Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insF-4 and insE overlap by 4 nucleotides insE and ymdE are separated by 67 nucleotides ymdE and ycdU overlap by 4 nucleotides
b1026: insF-4 - IS3 element protein InsF (NCBI), at 1,093,498 to 1,094,364
insF-4
b1027: insE - IS3 element protein InsE (RefSeq), at 1,094,361 to 1,094,660
insE
b1028: ymdE - orf, hypothetical protein (VIMSS), at 1,094,728 to 1,095,069
ymdE
b1029: ycdU - predicted inner membrane protein (NCBI), at 1,095,066 to 1,096,052
ycdU
Position (kb)
1094
1095
1096 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1094.690 kb on + strand at 1094.690 kb on + strand at 1094.710 kb on + strand at 1094.710 kb on + strand at 1094.715 kb on + strand at 1094.728 kb on + strand at 1094.733 kb on + strand at 1094.815 kb on + strand, within ymdE at 1094.846 kb on - strand, within ymdE at 1094.880 kb on + strand, within ymdE at 1094.881 kb on + strand, within ymdE at 1094.888 kb on + strand, within ymdE at 1094.888 kb on + strand, within ymdE at 1094.888 kb on + strand, within ymdE at 1094.888 kb on + strand, within ymdE at 1094.913 kb on + strand, within ymdE at 1094.914 kb on + strand, within ymdE at 1094.921 kb on + strand, within ymdE at 1095.010 kb on + strand, within ymdE at 1095.010 kb on + strand, within ymdE at 1095.010 kb on + strand, within ymdE at 1095.020 kb on + strand, within ymdE at 1095.020 kb on + strand, within ymdE at 1095.020 kb on + strand, within ymdE at 1095.022 kb on + strand, within ymdE at 1095.022 kb on + strand, within ymdE at 1095.022 kb on + strand, within ymdE at 1095.022 kb on + strand, within ymdE at 1095.032 kb on + strand, within ymdE at 1095.402 kb on - strand, within ycdU at 1095.408 kb on - strand, within ycdU at 1095.442 kb on - strand, within ycdU at 1095.499 kb on + strand, within ycdU at 1095.566 kb on + strand, within ycdU at 1095.566 kb on + strand, within ycdU at 1095.567 kb on + strand, within ycdU at 1095.581 kb on + strand, within ycdU at 1095.597 kb on - strand, within ycdU at 1095.597 kb on - strand, within ycdU at 1095.612 kb on + strand, within ycdU at 1095.650 kb on + strand, within ycdU at 1095.650 kb on + strand, within ycdU at 1095.650 kb on + strand, within ycdU at 1095.652 kb on + strand, within ycdU at 1095.699 kb on + strand, within ycdU at 1095.701 kb on - strand, within ycdU at 1095.730 kb on + strand, within ycdU at 1095.730 kb on + strand, within ycdU at 1095.730 kb on + strand, within ycdU at 1095.740 kb on + strand, within ycdU at 1095.782 kb on + strand, within ycdU at 1095.839 kb on + strand, within ycdU at 1095.876 kb on + strand, within ycdU at 1095.888 kb on + strand, within ycdU at 1095.888 kb on + strand, within ycdU at 1095.898 kb on + strand, within ycdU at 1095.908 kb on - strand, within ycdU at 1095.943 kb on + strand, within ycdU at 1095.943 kb on + strand, within ycdU at 1096.057 kb on + strand at 1096.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan remove 1,094,690 + -0.1 1,094,690 + +1.5 1,094,710 + +1.6 1,094,710 + +0.4 1,094,715 + +0.3 1,094,728 + +0.4 1,094,733 + +0.3 1,094,815 + ymdE b1028 0.25 -0.6 1,094,846 - ymdE b1028 0.35 +2.0 1,094,880 + ymdE b1028 0.44 -0.8 1,094,881 + ymdE b1028 0.45 +0.2 1,094,888 + ymdE b1028 0.47 -1.0 1,094,888 + ymdE b1028 0.47 -1.7 1,094,888 + ymdE b1028 0.47 +0.3 1,094,888 + ymdE b1028 0.47 +1.5 1,094,913 + ymdE b1028 0.54 -0.9 1,094,914 + ymdE b1028 0.54 -0.1 1,094,921 + ymdE b1028 0.56 -0.3 1,095,010 + ymdE b1028 0.82 -1.6 1,095,010 + ymdE b1028 0.82 +0.4 1,095,010 + ymdE b1028 0.82 +0.2 1,095,020 + ymdE b1028 0.85 -0.3 1,095,020 + ymdE b1028 0.85 +0.4 1,095,020 + ymdE b1028 0.85 +3.0 1,095,022 + ymdE b1028 0.86 +0.4 1,095,022 + ymdE b1028 0.86 -0.6 1,095,022 + ymdE b1028 0.86 -0.1 1,095,022 + ymdE b1028 0.86 -0.3 1,095,032 + ymdE b1028 0.89 +1.0 1,095,402 - ycdU b1029 0.34 +2.0 1,095,408 - ycdU b1029 0.35 +2.0 1,095,442 - ycdU b1029 0.38 +2.0 1,095,499 + ycdU b1029 0.44 +0.0 1,095,566 + ycdU b1029 0.51 +2.0 1,095,566 + ycdU b1029 0.51 +0.0 1,095,567 + ycdU b1029 0.51 +2.0 1,095,581 + ycdU b1029 0.52 +1.0 1,095,597 - ycdU b1029 0.54 +2.0 1,095,597 - ycdU b1029 0.54 +2.0 1,095,612 + ycdU b1029 0.55 +0.4 1,095,650 + ycdU b1029 0.59 +0.3 1,095,650 + ycdU b1029 0.59 -1.3 1,095,650 + ycdU b1029 0.59 +0.5 1,095,652 + ycdU b1029 0.59 -2.4 1,095,699 + ycdU b1029 0.64 +0.8 1,095,701 - ycdU b1029 0.64 -0.3 1,095,730 + ycdU b1029 0.67 +0.4 1,095,730 + ycdU b1029 0.67 -0.0 1,095,730 + ycdU b1029 0.67 +0.5 1,095,740 + ycdU b1029 0.68 +2.4 1,095,782 + ycdU b1029 0.73 +0.2 1,095,839 + ycdU b1029 0.78 -0.5 1,095,876 + ycdU b1029 0.82 -0.1 1,095,888 + ycdU b1029 0.83 -0.4 1,095,888 + ycdU b1029 0.83 +1.2 1,095,898 + ycdU b1029 0.84 +1.4 1,095,908 - ycdU b1029 0.85 +2.0 1,095,943 + ycdU b1029 0.89 +0.7 1,095,943 + ycdU b1029 0.89 +0.7 1,096,057 + +1.0 1,096,067 - -0.8
Or see this region's nucleotide sequence