Experiment: mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0527 and PP_0528 are separated by 177 nucleotides PP_0528 and PP_0529 overlap by 4 nucleotides PP_0529 and PP_0530 are separated by 165 nucleotides
PP_0527: PP_0527 - 1-deoxyxylulose-5-phosphate synthase, at 612,286 to 614,181
_0527
PP_0528: PP_0528 - farnesyl diphosphate synthase, at 614,359 to 615,246
_0528
PP_0529: PP_0529 - Exodeoxyribonuclease 7 small subunit, at 615,243 to 615,485
_0529
PP_0530: PP_0530 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 615,651 to 616,301
_0530
Position (kb) 614
615
616 Strain fitness (log2 ratio) -1
0
1
2 at 614.195 kb on - strand at 614.195 kb on - strand at 614.197 kb on - strand at 614.197 kb on - strand at 614.197 kb on - strand at 614.197 kb on - strand at 614.273 kb on - strand at 614.273 kb on - strand at 614.273 kb on - strand at 614.339 kb on - strand at 614.339 kb on - strand at 614.346 kb on + strand at 614.346 kb on + strand at 614.347 kb on - strand at 614.347 kb on - strand at 615.730 kb on + strand, within PP_0530 at 615.742 kb on + strand, within PP_0530 at 615.742 kb on + strand, within PP_0530 at 615.742 kb on + strand, within PP_0530 at 615.742 kb on + strand, within PP_0530 at 615.743 kb on - strand, within PP_0530 at 615.744 kb on + strand, within PP_0530 at 615.744 kb on + strand, within PP_0530 at 615.744 kb on + strand, within PP_0530 at 615.854 kb on + strand, within PP_0530 at 615.924 kb on - strand, within PP_0530 at 615.924 kb on - strand, within PP_0530 at 616.001 kb on + strand, within PP_0530 at 616.002 kb on - strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.163 kb on + strand, within PP_0530 at 616.164 kb on - strand, within PP_0530 at 616.164 kb on - strand, within PP_0530 at 616.227 kb on + strand, within PP_0530 at 616.228 kb on - strand, within PP_0530 at 616.228 kb on - strand, within PP_0530
Per-strain Table
Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM remove 614,195 - +0.9 614,195 - +1.5 614,197 - +0.3 614,197 - +0.3 614,197 - +0.7 614,197 - +2.3 614,273 - -0.2 614,273 - +0.1 614,273 - +0.9 614,339 - -0.4 614,339 - +1.0 614,346 + +0.7 614,346 + -0.3 614,347 - +0.3 614,347 - -0.6 615,730 + PP_0530 0.12 +0.7 615,742 + PP_0530 0.14 -0.1 615,742 + PP_0530 0.14 +0.3 615,742 + PP_0530 0.14 +0.6 615,742 + PP_0530 0.14 +0.0 615,743 - PP_0530 0.14 -0.7 615,744 + PP_0530 0.14 +0.7 615,744 + PP_0530 0.14 +0.7 615,744 + PP_0530 0.14 -1.1 615,854 + PP_0530 0.31 +0.1 615,924 - PP_0530 0.42 -0.5 615,924 - PP_0530 0.42 -1.0 616,001 + PP_0530 0.54 +1.6 616,002 - PP_0530 0.54 -0.4 616,163 + PP_0530 0.79 +0.2 616,163 + PP_0530 0.79 -0.9 616,163 + PP_0530 0.79 -0.7 616,163 + PP_0530 0.79 -0.2 616,163 + PP_0530 0.79 -0.2 616,163 + PP_0530 0.79 -0.9 616,163 + PP_0530 0.79 +1.5 616,163 + PP_0530 0.79 -0.9 616,164 - PP_0530 0.79 -0.5 616,164 - PP_0530 0.79 +0.2 616,227 + PP_0530 0.88 -0.5 616,228 - PP_0530 0.89 +0.3 616,228 - PP_0530 0.89 -1.1
Or see this region's nucleotide sequence