -0.9
963,887 + PP_0825 0.61 +0.4
963,955 + PP_0825 0.70 -1.8
963,956 - PP_0825 0.70 -1.5
963,956 - PP_0825 0.70 -0.0
964,023 + PP_0825 0.78 -0.0
964,265 + -0.0
964,266 - +0.3
964,399 + PP_0826 0.25 +0.1
964,400 - PP_0826 0.25 -1.1
964,407 + PP_0826 0.26 -0.1
964,587 + PP_0826 0.47 -0.4
964,588 - PP_0826 0.47 +0.1
964,588 - PP_0826 0.47 +0.0
964,655 + PP_0826 0.56 -0.6
964,656 - PP_0826 0.56 -0.7
964,675 + -0.0
964,675 + PP_0826 0.58 +0.0
964,675 + PP_0826 0.58 -0.6
964,675 + PP_0826 0.58 -0.6
964,676 - PP_0826 0.58 -2.0
964,676 - PP_0826 0.58 -1.1
964,700 - PP_0826 0.61 +0.1
964,784 - PP_0826 0.71 +0.4
964,822 + PP_0826 0.76 -0.9
964,822 + PP_0826 0.76 +0.3
964,823 - PP_0826 0.76 -0.9
964,831 + PP_0826 0.77 +0.4
965,085 + -1.4

Or see this region's nucleotide sequence