Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and eda are separated by 55 nucleotides eda and edd are separated by 36 nucleotides
b1849: purT - phosphoribosylglycinamide formyltransferase 2 (NCBI), at 1,928,905 to 1,930,083
purT
b1850: eda - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI), at 1,930,139 to 1,930,780
eda
b1851: edd - phosphogluconate dehydratase (NCBI), at 1,930,817 to 1,932,628
edd
Position (kb)
1930
1931 Strain fitness (log2 ratio)
-2
-1
0
1 at 1929.150 kb on - strand, within purT at 1929.362 kb on + strand, within purT at 1929.362 kb on + strand, within purT at 1929.640 kb on - strand, within purT at 1929.640 kb on - strand, within purT at 1929.683 kb on + strand, within purT at 1929.683 kb on + strand, within purT at 1929.759 kb on - strand, within purT at 1929.800 kb on + strand, within purT at 1930.003 kb on + strand at 1930.263 kb on + strand, within eda at 1930.411 kb on - strand, within eda at 1930.626 kb on - strand, within eda at 1930.626 kb on - strand, within eda at 1930.833 kb on + strand at 1930.841 kb on - strand at 1930.843 kb on + strand at 1930.843 kb on + strand at 1930.988 kb on + strand at 1931.044 kb on - strand, within edd at 1931.065 kb on - strand, within edd at 1931.065 kb on - strand, within edd at 1931.095 kb on + strand, within edd at 1931.142 kb on + strand, within edd at 1931.353 kb on - strand, within edd at 1931.392 kb on + strand, within edd at 1931.484 kb on - strand, within edd at 1931.549 kb on - strand, within edd at 1931.549 kb on - strand, within edd at 1931.566 kb on - strand, within edd at 1931.566 kb on - strand, within edd at 1931.591 kb on - strand, within edd
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 1,929,150 - purT b1849 0.21 +0.1 1,929,362 + purT b1849 0.39 -0.4 1,929,362 + purT b1849 0.39 -0.2 1,929,640 - purT b1849 0.62 -0.3 1,929,640 - purT b1849 0.62 +0.2 1,929,683 + purT b1849 0.66 +0.1 1,929,683 + purT b1849 0.66 +0.4 1,929,759 - purT b1849 0.72 +0.3 1,929,800 + purT b1849 0.76 +0.4 1,930,003 + -0.8 1,930,263 + eda b1850 0.19 -1.0 1,930,411 - eda b1850 0.42 -1.0 1,930,626 - eda b1850 0.76 -2.1 1,930,626 - eda b1850 0.76 +1.3 1,930,833 + -0.1 1,930,841 - +0.3 1,930,843 + +0.3 1,930,843 + +0.6 1,930,988 + +0.1 1,931,044 - edd b1851 0.13 -0.0 1,931,065 - edd b1851 0.14 +0.4 1,931,065 - edd b1851 0.14 +0.2 1,931,095 + edd b1851 0.15 +0.0 1,931,142 + edd b1851 0.18 -0.2 1,931,353 - edd b1851 0.30 +0.0 1,931,392 + edd b1851 0.32 +0.2 1,931,484 - edd b1851 0.37 +0.4 1,931,549 - edd b1851 0.40 -0.1 1,931,549 - edd b1851 0.40 -0.1 1,931,566 - edd b1851 0.41 -0.2 1,931,566 - edd b1851 0.41 +0.6 1,931,591 - edd b1851 0.43 -0.1
Or see this region's nucleotide sequence