Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoU and pstB are separated by 14 nucleotides pstB and pstA are separated by 182 nucleotides
b3724: phoU - negative regulator of PhoR/PhoB two-component regulator (NCBI), at 3,904,876 to 3,905,601
phoU
b3725: pstB - phosphate transporter subunit (NCBI), at 3,905,616 to 3,906,389
pstB
b3726: pstA - phosphate transporter subunit (NCBI), at 3,906,572 to 3,907,462
pstA
Position (kb)
3905
3906
3907 Strain fitness (log2 ratio)
-1
0
1 at 3904.668 kb on + strand at 3904.668 kb on + strand at 3904.798 kb on - strand at 3904.861 kb on - strand at 3904.868 kb on - strand at 3904.896 kb on - strand at 3904.930 kb on - strand at 3905.604 kb on - strand at 3905.604 kb on - strand at 3905.604 kb on - strand at 3905.608 kb on + strand at 3905.665 kb on - strand at 3905.700 kb on + strand, within pstB at 3906.128 kb on + strand, within pstB at 3906.136 kb on - strand, within pstB at 3906.381 kb on - strand at 3906.387 kb on - strand at 3906.468 kb on - strand at 3906.483 kb on + strand at 3906.493 kb on - strand at 3906.493 kb on - strand at 3906.524 kb on - strand at 3906.526 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.574 kb on - strand at 3906.574 kb on - strand at 3906.579 kb on + strand at 3906.587 kb on - strand at 3906.587 kb on - strand at 3906.614 kb on + strand at 3906.642 kb on - strand at 3906.642 kb on - strand at 3906.644 kb on - strand at 3906.779 kb on - strand, within pstA at 3906.835 kb on - strand, within pstA at 3906.836 kb on - strand, within pstA at 3906.888 kb on - strand, within pstA at 3906.905 kb on - strand, within pstA at 3906.918 kb on + strand, within pstA at 3906.928 kb on - strand, within pstA at 3906.951 kb on - strand, within pstA at 3906.992 kb on - strand, within pstA at 3907.077 kb on - strand, within pstA at 3907.084 kb on - strand, within pstA at 3907.084 kb on - strand, within pstA at 3907.089 kb on - strand, within pstA at 3907.199 kb on - strand, within pstA at 3907.218 kb on - strand, within pstA at 3907.218 kb on - strand, within pstA at 3907.236 kb on - strand, within pstA at 3907.236 kb on - strand, within pstA at 3907.337 kb on - strand, within pstA
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 3,904,668 + -1.0 3,904,668 + +1.1 3,904,798 - +0.1 3,904,861 - +0.7 3,904,868 - -0.0 3,904,896 - +0.3 3,904,930 - -0.6 3,905,604 - -0.4 3,905,604 - -0.7 3,905,604 - -0.0 3,905,608 + -0.4 3,905,665 - -0.3 3,905,700 + pstB b3725 0.11 -0.9 3,906,128 + pstB b3725 0.66 +0.3 3,906,136 - pstB b3725 0.67 +0.3 3,906,381 - -0.2 3,906,387 - -1.0 3,906,468 - +0.3 3,906,483 + -1.5 3,906,493 - -0.1 3,906,493 - -0.2 3,906,524 - -0.2 3,906,526 + -0.3 3,906,538 + +0.6 3,906,538 + -0.1 3,906,538 + -0.6 3,906,538 + +0.6 3,906,546 - +0.8 3,906,546 - +0.0 3,906,546 - -0.5 3,906,546 - +0.3 3,906,546 - +0.9 3,906,574 - +0.3 3,906,574 - -0.5 3,906,579 + -0.3 3,906,587 - +0.1 3,906,587 - -0.2 3,906,614 + -0.1 3,906,642 - -1.3 3,906,642 - -0.1 3,906,644 - -0.5 3,906,779 - pstA b3726 0.23 +1.0 3,906,835 - pstA b3726 0.30 -0.3 3,906,836 - pstA b3726 0.30 +0.1 3,906,888 - pstA b3726 0.35 -0.6 3,906,905 - pstA b3726 0.37 +0.0 3,906,918 + pstA b3726 0.39 +0.1 3,906,928 - pstA b3726 0.40 -1.3 3,906,951 - pstA b3726 0.43 +0.6 3,906,992 - pstA b3726 0.47 +0.7 3,907,077 - pstA b3726 0.57 -0.9 3,907,084 - pstA b3726 0.57 +0.2 3,907,084 - pstA b3726 0.57 +0.2 3,907,089 - pstA b3726 0.58 +0.0 3,907,199 - pstA b3726 0.70 +0.3 3,907,218 - pstA b3726 0.73 -1.1 3,907,218 - pstA b3726 0.73 +0.5 3,907,236 - pstA b3726 0.75 -0.0 3,907,236 - pstA b3726 0.75 +0.6 3,907,337 - pstA b3726 0.86 -0.7
Or see this region's nucleotide sequence