Experiment: mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4636 and PP_4637 are separated by 138 nucleotides PP_4637 and PP_4638 are separated by 227 nucleotides
PP_4636: PP_4636 - acetyl-CoA acetyltransferase, at 5,259,459 to 5,260,637
_4636
PP_4637: PP_4637 - 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase family protein, at 5,260,776 to 5,261,600
_4637
PP_4638: PP_4638 - Methylenetetrahydrofolate reductase, at 5,261,828 to 5,263,315
_4638
Position (kb) 5260
5261
5262 Strain fitness (log2 ratio) -2
-1
0
1 at 5259.826 kb on - strand, within PP_4636 at 5259.826 kb on - strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.922 kb on - strand, within PP_4636 at 5259.922 kb on - strand, within PP_4636 at 5259.961 kb on - strand, within PP_4636 at 5259.962 kb on - strand, within PP_4636 at 5260.682 kb on - strand at 5260.731 kb on - strand at 5260.992 kb on + strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5261.030 kb on - strand, within PP_4637 at 5261.119 kb on + strand, within PP_4637 at 5261.165 kb on - strand, within PP_4637 at 5261.176 kb on + strand, within PP_4637 at 5261.176 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.350 kb on + strand, within PP_4637 at 5261.350 kb on + strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.462 kb on + strand, within PP_4637 at 5261.462 kb on + strand, within PP_4637 at 5261.463 kb on - strand, within PP_4637 at 5261.585 kb on + strand at 5261.585 kb on + strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.827 kb on + strand at 5261.828 kb on - strand at 5261.875 kb on - strand at 5261.875 kb on - strand at 5262.138 kb on + strand, within PP_4638 at 5262.139 kb on - strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.394 kb on - strand, within PP_4638 at 5262.394 kb on - strand, within PP_4638 at 5262.592 kb on + strand, within PP_4638 at 5262.598 kb on + strand, within PP_4638 at 5262.599 kb on - strand, within PP_4638
Per-strain Table
Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM remove 5,259,826 - PP_4636 0.31 +0.6 5,259,826 - PP_4636 0.31 -0.8 5,259,921 + PP_4636 0.39 -0.4 5,259,921 + PP_4636 0.39 +0.1 5,259,921 + PP_4636 0.39 -1.6 5,259,922 - PP_4636 0.39 -0.0 5,259,922 - PP_4636 0.39 -0.0 5,259,961 - PP_4636 0.43 +0.4 5,259,962 - PP_4636 0.43 -0.4 5,260,682 - +0.1 5,260,731 - -0.5 5,260,992 + PP_4637 0.26 +1.0 5,260,993 - PP_4637 0.26 -0.5 5,260,993 - PP_4637 0.26 -0.2 5,260,993 - PP_4637 0.26 -1.4 5,261,030 - PP_4637 0.31 -0.2 5,261,119 + PP_4637 0.42 -1.1 5,261,165 - PP_4637 0.47 -1.5 5,261,176 + PP_4637 0.48 +0.2 5,261,176 + PP_4637 0.48 -0.9 5,261,278 + PP_4637 0.61 -1.4 5,261,278 + PP_4637 0.61 +1.4 5,261,278 + PP_4637 0.61 -0.2 5,261,279 - PP_4637 0.61 +0.3 5,261,279 - PP_4637 0.61 +0.1 5,261,279 - PP_4637 0.61 +1.7 5,261,279 - PP_4637 0.61 -0.2 5,261,279 - PP_4637 0.61 -1.2 5,261,350 + PP_4637 0.70 -1.0 5,261,350 + PP_4637 0.70 +0.6 5,261,351 - PP_4637 0.70 +0.1 5,261,351 - PP_4637 0.70 -0.9 5,261,351 - PP_4637 0.70 +0.2 5,261,462 + PP_4637 0.83 -0.3 5,261,462 + PP_4637 0.83 -0.2 5,261,463 - PP_4637 0.83 +0.1 5,261,585 + -0.9 5,261,585 + +0.8 5,261,586 - -0.6 5,261,586 - +0.1 5,261,586 - -0.5 5,261,586 - -1.2 5,261,827 + +0.5 5,261,828 - -0.3 5,261,875 - -1.5 5,261,875 - -0.4 5,262,138 + PP_4638 0.21 -0.2 5,262,139 - PP_4638 0.21 +0.4 5,262,305 + PP_4638 0.32 -1.9 5,262,305 + PP_4638 0.32 -0.2 5,262,305 + PP_4638 0.32 +0.5 5,262,394 - PP_4638 0.38 -0.4 5,262,394 - PP_4638 0.38 -0.0 5,262,592 + PP_4638 0.51 -1.6 5,262,598 + PP_4638 0.52 -0.3 5,262,599 - PP_4638 0.52 -0.5
Or see this region's nucleotide sequence