Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manZ and yobD are separated by 54 nucleotides yobD and b1821 are separated by 428 nucleotides b1821 and rrmA overlap by 4 nucleotides rrmA and cspC are separated by 165 nucleotides
b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770
manZ
b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283
yobD
b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278
b1821
b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084
rrmA
b1823: cspC - stress protein, member of the CspA-family (NCBI), at 1,905,250 to 1,905,459
cspC
Position (kb)
1903
1904
1905 Strain fitness (log2 ratio)
-1
0
1 at 1902.758 kb on + strand at 1902.763 kb on + strand at 1902.765 kb on + strand at 1902.765 kb on - strand at 1902.766 kb on - strand at 1902.773 kb on - strand at 1902.781 kb on - strand at 1902.867 kb on + strand at 1902.867 kb on + strand at 1902.875 kb on - strand, within yobD at 1902.875 kb on - strand at 1902.875 kb on - strand, within yobD at 1902.882 kb on + strand, within yobD at 1902.893 kb on + strand, within yobD at 1902.914 kb on + strand, within yobD at 1902.964 kb on + strand, within yobD at 1902.996 kb on + strand, within yobD at 1903.006 kb on - strand, within yobD at 1903.026 kb on - strand, within yobD at 1903.040 kb on + strand, within yobD at 1903.051 kb on + strand, within yobD at 1903.051 kb on + strand, within yobD at 1903.059 kb on - strand, within yobD at 1903.069 kb on - strand, within yobD at 1903.138 kb on + strand, within yobD at 1903.161 kb on + strand, within yobD at 1903.161 kb on + strand, within yobD at 1903.179 kb on - strand, within yobD at 1903.183 kb on + strand, within yobD at 1903.196 kb on + strand, within yobD at 1903.198 kb on + strand, within yobD at 1903.496 kb on - strand at 1903.498 kb on - strand at 1903.529 kb on + strand at 1903.537 kb on - strand at 1903.548 kb on + strand at 1903.553 kb on + strand at 1903.558 kb on + strand at 1903.558 kb on + strand at 1903.583 kb on + strand at 1903.602 kb on - strand at 1903.602 kb on - strand at 1903.639 kb on - strand at 1903.654 kb on - strand at 1903.702 kb on + strand at 1903.777 kb on - strand, within b1821 at 1903.777 kb on - strand, within b1821 at 1904.065 kb on - strand, within b1821 at 1904.087 kb on - strand, within b1821 at 1904.603 kb on + strand, within rrmA at 1904.603 kb on + strand, within rrmA at 1904.691 kb on + strand, within rrmA at 1904.897 kb on - strand, within rrmA at 1904.988 kb on - strand, within rrmA at 1905.238 kb on - strand at 1905.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 1,902,758 + +0.2 1,902,763 + +0.5 1,902,765 + +0.1 1,902,765 - +0.7 1,902,766 - +0.2 1,902,773 - -0.0 1,902,781 - -0.6 1,902,867 + -0.1 1,902,867 + +1.0 1,902,875 - yobD b1820 0.11 -0.4 1,902,875 - -0.7 1,902,875 - yobD b1820 0.11 +0.6 1,902,882 + yobD b1820 0.12 +0.3 1,902,893 + yobD b1820 0.15 +0.1 1,902,914 + yobD b1820 0.19 -0.1 1,902,964 + yobD b1820 0.30 -0.1 1,902,996 + yobD b1820 0.37 +0.5 1,903,006 - yobD b1820 0.39 +0.4 1,903,026 - yobD b1820 0.44 -0.2 1,903,040 + yobD b1820 0.47 +0.1 1,903,051 + yobD b1820 0.49 +0.3 1,903,051 + yobD b1820 0.49 -0.4 1,903,059 - yobD b1820 0.51 +0.7 1,903,069 - yobD b1820 0.53 -1.1 1,903,138 + yobD b1820 0.68 +0.4 1,903,161 + yobD b1820 0.73 +0.4 1,903,161 + yobD b1820 0.73 -0.3 1,903,179 - yobD b1820 0.77 +0.5 1,903,183 + yobD b1820 0.78 -0.3 1,903,196 + yobD b1820 0.81 -0.1 1,903,198 + yobD b1820 0.81 +0.0 1,903,496 - -0.1 1,903,498 - -0.1 1,903,529 + +0.1 1,903,537 - +0.2 1,903,548 + +0.0 1,903,553 + -0.4 1,903,558 + +0.2 1,903,558 + +0.5 1,903,583 + +0.1 1,903,602 - -0.2 1,903,602 - -0.5 1,903,639 - +0.4 1,903,654 - +0.3 1,903,702 + +0.4 1,903,777 - b1821 b1821 0.11 +0.5 1,903,777 - b1821 b1821 0.11 -0.1 1,904,065 - b1821 b1821 0.62 -0.5 1,904,087 - b1821 b1821 0.66 +0.5 1,904,603 + rrmA b1822 0.40 +0.2 1,904,603 + rrmA b1822 0.40 +0.4 1,904,691 + rrmA b1822 0.51 -0.1 1,904,897 - rrmA b1822 0.77 -1.6 1,904,988 - rrmA b1822 0.88 +0.1 1,905,238 - +0.7 1,905,238 - +0.2
Or see this region's nucleotide sequence