Experiment: mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0184 and PP_0185 are separated by 285 nucleotides PP_0185 and PP_0186 are separated by 118 nucleotides
PP_0184: PP_0184 - Argininosuccinate lyase, at 235,428 to 236,834
_0184
PP_0185: PP_0185 - Positive alginate biosynthesis regulatory protein, at 237,120 to 237,863
_0185
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
Position (kb) 237
238 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 236.269 kb on + strand, within PP_0184 at 236.269 kb on + strand, within PP_0184 at 236.269 kb on + strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.270 kb on - strand, within PP_0184 at 236.495 kb on + strand, within PP_0184 at 236.496 kb on - strand, within PP_0184 at 236.496 kb on - strand, within PP_0184 at 236.717 kb on - strand at 236.867 kb on + strand at 236.867 kb on + strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.868 kb on - strand at 236.971 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.076 kb on + strand at 237.077 kb on - strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.081 kb on + strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.082 kb on - strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.083 kb on + strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.084 kb on - strand at 237.119 kb on + strand at 237.119 kb on + strand at 237.120 kb on - strand at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.195 kb on + strand, within PP_0185 at 237.196 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.379 kb on - strand, within PP_0185 at 237.540 kb on - strand, within PP_0185 at 237.594 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.615 kb on + strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.616 kb on - strand, within PP_0185 at 237.781 kb on - strand, within PP_0185 at 237.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM remove 236,269 + PP_0184 0.60 +0.9 236,269 + PP_0184 0.60 +1.5 236,269 + PP_0184 0.60 +0.1 236,270 - PP_0184 0.60 +1.5 236,270 - PP_0184 0.60 +0.9 236,270 - PP_0184 0.60 -0.5 236,495 + PP_0184 0.76 +0.5 236,496 - PP_0184 0.76 +0.1 236,496 - PP_0184 0.76 +0.9 236,717 - +2.5 236,867 + +0.5 236,867 + -0.7 236,868 - +0.1 236,868 - -1.3 236,868 - -0.5 236,868 - +0.3 236,868 - -0.7 236,971 + +1.5 237,076 + +1.0 237,076 + +0.7 237,076 + -1.4 237,077 - +0.0 237,081 + -0.1 237,081 + -0.3 237,081 + -0.1 237,082 - -2.2 237,082 - -3.3 237,082 - +0.9 237,083 + -1.8 237,083 + +1.5 237,083 + -0.1 237,083 + +0.4 237,084 - -1.6 237,084 - -2.5 237,084 - -1.0 237,084 - -2.6 237,084 - -0.5 237,119 + +0.1 237,119 + -0.7 237,120 - -1.5 237,195 + PP_0185 0.10 -1.9 237,195 + PP_0185 0.10 -0.7 237,195 + PP_0185 0.10 -2.4 237,196 - PP_0185 0.10 -1.6 237,379 - PP_0185 0.35 -2.1 237,379 - PP_0185 0.35 -1.0 237,379 - PP_0185 0.35 -3.2 237,540 - PP_0185 0.56 -2.2 237,594 + PP_0185 0.64 -1.6 237,615 + PP_0185 0.67 -1.9 237,615 + PP_0185 0.67 -0.9 237,615 + PP_0185 0.67 -2.5 237,615 + PP_0185 0.67 -3.2 237,615 + PP_0185 0.67 -0.3 237,615 + +1.5 237,615 + PP_0185 0.67 -1.9 237,615 + PP_0185 0.67 -2.1 237,615 + PP_0185 0.67 -0.5 237,616 - PP_0185 0.67 -2.2 237,616 - PP_0185 0.67 -1.9 237,616 - PP_0185 0.67 -0.5 237,616 - PP_0185 0.67 -1.9 237,616 - PP_0185 0.67 -2.4 237,781 - PP_0185 0.89 -1.3 237,902 - +0.0
Or see this region's nucleotide sequence