Strain Fitness in Escherichia coli BW25113 around b2277

Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
2,388,545	+	nuoN	b2276	0.33	-1.2
2,388,665	-	nuoN	b2276	0.41	-0.2
2,388,762	-	nuoN	b2276	0.47	-0.9
2,388,773	-	nuoN	b2276	0.48	-1.2
2,388,835	+	nuoN	b2276	0.52	+1.1
2,388,999	-	nuoN	b2276	0.64	-1.8
2,389,002	+	nuoN	b2276	0.64	-0.3
2,389,005	-	nuoN	b2276	0.64	-1.8
2,389,005	-	nuoN	b2276	0.64	-1.1
2,389,007	+	nuoN	b2276	0.64	-1.4
2,389,258	-	nuoN	b2276	0.81	-0.5
2,389,295	-	nuoN	b2276	0.84	-0.4
2,389,306	-	nuoN	b2276	0.85	-0.7
2,389,306	-	nuoN	b2276	0.85	-0.7
2,389,442	+				-1.5
2,389,461	-				-1.9
2,389,517	+				+0.6
2,389,539	+				-1.6
2,389,787	-	nuoM	b2277	0.17	-0.3
2,389,817	-	nuoM	b2277	0.18	-0.3
2,389,843	-	nuoM	b2277	0.20	-0.9
2,389,938	-	nuoM	b2277	0.26	-1.2
2,389,972	-	nuoM	b2277	0.29	+0.1
2,390,000	-	nuoM	b2277	0.30	-1.0
2,390,028	-	nuoM	b2277	0.32	-0.9
2,390,028	-	nuoM	b2277	0.32	-1.1
2,390,151	-	nuoM	b2277	0.40	-0.8
2,390,151	-	nuoM	b2277	0.40	-1.2
2,390,162	+	nuoM	b2277	0.41	-1.2
2,390,162	+	nuoM	b2277	0.41	-0.5
2,390,162	+	nuoM	b2277	0.41	-0.7
2,390,170	-	nuoM	b2277	0.42	-0.5
2,390,211	+	nuoM	b2277	0.44	+0.0
2,390,230	-	nuoM	b2277	0.45	-0.0
2,390,312	+	nuoM	b2277	0.51	-1.4
2,390,341	+	nuoM	b2277	0.53	-0.6
2,390,383	-	nuoM	b2277	0.55	-0.7
2,390,411	+	nuoM	b2277	0.57	-0.7
2,390,426	-	nuoM	b2277	0.58	-0.4
2,390,454	-	nuoM	b2277	0.60	-2.1
2,390,511	-	nuoM	b2277	0.64	-0.7
2,390,516	-	nuoM	b2277	0.64	+1.3
2,390,516	-	nuoM	b2277	0.64	-0.4
2,390,522	-				+0.1
2,390,522	-	nuoM	b2277	0.65	-0.4
2,390,530	+	nuoM	b2277	0.65	+0.8
2,390,575	-	nuoM	b2277	0.68	+0.1
2,390,591	-	nuoM	b2277	0.69	-0.2
2,390,591	-	nuoM	b2277	0.69	-1.0
2,390,596	-	nuoM	b2277	0.69	-0.3
2,390,598	-	nuoM	b2277	0.70	-0.3
2,390,886	-	nuoM	b2277	0.88	-1.6
2,390,886	-	nuoM	b2277	0.88	+0.9
2,390,912	-				-1.3
2,390,948	+				-1.2
2,390,972	+				-1.8
2,391,053	+				-0.6
2,391,068	-				-1.1
2,391,102	+				+0.3
2,391,150	+				-0.2
2,391,153	+				-2.0
2,391,197	+				-2.2
2,391,238	-				-1.5
2,391,245	-				-0.4
2,391,248	+				-0.7
2,391,259	+				-0.7
2,391,278	-				+0.1
2,391,281	-				+0.5
2,391,281	-				-0.0
2,391,317	+				-0.7
2,391,372	-				-0.0
2,391,414	+				-0.8
2,391,414	+	nuoL	b2278	0.10	+1.7
2,391,419	+	nuoL	b2278	0.10	-0.1
2,391,439	-	nuoL	b2278	0.12	-0.2
2,391,505	-	nuoL	b2278	0.15	-0.8
2,391,505	-	nuoL	b2278	0.15	-0.2
2,391,517	+	nuoL	b2278	0.16	+0.5
2,391,567	-	nuoL	b2278	0.18	+0.9
2,391,785	+	nuoL	b2278	0.30	-2.5
2,391,795	+	nuoL	b2278	0.31	+0.6
2,391,809	+	nuoL	b2278	0.32	-2.0
2,391,809	+	nuoL	b2278	0.32	-1.3
2,391,921	-	nuoL	b2278	0.38	-0.8
2,391,985	-	nuoL	b2278	0.41	-0.7
2,392,049	-	nuoL	b2278	0.45	-1.5