Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recO and era are separated by 11 nucleotides era and rnc overlap by 4 nucleotides rnc and lepB are separated by 271 nucleotides
b2565: recO - DNA repair protein RecO (NCBI), at 2,699,763 to 2,700,491
recO
b2566: era - GTP-binding protein Era (NCBI), at 2,700,503 to 2,701,408
era
b2567: rnc - ribonuclease III (NCBI), at 2,701,405 to 2,702,085
rnc
b2568: lepB - leader peptidase (signal peptidase I) (NCBI), at 2,702,357 to 2,703,331
lepB
Position (kb)
2701
2702
2703 Strain fitness (log2 ratio)
-2
-1
0
1 at 2700.467 kb on - strand at 2701.692 kb on - strand, within rnc at 2702.009 kb on - strand, within rnc at 2702.100 kb on - strand at 2702.134 kb on - strand at 2702.144 kb on - strand at 2702.144 kb on - strand at 2702.147 kb on - strand at 2702.147 kb on - strand at 2702.164 kb on - strand at 2702.164 kb on - strand at 2702.191 kb on - strand at 2702.191 kb on - strand at 2702.191 kb on - strand at 2702.229 kb on - strand at 2702.303 kb on + strand at 2702.368 kb on + strand at 2702.368 kb on + strand at 2702.439 kb on - strand at 2702.448 kb on - strand at 2702.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 2,700,467 - -0.4 2,701,692 - rnc b2567 0.42 -1.2 2,702,009 - rnc b2567 0.89 -0.8 2,702,100 - -0.4 2,702,134 - -0.1 2,702,144 - -0.2 2,702,144 - -0.6 2,702,147 - +0.5 2,702,147 - +0.6 2,702,164 - -0.1 2,702,164 - -0.5 2,702,191 - -0.7 2,702,191 - -0.7 2,702,191 - +0.1 2,702,229 - -0.9 2,702,303 + +0.2 2,702,368 + +1.4 2,702,368 + +0.7 2,702,439 - -1.9 2,702,448 - +0.0 2,702,448 - -0.8
Or see this region's nucleotide sequence