Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recN and smpA are separated by 148 nucleotides smpA and b2618 are separated by 61 nucleotides b2618 and yfjG overlap by 11 nucleotides yfjG and smpB are separated by 131 nucleotides
b2616: recN - recombination and repair protein (NCBI), at 2,749,817 to 2,751,478
recN
b2617: smpA - small membrane protein A (VIMSS), at 2,751,627 to 2,751,968
smpA
b2618: b2618 - orf, hypothetical protein (VIMSS), at 2,752,030 to 2,752,320
b2618
b2619: yfjG - hypothetical protein (NCBI), at 2,752,310 to 2,752,786
yfjG
b2620: smpB - SsrA-binding protein (NCBI), at 2,752,918 to 2,753,400
smpB
Position (kb)
2752
2753 Strain fitness (log2 ratio)
-1
0
1 at 2751.106 kb on - strand, within recN at 2751.114 kb on + strand, within recN at 2751.127 kb on + strand, within recN at 2751.139 kb on + strand, within recN at 2751.139 kb on + strand, within recN at 2751.274 kb on - strand, within recN at 2751.274 kb on - strand, within recN at 2751.357 kb on - strand at 2751.357 kb on - strand at 2751.363 kb on + strand at 2751.363 kb on + strand at 2751.436 kb on + strand at 2751.588 kb on + strand at 2751.588 kb on + strand at 2751.813 kb on - strand, within smpA at 2751.840 kb on + strand, within smpA at 2751.878 kb on + strand, within smpA at 2751.888 kb on - strand, within smpA at 2751.888 kb on - strand, within smpA at 2751.941 kb on + strand at 2752.081 kb on + strand, within b2618 at 2752.081 kb on + strand, within b2618 at 2752.092 kb on + strand, within b2618 at 2752.118 kb on - strand, within b2618 at 2752.150 kb on + strand, within b2618 at 2752.822 kb on + strand at 2752.851 kb on - strand at 2752.936 kb on + strand at 2753.096 kb on + strand, within smpB at 2753.223 kb on - strand, within smpB at 2753.223 kb on - strand, within smpB
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 2,751,106 - recN b2616 0.78 +0.7 2,751,114 + recN b2616 0.78 -0.8 2,751,127 + recN b2616 0.79 -0.0 2,751,139 + recN b2616 0.80 -0.4 2,751,139 + recN b2616 0.80 -1.4 2,751,274 - recN b2616 0.88 -0.5 2,751,274 - recN b2616 0.88 -0.3 2,751,357 - +0.2 2,751,357 - +0.4 2,751,363 + -0.4 2,751,363 + +0.4 2,751,436 + -0.4 2,751,588 + -0.3 2,751,588 + -0.2 2,751,813 - smpA b2617 0.54 -0.5 2,751,840 + smpA b2617 0.62 +0.5 2,751,878 + smpA b2617 0.73 +0.4 2,751,888 - smpA b2617 0.76 +0.0 2,751,888 - smpA b2617 0.76 +0.4 2,751,941 + -0.8 2,752,081 + b2618 b2618 0.18 -0.5 2,752,081 + b2618 b2618 0.18 -0.1 2,752,092 + b2618 b2618 0.21 -0.0 2,752,118 - b2618 b2618 0.30 -0.5 2,752,150 + b2618 b2618 0.41 -0.3 2,752,822 + +0.3 2,752,851 - -0.1 2,752,936 + -1.0 2,753,096 + smpB b2620 0.37 +0.4 2,753,223 - smpB b2620 0.63 -0.2 2,753,223 - smpB b2620 0.63 -1.0
Or see this region's nucleotide sequence