Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt qseC and ygiZ are separated by 45 nucleotides ygiZ and mdaB are separated by 318 nucleotides mdaB and ygiN are separated by 30 nucleotides
b3026: qseC - sensory histidine kinase in two-component regulatory system with QseB (NCBI), at 3,168,506 to 3,169,855
qseC
b3027: ygiZ - conserved inner membrane protein (NCBI), at 3,169,901 to 3,170,233
ygiZ
b3028: mdaB - NADPH quinone reductase (NCBI), at 3,170,552 to 3,171,133
mdaB
b3029: ygiN - quinol monooxygenase (NCBI), at 3,171,164 to 3,171,478
ygiN
Position (kb)
3169
3170
3171 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3168.909 kb on + strand, within qseC at 3168.943 kb on - strand, within qseC at 3169.046 kb on + strand, within qseC at 3169.087 kb on + strand, within qseC at 3169.161 kb on + strand, within qseC at 3169.194 kb on + strand, within qseC at 3169.200 kb on - strand, within qseC at 3169.246 kb on - strand, within qseC at 3169.247 kb on - strand, within qseC at 3169.265 kb on - strand, within qseC at 3169.341 kb on - strand, within qseC at 3169.397 kb on + strand, within qseC at 3169.397 kb on + strand, within qseC at 3169.419 kb on - strand, within qseC at 3169.477 kb on + strand, within qseC at 3169.485 kb on - strand, within qseC at 3169.521 kb on - strand, within qseC at 3169.528 kb on - strand, within qseC at 3169.537 kb on + strand, within qseC at 3169.620 kb on + strand, within qseC at 3169.620 kb on + strand, within qseC at 3169.625 kb on + strand, within qseC at 3169.625 kb on + strand, within qseC at 3169.677 kb on + strand, within qseC at 3169.752 kb on + strand at 3169.752 kb on + strand at 3169.800 kb on + strand at 3169.825 kb on + strand at 3169.825 kb on + strand at 3169.830 kb on + strand at 3169.830 kb on + strand at 3169.840 kb on + strand at 3169.841 kb on + strand at 3169.849 kb on - strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.863 kb on + strand at 3169.871 kb on - strand at 3169.874 kb on + strand at 3169.874 kb on + strand at 3169.888 kb on + strand at 3169.911 kb on + strand at 3169.911 kb on + strand at 3169.912 kb on + strand at 3169.936 kb on + strand, within ygiZ at 3169.951 kb on + strand, within ygiZ at 3169.985 kb on + strand, within ygiZ at 3170.288 kb on - strand at 3170.356 kb on - strand at 3170.390 kb on - strand at 3170.395 kb on - strand at 3170.422 kb on - strand at 3170.422 kb on - strand at 3170.430 kb on - strand at 3170.438 kb on + strand at 3170.438 kb on + strand at 3170.446 kb on - strand at 3170.446 kb on - strand at 3170.521 kb on - strand at 3170.521 kb on - strand at 3170.521 kb on - strand at 3170.521 kb on - strand at 3170.596 kb on + strand at 3170.596 kb on + strand at 3170.601 kb on + strand at 3170.748 kb on - strand, within mdaB at 3170.748 kb on - strand, within mdaB at 3170.750 kb on + strand, within mdaB at 3170.761 kb on + strand, within mdaB at 3170.781 kb on - strand, within mdaB at 3170.828 kb on + strand, within mdaB at 3170.838 kb on - strand, within mdaB at 3170.894 kb on - strand, within mdaB at 3170.894 kb on - strand, within mdaB at 3170.978 kb on + strand, within mdaB at 3170.978 kb on + strand, within mdaB at 3170.986 kb on - strand, within mdaB at 3170.986 kb on - strand, within mdaB at 3170.988 kb on + strand, within mdaB at 3171.004 kb on + strand, within mdaB at 3171.098 kb on - strand at 3171.132 kb on + strand at 3171.132 kb on + strand at 3171.163 kb on - strand at 3171.209 kb on - strand, within ygiN at 3171.209 kb on - strand, within ygiN at 3171.223 kb on + strand, within ygiN
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 3,168,909 + qseC b3026 0.30 +0.5 3,168,943 - qseC b3026 0.32 +1.4 3,169,046 + qseC b3026 0.40 +0.2 3,169,087 + qseC b3026 0.43 -1.5 3,169,161 + qseC b3026 0.49 -0.6 3,169,194 + qseC b3026 0.51 -0.2 3,169,200 - qseC b3026 0.51 +1.1 3,169,246 - qseC b3026 0.55 -2.1 3,169,247 - qseC b3026 0.55 -1.1 3,169,265 - qseC b3026 0.56 +0.8 3,169,341 - qseC b3026 0.62 -0.0 3,169,397 + qseC b3026 0.66 +1.1 3,169,397 + qseC b3026 0.66 -0.6 3,169,419 - qseC b3026 0.68 +0.0 3,169,477 + qseC b3026 0.72 -1.7 3,169,485 - qseC b3026 0.73 +1.4 3,169,521 - qseC b3026 0.75 +0.1 3,169,528 - qseC b3026 0.76 +0.2 3,169,537 + qseC b3026 0.76 -0.9 3,169,620 + qseC b3026 0.83 +0.1 3,169,620 + qseC b3026 0.83 +1.2 3,169,625 + qseC b3026 0.83 -0.2 3,169,625 + qseC b3026 0.83 -0.6 3,169,677 + qseC b3026 0.87 -1.5 3,169,752 + -0.5 3,169,752 + -0.0 3,169,800 + -2.0 3,169,825 + -0.8 3,169,825 + -0.6 3,169,830 + +0.0 3,169,830 + -0.4 3,169,840 + +0.3 3,169,841 + -0.4 3,169,849 - +0.1 3,169,863 + -0.4 3,169,863 + +0.4 3,169,863 + -0.4 3,169,863 + +0.4 3,169,863 + +0.0 3,169,863 + +0.0 3,169,863 + +0.3 3,169,863 + +0.2 3,169,863 + -0.1 3,169,871 - +2.4 3,169,874 + -1.4 3,169,874 + -0.8 3,169,888 + -1.9 3,169,911 + +1.8 3,169,911 + +0.4 3,169,912 + +0.4 3,169,936 + ygiZ b3027 0.11 +0.4 3,169,951 + ygiZ b3027 0.15 +1.4 3,169,985 + ygiZ b3027 0.25 +1.4 3,170,288 - -0.2 3,170,356 - +1.2 3,170,390 - -0.4 3,170,395 - -0.2 3,170,422 - -0.3 3,170,422 - +0.3 3,170,430 - +0.5 3,170,438 + +0.8 3,170,438 + +0.8 3,170,446 - +0.4 3,170,446 - -0.1 3,170,521 - +2.4 3,170,521 - -0.4 3,170,521 - -0.5 3,170,521 - +0.7 3,170,596 + -0.1 3,170,596 + +0.2 3,170,601 + +0.1 3,170,748 - mdaB b3028 0.34 +1.2 3,170,748 - mdaB b3028 0.34 +0.4 3,170,750 + mdaB b3028 0.34 +0.2 3,170,761 + mdaB b3028 0.36 -2.0 3,170,781 - mdaB b3028 0.39 +0.1 3,170,828 + mdaB b3028 0.47 +0.1 3,170,838 - mdaB b3028 0.49 -0.2 3,170,894 - mdaB b3028 0.59 -0.0 3,170,894 - mdaB b3028 0.59 +0.1 3,170,978 + mdaB b3028 0.73 +0.0 3,170,978 + mdaB b3028 0.73 -0.2 3,170,986 - mdaB b3028 0.75 +0.7 3,170,986 - mdaB b3028 0.75 -0.5 3,170,988 + mdaB b3028 0.75 +0.3 3,171,004 + mdaB b3028 0.78 -0.1 3,171,098 - +0.3 3,171,132 + +0.1 3,171,132 + +0.2 3,171,163 - +1.0 3,171,209 - ygiN b3029 0.14 +0.3 3,171,209 - ygiN b3029 0.14 -0.1 3,171,223 + ygiN b3029 0.19 +1.3
Or see this region's nucleotide sequence