Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmC and holD are separated by 102 nucleotides holD and rimI overlap by 32 nucleotides rimI and yjjG are separated by 14 nucleotides yjjG and prfC are separated by 90 nucleotides
b4371: rsmC - 16S ribosomal RNA m2G1207 methyltransferase (NCBI), at 4,604,692 to 4,605,723
rsmC
b4372: holD - DNA polymerase III subunit psi (NCBI), at 4,605,826 to 4,606,239
holD
b4373: rimI - ribosomal-protein-alanine N-acetyltransferase (NCBI), at 4,606,208 to 4,606,654
rimI
b4374: yjjG - nucleotidase (NCBI), at 4,606,669 to 4,607,346
yjjG
b4375: prfC - peptide chain release factor 3 (NCBI), at 4,607,437 to 4,609,026
prfC
Position (kb)
4606
4607 Strain fitness (log2 ratio)
-2
-1
0
1 at 4605.238 kb on + strand, within rsmC at 4605.238 kb on + strand, within rsmC at 4605.298 kb on - strand, within rsmC at 4605.304 kb on + strand, within rsmC at 4605.407 kb on + strand, within rsmC at 4605.453 kb on - strand, within rsmC at 4605.484 kb on - strand, within rsmC at 4605.484 kb on - strand, within rsmC at 4605.533 kb on - strand, within rsmC at 4605.564 kb on - strand, within rsmC at 4605.579 kb on - strand, within rsmC at 4605.579 kb on - strand, within rsmC at 4605.610 kb on + strand, within rsmC at 4605.662 kb on - strand at 4605.696 kb on - strand at 4605.696 kb on - strand at 4605.740 kb on - strand at 4605.783 kb on - strand at 4605.858 kb on + strand at 4606.288 kb on - strand, within rimI at 4606.288 kb on - strand, within rimI at 4606.309 kb on + strand, within rimI at 4606.309 kb on + strand, within rimI at 4606.385 kb on - strand, within rimI at 4606.611 kb on + strand at 4606.636 kb on - strand at 4606.652 kb on - strand at 4606.655 kb on - strand at 4606.668 kb on + strand at 4606.724 kb on + strand at 4606.724 kb on + strand at 4606.733 kb on + strand at 4606.796 kb on - strand, within yjjG at 4606.813 kb on + strand, within yjjG at 4606.813 kb on + strand, within yjjG at 4606.852 kb on - strand, within yjjG at 4606.855 kb on + strand, within yjjG at 4606.855 kb on + strand, within yjjG at 4606.860 kb on - strand, within yjjG at 4606.860 kb on - strand, within yjjG at 4606.877 kb on - strand, within yjjG at 4606.880 kb on + strand, within yjjG at 4606.880 kb on + strand, within yjjG at 4607.019 kb on - strand, within yjjG at 4607.038 kb on + strand, within yjjG at 4607.043 kb on + strand, within yjjG at 4607.252 kb on - strand, within yjjG at 4607.253 kb on - strand, within yjjG at 4607.253 kb on - strand, within yjjG at 4607.292 kb on + strand at 4607.292 kb on + strand at 4607.295 kb on - strand at 4607.295 kb on - strand at 4607.320 kb on - strand at 4607.320 kb on - strand at 4607.366 kb on - strand at 4607.416 kb on + strand at 4607.416 kb on + strand at 4607.460 kb on - strand at 4607.517 kb on - strand at 4607.517 kb on - strand at 4607.548 kb on - strand at 4607.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 4,605,238 + rsmC b4371 0.53 +0.8 4,605,238 + rsmC b4371 0.53 +0.1 4,605,298 - rsmC b4371 0.59 +0.6 4,605,304 + rsmC b4371 0.59 +0.2 4,605,407 + rsmC b4371 0.69 -1.8 4,605,453 - rsmC b4371 0.74 +0.8 4,605,484 - rsmC b4371 0.77 +0.3 4,605,484 - rsmC b4371 0.77 -0.1 4,605,533 - rsmC b4371 0.81 +0.3 4,605,564 - rsmC b4371 0.84 +0.0 4,605,579 - rsmC b4371 0.86 +0.1 4,605,579 - rsmC b4371 0.86 +0.1 4,605,610 + rsmC b4371 0.89 +0.1 4,605,662 - +0.2 4,605,696 - +1.1 4,605,696 - +0.5 4,605,740 - -0.3 4,605,783 - +0.0 4,605,858 + -0.0 4,606,288 - rimI b4373 0.18 -0.6 4,606,288 - rimI b4373 0.18 -0.7 4,606,309 + rimI b4373 0.23 -0.5 4,606,309 + rimI b4373 0.23 -0.3 4,606,385 - rimI b4373 0.40 +0.0 4,606,611 + +0.1 4,606,636 - +0.4 4,606,652 - +0.2 4,606,655 - -1.2 4,606,668 + -1.0 4,606,724 + +0.3 4,606,724 + -0.1 4,606,733 + +0.4 4,606,796 - yjjG b4374 0.19 +0.3 4,606,813 + yjjG b4374 0.21 -0.8 4,606,813 + yjjG b4374 0.21 +0.1 4,606,852 - yjjG b4374 0.27 -0.6 4,606,855 + yjjG b4374 0.27 +0.7 4,606,855 + yjjG b4374 0.27 +0.1 4,606,860 - yjjG b4374 0.28 -0.4 4,606,860 - yjjG b4374 0.28 +0.1 4,606,877 - yjjG b4374 0.31 +0.6 4,606,880 + yjjG b4374 0.31 -0.2 4,606,880 + yjjG b4374 0.31 +0.4 4,607,019 - yjjG b4374 0.52 -0.2 4,607,038 + yjjG b4374 0.54 +0.7 4,607,043 + yjjG b4374 0.55 +0.2 4,607,252 - yjjG b4374 0.86 -0.5 4,607,253 - yjjG b4374 0.86 +0.4 4,607,253 - yjjG b4374 0.86 +0.1 4,607,292 + +0.7 4,607,292 + +0.6 4,607,295 - -0.1 4,607,295 - +0.6 4,607,320 - +0.4 4,607,320 - +0.6 4,607,366 - -1.2 4,607,416 + +0.7 4,607,416 + -0.8 4,607,460 - +0.3 4,607,517 - -1.2 4,607,517 - +0.5 4,607,548 - -1.4 4,607,562 + -2.5
Or see this region's nucleotide sequence