Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purR and ydhB overlap by 4 nucleotides ydhB and ydhC are separated by 112 nucleotides
b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893
purR
b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822
ydhB
b1660: ydhC - predicted transporter (NCBI), at 1,737,935 to 1,739,146
ydhC
Position (kb)
1736
1737
1738 Strain fitness (log2 ratio)
-1
0
1 at 1736.132 kb on + strand, within purR at 1736.147 kb on - strand, within purR at 1736.235 kb on - strand, within purR at 1736.243 kb on - strand, within purR at 1736.255 kb on + strand, within purR at 1736.255 kb on + strand, within purR at 1736.297 kb on + strand, within purR at 1736.319 kb on + strand, within purR at 1736.347 kb on - strand, within purR at 1736.506 kb on + strand, within purR at 1736.506 kb on + strand, within purR at 1736.688 kb on + strand, within purR at 1736.728 kb on + strand, within purR at 1736.728 kb on + strand, within purR at 1736.766 kb on - strand, within purR at 1736.820 kb on + strand at 1736.820 kb on - strand at 1736.843 kb on + strand at 1736.965 kb on + strand at 1737.011 kb on + strand, within ydhB at 1737.229 kb on - strand, within ydhB at 1737.332 kb on + strand, within ydhB at 1737.436 kb on - strand at 1737.689 kb on + strand, within ydhB at 1737.719 kb on + strand, within ydhB at 1738.071 kb on + strand, within ydhC at 1738.195 kb on - strand, within ydhC at 1738.195 kb on - strand, within ydhC at 1738.203 kb on + strand, within ydhC at 1738.203 kb on + strand, within ydhC at 1738.723 kb on - strand, within ydhC at 1738.723 kb on - strand, within ydhC
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 1,736,132 + purR b1658 0.26 -1.0 1,736,147 - purR b1658 0.27 -0.5 1,736,235 - purR b1658 0.36 -0.0 1,736,243 - purR b1658 0.37 -0.5 1,736,255 + purR b1658 0.38 +0.6 1,736,255 + purR b1658 0.38 -1.5 1,736,297 + purR b1658 0.42 +0.2 1,736,319 + purR b1658 0.44 -0.4 1,736,347 - purR b1658 0.47 -0.7 1,736,506 + purR b1658 0.62 -0.7 1,736,506 + purR b1658 0.62 -1.0 1,736,688 + purR b1658 0.80 -0.3 1,736,728 + purR b1658 0.84 -0.8 1,736,728 + purR b1658 0.84 +0.8 1,736,766 - purR b1658 0.88 -1.0 1,736,820 + -0.2 1,736,820 - +0.4 1,736,843 + -0.3 1,736,965 + +0.8 1,737,011 + ydhB b1659 0.13 +1.2 1,737,229 - ydhB b1659 0.36 -0.1 1,737,332 + ydhB b1659 0.47 -0.2 1,737,436 - -1.2 1,737,689 + ydhB b1659 0.86 -1.1 1,737,719 + ydhB b1659 0.89 +0.3 1,738,071 + ydhC b1660 0.11 -0.1 1,738,195 - ydhC b1660 0.21 +0.4 1,738,195 - ydhC b1660 0.21 +0.3 1,738,203 + ydhC b1660 0.22 +0.1 1,738,203 + ydhC b1660 0.22 +0.8 1,738,723 - ydhC b1660 0.65 +0.4 1,738,723 - ydhC b1660 0.65 +0.5
Or see this region's nucleotide sequence