Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoC and nuoB are separated by 105 nucleotides nuoB and nuoA are separated by 15 nucleotides nuoA and lrhA are separated by 630 nucleotides
b2286: nuoC - NADH:ubiquinone oxidoreductase, chain C,D (RefSeq), at 2,400,077 to 2,401,867
nuoC
b2287: nuoB - NADH dehydrogenase subunit B (NCBI), at 2,401,973 to 2,402,635
nuoB
b2288: nuoA - NADH dehydrogenase I chain A (VIMSS), at 2,402,651 to 2,403,094
nuoA
b2289: lrhA - DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI), at 2,403,725 to 2,404,663
lrhA
Position (kb)
2402
2403
2404 Strain fitness (log2 ratio)
-2
-1
0
1 at 2401.665 kb on + strand, within nuoC at 2401.730 kb on - strand at 2401.730 kb on - strand at 2401.732 kb on - strand at 2401.732 kb on - strand at 2401.762 kb on - strand at 2401.767 kb on - strand at 2402.000 kb on + strand at 2402.085 kb on - strand, within nuoB at 2402.169 kb on + strand, within nuoB at 2402.169 kb on + strand, within nuoB at 2402.179 kb on - strand, within nuoB at 2402.212 kb on - strand, within nuoB at 2402.284 kb on + strand, within nuoB at 2402.378 kb on - strand, within nuoB at 2402.386 kb on - strand, within nuoB at 2402.386 kb on - strand, within nuoB at 2402.434 kb on + strand, within nuoB at 2402.434 kb on + strand, within nuoB at 2402.583 kb on + strand at 2402.621 kb on - strand at 2402.624 kb on + strand at 2402.662 kb on + strand at 2402.662 kb on + strand at 2402.665 kb on + strand at 2402.665 kb on + strand at 2402.694 kb on - strand at 2402.723 kb on + strand, within nuoA at 2402.723 kb on + strand, within nuoA at 2402.745 kb on + strand at 2402.805 kb on - strand, within nuoA at 2402.805 kb on - strand, within nuoA at 2402.819 kb on + strand, within nuoA at 2402.894 kb on - strand, within nuoA at 2402.963 kb on - strand, within nuoA at 2403.064 kb on - strand at 2403.070 kb on + strand at 2403.070 kb on + strand at 2403.078 kb on - strand at 2403.081 kb on + strand at 2403.126 kb on + strand at 2403.126 kb on + strand at 2403.368 kb on - strand at 2403.368 kb on - strand at 2403.473 kb on + strand at 2403.785 kb on + strand at 2403.841 kb on + strand, within lrhA at 2403.916 kb on + strand, within lrhA at 2404.060 kb on - strand at 2404.060 kb on - strand, within lrhA
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 2,401,665 + nuoC b2286 0.89 -1.8 2,401,730 - -1.5 2,401,730 - -0.5 2,401,732 - +0.3 2,401,732 - -0.7 2,401,762 - -0.5 2,401,767 - +0.2 2,402,000 + -0.6 2,402,085 - nuoB b2287 0.17 -0.5 2,402,169 + nuoB b2287 0.30 -0.5 2,402,169 + nuoB b2287 0.30 +0.6 2,402,179 - nuoB b2287 0.31 -0.2 2,402,212 - nuoB b2287 0.36 -0.2 2,402,284 + nuoB b2287 0.47 -1.8 2,402,378 - nuoB b2287 0.61 -0.1 2,402,386 - nuoB b2287 0.62 -0.5 2,402,386 - nuoB b2287 0.62 -0.9 2,402,434 + nuoB b2287 0.70 -0.9 2,402,434 + nuoB b2287 0.70 -0.5 2,402,583 + -2.2 2,402,621 - -0.2 2,402,624 + -0.6 2,402,662 + -0.3 2,402,662 + -1.3 2,402,665 + -0.1 2,402,665 + +0.3 2,402,694 - -0.1 2,402,723 + nuoA b2288 0.16 -2.5 2,402,723 + nuoA b2288 0.16 -0.9 2,402,745 + -0.7 2,402,805 - nuoA b2288 0.35 -1.2 2,402,805 - nuoA b2288 0.35 -0.1 2,402,819 + nuoA b2288 0.38 -1.3 2,402,894 - nuoA b2288 0.55 -2.3 2,402,963 - nuoA b2288 0.70 -0.7 2,403,064 - -2.3 2,403,070 + -1.1 2,403,070 + +0.4 2,403,078 - -0.7 2,403,081 + -1.3 2,403,126 + +0.2 2,403,126 + -0.6 2,403,368 - -0.0 2,403,368 - +0.1 2,403,473 + +1.0 2,403,785 + -1.5 2,403,841 + lrhA b2289 0.12 +0.1 2,403,916 + lrhA b2289 0.20 -0.2 2,404,060 - +0.4 2,404,060 - lrhA b2289 0.36 +0.5
Or see this region's nucleotide sequence