Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsP and nudH are separated by 12 nucleotides nudH and mutH are separated by 684 nucleotides
b2829: ptsP - fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI), at 2,964,210 to 2,966,456
ptsP
b2830: nudH - dinucleoside polyphosphate hydrolase (NCBI), at 2,966,469 to 2,966,999
nudH
b2831: mutH - DNA mismatch repair protein (NCBI), at 2,967,684 to 2,968,373
mutH
Position (kb)
2966
2967 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2965.575 kb on - strand, within ptsP at 2965.575 kb on - strand, within ptsP at 2965.860 kb on + strand, within ptsP at 2965.876 kb on + strand, within ptsP at 2965.910 kb on - strand, within ptsP at 2965.937 kb on - strand, within ptsP at 2965.937 kb on - strand, within ptsP at 2965.968 kb on + strand, within ptsP at 2965.971 kb on + strand, within ptsP at 2966.002 kb on + strand, within ptsP at 2966.055 kb on - strand, within ptsP at 2966.063 kb on + strand at 2966.063 kb on + strand, within ptsP at 2966.064 kb on + strand, within ptsP at 2966.089 kb on - strand, within ptsP at 2966.089 kb on - strand, within ptsP at 2966.167 kb on - strand, within ptsP at 2966.258 kb on + strand at 2966.346 kb on + strand at 2966.346 kb on + strand at 2966.346 kb on - strand at 2966.400 kb on + strand at 2966.459 kb on - strand at 2966.459 kb on - strand at 2966.465 kb on + strand at 2966.467 kb on + strand at 2966.467 kb on + strand at 2966.474 kb on - strand at 2966.484 kb on - strand at 2966.484 kb on - strand at 2966.532 kb on + strand, within nudH at 2966.565 kb on + strand, within nudH at 2966.645 kb on + strand, within nudH at 2966.645 kb on + strand, within nudH at 2966.722 kb on + strand, within nudH at 2967.071 kb on + strand at 2967.078 kb on + strand at 2967.078 kb on + strand at 2967.640 kb on - strand at 2967.694 kb on - strand at 2967.728 kb on + strand at 2967.768 kb on - strand, within mutH at 2967.768 kb on - strand, within mutH at 2967.994 kb on - strand, within mutH
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 2,965,575 - ptsP b2829 0.61 +0.5 2,965,575 - ptsP b2829 0.61 +1.0 2,965,860 + ptsP b2829 0.73 +0.6 2,965,876 + ptsP b2829 0.74 +0.2 2,965,910 - ptsP b2829 0.76 +1.4 2,965,937 - ptsP b2829 0.77 +0.5 2,965,937 - ptsP b2829 0.77 +1.3 2,965,968 + ptsP b2829 0.78 +0.3 2,965,971 + ptsP b2829 0.78 +0.6 2,966,002 + ptsP b2829 0.80 +0.4 2,966,055 - ptsP b2829 0.82 +0.3 2,966,063 + +2.0 2,966,063 + ptsP b2829 0.82 +0.5 2,966,064 + ptsP b2829 0.83 -0.1 2,966,089 - ptsP b2829 0.84 +0.2 2,966,089 - ptsP b2829 0.84 -0.2 2,966,167 - ptsP b2829 0.87 +0.4 2,966,258 + +0.4 2,966,346 + -0.2 2,966,346 + +0.5 2,966,346 - -0.2 2,966,400 + -0.0 2,966,459 - +0.5 2,966,459 - +0.3 2,966,465 + +0.1 2,966,467 + -0.9 2,966,467 + +0.4 2,966,474 - +0.3 2,966,484 - +0.0 2,966,484 - +0.4 2,966,532 + nudH b2830 0.12 +2.3 2,966,565 + nudH b2830 0.18 +0.3 2,966,645 + nudH b2830 0.33 -0.5 2,966,645 + nudH b2830 0.33 +0.0 2,966,722 + nudH b2830 0.48 -1.4 2,967,071 + +0.2 2,967,078 + +0.5 2,967,078 + +0.4 2,967,640 - -0.2 2,967,694 - +3.3 2,967,728 + -0.8 2,967,768 - mutH b2831 0.12 +0.2 2,967,768 - mutH b2831 0.12 -0.1 2,967,994 - mutH b2831 0.45 -0.6
Or see this region's nucleotide sequence