Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intG and fliE are separated by 36 nucleotides fliE and fliF are separated by 214 nucleotides fliF and fliG overlap by 8 nucleotides fliG and fliH overlap by 8 nucleotides
b1936: intG - predicted defective phage integrase (pseudogene) (RefSeq), at 2,010,526 to 2,010,687
intG
b1937: fliE - flagellar hook-basal body protein FliE (NCBI), at 2,010,724 to 2,011,038
fliE
b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911
fliF
b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899
fliG
b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578
fliH
Position (kb)
2011
2012
2013 Strain fitness (log2 ratio)
-2
-1
0
1 at 2010.417 kb on - strand at 2010.449 kb on - strand at 2010.511 kb on - strand at 2010.520 kb on - strand at 2010.530 kb on - strand at 2010.530 kb on - strand at 2010.547 kb on + strand, within intG at 2010.555 kb on - strand, within intG at 2010.629 kb on - strand, within intG at 2010.673 kb on + strand at 2011.024 kb on + strand at 2011.097 kb on - strand at 2011.163 kb on - strand at 2011.228 kb on + strand at 2011.236 kb on - strand at 2011.367 kb on + strand at 2011.626 kb on - strand, within fliF at 2011.626 kb on - strand, within fliF at 2011.781 kb on + strand, within fliF at 2011.856 kb on - strand, within fliF at 2012.056 kb on - strand, within fliF at 2012.086 kb on + strand, within fliF at 2012.086 kb on + strand, within fliF at 2012.086 kb on + strand, within fliF at 2012.143 kb on - strand, within fliF at 2012.143 kb on - strand, within fliF at 2012.165 kb on - strand, within fliF at 2012.208 kb on - strand, within fliF at 2012.208 kb on - strand, within fliF at 2012.276 kb on + strand, within fliF at 2012.281 kb on + strand, within fliF at 2012.281 kb on + strand, within fliF at 2012.443 kb on - strand, within fliF at 2012.523 kb on - strand, within fliF at 2012.812 kb on - strand at 2012.830 kb on + strand at 2012.865 kb on + strand at 2012.873 kb on - strand at 2012.922 kb on + strand at 2012.979 kb on + strand at 2012.979 kb on + strand at 2013.108 kb on + strand, within fliG at 2013.108 kb on + strand, within fliG at 2013.149 kb on - strand, within fliG at 2013.444 kb on + strand, within fliG
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 2,010,417 - -1.1 2,010,449 - +0.3 2,010,511 - -0.8 2,010,520 - -2.3 2,010,530 - +0.3 2,010,530 - +0.5 2,010,547 + intG b1936 0.13 +1.5 2,010,555 - intG b1936 0.18 -0.3 2,010,629 - intG b1936 0.64 +0.3 2,010,673 + -0.6 2,011,024 + +0.2 2,011,097 - -1.1 2,011,163 - +0.1 2,011,228 + -0.7 2,011,236 - -0.9 2,011,367 + +0.3 2,011,626 - fliF b1938 0.22 +0.4 2,011,626 - fliF b1938 0.22 -0.5 2,011,781 + fliF b1938 0.32 -1.1 2,011,856 - fliF b1938 0.36 -0.1 2,012,056 - fliF b1938 0.48 -0.7 2,012,086 + fliF b1938 0.50 -0.5 2,012,086 + fliF b1938 0.50 -0.7 2,012,086 + fliF b1938 0.50 -0.3 2,012,143 - fliF b1938 0.54 -0.2 2,012,143 - fliF b1938 0.54 -1.8 2,012,165 - fliF b1938 0.55 -0.7 2,012,208 - fliF b1938 0.58 -1.0 2,012,208 - fliF b1938 0.58 -0.6 2,012,276 + fliF b1938 0.62 -0.2 2,012,281 + fliF b1938 0.62 -0.5 2,012,281 + fliF b1938 0.62 -0.0 2,012,443 - fliF b1938 0.72 -0.3 2,012,523 - fliF b1938 0.77 -0.5 2,012,812 - -0.5 2,012,830 + -0.1 2,012,865 + -0.2 2,012,873 - +0.1 2,012,922 + -0.3 2,012,979 + -0.2 2,012,979 + -0.8 2,013,108 + fliG b1939 0.20 -0.5 2,013,108 + fliG b1939 0.20 -0.6 2,013,149 - fliG b1939 0.25 -0.7 2,013,444 + fliG b1939 0.54 -0.4
Or see this region's nucleotide sequence