Strain Fitness in Escherichia coli BW25113 around b2822

Experiment: Koxy_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntptr and recC are separated by 175 nucleotidesrecC and ppdC are separated by 12 nucleotidesppdC and ygdB overlap by 16 nucleotidesygdB and ppdB overlap by 4 nucleotides b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906 ptr b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450 recC b2823: ppdC - hypothetical protein (NCBI), at 2,960,463 to 2,960,786 ppdC b2824: ygdB - orf, hypothetical protein (VIMSS), at 2,960,771 to 2,961,178 ygdB b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738 ppdB Position (kb) 2957 2958 2959 2960 2961Strain fitness (log2 ratio) -2 -1 0 1 2at 2956.082 kb on - strand, within ptrat 2956.082 kb on - strand, within ptrat 2956.353 kb on - strand, within ptrat 2956.360 kb on + strand, within ptrat 2956.362 kb on + strand, within ptrat 2956.370 kb on - strand, within ptrat 2956.419 kb on - strand, within ptrat 2956.478 kb on - strand, within ptrat 2956.478 kb on - strand, within ptrat 2956.485 kb on + strand, within ptrat 2956.485 kb on + strand, within ptrat 2956.598 kb on - strand, within ptrat 2956.607 kb on - strand, within ptrat 2956.653 kb on + strandat 2956.658 kb on - strandat 2956.658 kb on - strandat 2956.663 kb on + strandat 2956.772 kb on - strandat 2956.772 kb on - strandat 2956.844 kb on + strandat 2956.882 kb on - strandat 2957.218 kb on - strandat 2957.288 kb on + strandat 2957.823 kb on + strand, within recCat 2958.415 kb on - strand, within recCat 2958.570 kb on - strand, within recCat 2958.570 kb on - strand, within recCat 2958.885 kb on - strand, within recCat 2959.105 kb on + strand, within recCat 2959.345 kb on + strand, within recCat 2959.585 kb on + strand, within recCat 2959.640 kb on + strand, within recCat 2959.672 kb on + strand, within recCat 2960.337 kb on - strandat 2960.370 kb on + strandat 2960.441 kb on + strandat 2960.489 kb on + strandat 2960.523 kb on - strand, within ppdCat 2960.523 kb on - strand, within ppdCat 2960.535 kb on + strand, within ppdCat 2960.661 kb on + strand, within ppdCat 2960.726 kb on - strand, within ppdCat 2960.726 kb on - strand, within ppdCat 2960.736 kb on + strand, within ppdCat 2960.759 kb on - strandat 2960.759 kb on - strandat 2960.974 kb on - strand, within ygdBat 2961.100 kb on + strand, within ygdBat 2961.100 kb on + strand, within ygdBat 2961.102 kb on - strand, within ygdBat 2961.141 kb on + strandat 2961.173 kb on + strandat 2961.343 kb on - strand, within ppdB

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Per-strain Table

Position Strand Gene LocusTag Fraction Koxy_10
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2,956,082 - ptr b2821 0.71 +0.8
2,956,082 - ptr b2821 0.71 -0.2
2,956,353 - ptr b2821 0.81 +0.1
2,956,360 + ptr b2821 0.81 +0.7
2,956,362 + ptr b2821 0.81 +1.2
2,956,370 - ptr b2821 0.81 -0.3
2,956,419 - ptr b2821 0.83 +1.6
2,956,478 - ptr b2821 0.85 +0.1
2,956,478 - ptr b2821 0.85 +0.4
2,956,485 + ptr b2821 0.85 -0.4
2,956,485 + ptr b2821 0.85 -0.1
2,956,598 - ptr b2821 0.89 -0.5
2,956,607 - ptr b2821 0.90 -0.2
2,956,653 + -0.3
2,956,658 - -0.4
2,956,658 - -0.8
2,956,663 + +0.3
2,956,772 - -0.3
2,956,772 - -0.3
2,956,844 + +0.6
2,956,882 - -0.2
2,957,218 - +0.4
2,957,288 + +0.7
2,957,823 + recC b2822 0.22 +2.3
2,958,415 - recC b2822 0.40 -0.6
2,958,570 - recC b2822 0.44 -2.4
2,958,570 - recC b2822 0.44 +0.4
2,958,885 - recC b2822 0.54 +0.1
2,959,105 + recC b2822 0.60 -0.3
2,959,345 + recC b2822 0.67 -0.3
2,959,585 + recC b2822 0.74 -0.4
2,959,640 + recC b2822 0.76 -1.5
2,959,672 + recC b2822 0.77 -1.5
2,960,337 - +0.1
2,960,370 + +0.7
2,960,441 + +0.3
2,960,489 + +0.1
2,960,523 - ppdC b2823 0.19 +0.6
2,960,523 - ppdC b2823 0.19 -0.6
2,960,535 + ppdC b2823 0.22 +0.3
2,960,661 + ppdC b2823 0.61 +0.2
2,960,726 - ppdC b2823 0.81 -1.0
2,960,726 - ppdC b2823 0.81 -0.3
2,960,736 + ppdC b2823 0.84 +0.0
2,960,759 - -0.9
2,960,759 - +0.3
2,960,974 - ygdB b2824 0.50 +0.3
2,961,100 + ygdB b2824 0.81 -0.1
2,961,100 + ygdB b2824 0.81 +0.4
2,961,102 - ygdB b2824 0.81 +1.0
2,961,141 + +0.3
2,961,173 + -0.5
2,961,343 - ppdB b2825 0.30 -0.1

Or see this region's nucleotide sequence