Experiment: Koxy_10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 16 nucleotides fruK and fruB overlap by 1 nucleotides
b2167: fruA - fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI), at 2,257,741 to 2,259,432
fruA
b2168: fruK - 1-phosphofructokinase (NCBI), at 2,259,449 to 2,260,387
fruK
b2169: fruB - fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI), at 2,260,387 to 2,261,517
fruB
Position (kb)
2259
2260
2261 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2258.450 kb on + strand, within fruA at 2258.529 kb on - strand, within fruA at 2258.529 kb on - strand, within fruA at 2258.661 kb on + strand, within fruA at 2258.756 kb on - strand, within fruA at 2258.760 kb on - strand, within fruA at 2258.845 kb on - strand, within fruA at 2258.893 kb on - strand, within fruA at 2258.970 kb on + strand, within fruA at 2259.012 kb on + strand, within fruA at 2259.089 kb on - strand, within fruA at 2259.089 kb on - strand, within fruA at 2259.094 kb on - strand, within fruA at 2259.106 kb on + strand, within fruA at 2259.184 kb on + strand, within fruA at 2259.184 kb on + strand, within fruA at 2259.197 kb on + strand, within fruA at 2259.215 kb on - strand, within fruA at 2259.380 kb on + strand at 2259.507 kb on + strand at 2259.951 kb on - strand, within fruK at 2260.134 kb on + strand, within fruK at 2260.153 kb on + strand, within fruK at 2260.398 kb on + strand at 2260.398 kb on + strand at 2260.412 kb on - strand at 2260.416 kb on - strand at 2260.592 kb on + strand, within fruB at 2260.635 kb on - strand, within fruB at 2260.821 kb on - strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.938 kb on + strand, within fruB at 2261.006 kb on - strand, within fruB at 2261.202 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.255 kb on + strand, within fruB at 2261.330 kb on - strand, within fruB
Per-strain Table
Position Strand Gene LocusTag Fraction Koxy_10 remove 2,258,450 + fruA b2167 0.42 -0.4 2,258,529 - fruA b2167 0.47 -2.8 2,258,529 - fruA b2167 0.47 -1.2 2,258,661 + fruA b2167 0.54 -1.2 2,258,756 - fruA b2167 0.60 -0.7 2,258,760 - fruA b2167 0.60 -0.4 2,258,845 - fruA b2167 0.65 -0.2 2,258,893 - fruA b2167 0.68 -0.3 2,258,970 + fruA b2167 0.73 +0.3 2,259,012 + fruA b2167 0.75 +0.2 2,259,089 - fruA b2167 0.80 -0.8 2,259,089 - fruA b2167 0.80 -0.5 2,259,094 - fruA b2167 0.80 +0.4 2,259,106 + fruA b2167 0.81 -0.2 2,259,184 + fruA b2167 0.85 +0.4 2,259,184 + fruA b2167 0.85 +0.9 2,259,197 + fruA b2167 0.86 +0.1 2,259,215 - fruA b2167 0.87 +0.2 2,259,380 + -1.5 2,259,507 + +0.8 2,259,951 - fruK b2168 0.53 -1.3 2,260,134 + fruK b2168 0.73 +0.9 2,260,153 + fruK b2168 0.75 -1.5 2,260,398 + -0.1 2,260,398 + +0.0 2,260,412 - +0.1 2,260,416 - -0.2 2,260,592 + fruB b2169 0.18 -0.3 2,260,635 - fruB b2169 0.22 +0.1 2,260,821 - fruB b2169 0.38 -0.2 2,260,911 + fruB b2169 0.46 +0.1 2,260,911 + fruB b2169 0.46 +0.2 2,260,938 + fruB b2169 0.49 -0.4 2,261,006 - fruB b2169 0.55 +0.5 2,261,202 + fruB b2169 0.72 -0.4 2,261,241 + fruB b2169 0.76 -2.5 2,261,241 + fruB b2169 0.76 -0.5 2,261,255 + fruB b2169 0.77 -0.3 2,261,330 - fruB b2169 0.83 +0.2
Or see this region's nucleotide sequence