Strain Fitness in Escherichia coli BW25113 around b1768

Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntansA and ydjB are separated by 10 nucleotidesydjB and ydjE are separated by 92 nucleotides b1767: ansA - cytoplasmic asparaginase I (NCBI), at 1,848,884 to 1,849,900 ansA b1768: ydjB - orf, hypothetical protein (VIMSS), at 1,849,911 to 1,850,552 ydjB b1769: ydjE - predicted transporter (NCBI), at 1,850,645 to 1,852,003 ydjE Position (kb) 1849 1850 1851Strain fitness (log2 ratio) -2 -1 0 1 2at 1848.979 kb on - strandat 1848.979 kb on - strandat 1849.035 kb on - strand, within ansAat 1849.123 kb on + strand, within ansAat 1849.152 kb on + strand, within ansAat 1849.152 kb on + strand, within ansAat 1849.160 kb on - strand, within ansAat 1849.160 kb on - strand, within ansAat 1849.173 kb on - strand, within ansAat 1849.184 kb on - strand, within ansAat 1849.260 kb on - strand, within ansAat 1849.474 kb on - strand, within ansAat 1849.474 kb on - strand, within ansAat 1849.603 kb on + strand, within ansAat 1849.751 kb on + strand, within ansAat 1849.785 kb on + strand, within ansAat 1849.896 kb on + strandat 1849.896 kb on + strandat 1850.057 kb on - strand, within ydjBat 1850.262 kb on + strand, within ydjBat 1850.272 kb on - strand, within ydjBat 1850.313 kb on + strand, within ydjBat 1850.352 kb on + strand, within ydjBat 1850.353 kb on + strand, within ydjBat 1850.365 kb on + strand, within ydjBat 1850.365 kb on + strand, within ydjBat 1850.367 kb on + strand, within ydjBat 1850.372 kb on + strand, within ydjBat 1850.372 kb on + strand, within ydjBat 1850.463 kb on + strand, within ydjBat 1850.463 kb on + strand, within ydjBat 1850.463 kb on + strand, within ydjBat 1850.484 kb on + strand, within ydjBat 1850.523 kb on + strandat 1850.552 kb on + strandat 1850.552 kb on + strandat 1850.567 kb on + strandat 1850.567 kb on + strandat 1850.570 kb on + strandat 1850.570 kb on + strandat 1850.669 kb on + strandat 1850.718 kb on - strandat 1850.718 kb on - strandat 1850.720 kb on + strandat 1850.728 kb on + strandat 1850.728 kb on + strandat 1850.728 kb on - strandat 1850.738 kb on + strandat 1850.752 kb on - strandat 1850.795 kb on - strand, within ydjEat 1850.795 kb on - strand, within ydjEat 1850.795 kb on - strand, within ydjEat 1850.796 kb on + strand, within ydjEat 1850.796 kb on + strand, within ydjEat 1850.803 kb on + strand, within ydjEat 1850.804 kb on - strand, within ydjEat 1850.812 kb on - strand, within ydjEat 1850.816 kb on - strand, within ydjEat 1850.836 kb on + strand, within ydjEat 1850.851 kb on + strand, within ydjEat 1850.852 kb on + strand, within ydjEat 1850.853 kb on + strand, within ydjEat 1850.854 kb on + strand, within ydjEat 1850.907 kb on + strand, within ydjEat 1850.907 kb on + strand, within ydjEat 1850.942 kb on + strand, within ydjEat 1850.957 kb on + strand, within ydjEat 1850.978 kb on + strand, within ydjEat 1851.163 kb on - strand, within ydjEat 1851.176 kb on - strand, within ydjEat 1851.276 kb on - strand, within ydjEat 1851.280 kb on - strand, within ydjEat 1851.297 kb on - strand, within ydjEat 1851.312 kb on - strand, within ydjEat 1851.312 kb on - strand, within ydjEat 1851.348 kb on - strand, within ydjEat 1851.348 kb on - strand, within ydjEat 1851.364 kb on - strand, within ydjEat 1851.435 kb on - strand, within ydjEat 1851.457 kb on - strand, within ydjEat 1851.521 kb on - strand, within ydjEat 1851.521 kb on - strand, within ydjEat 1851.526 kb on - strand, within ydjE

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:10 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan
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1,848,979 - -0.4
1,848,979 - +0.3
1,849,035 - ansA b1767 0.15 +0.2
1,849,123 + ansA b1767 0.24 +0.5
1,849,152 + ansA b1767 0.26 -0.1
1,849,152 + ansA b1767 0.26 +0.7
1,849,160 - ansA b1767 0.27 +0.2
1,849,160 - ansA b1767 0.27 -0.6
1,849,173 - ansA b1767 0.28 +1.6
1,849,184 - ansA b1767 0.29 +0.2
1,849,260 - ansA b1767 0.37 -0.4
1,849,474 - ansA b1767 0.58 +1.0
1,849,474 - ansA b1767 0.58 -0.8
1,849,603 + ansA b1767 0.71 -0.3
1,849,751 + ansA b1767 0.85 +0.1
1,849,785 + ansA b1767 0.89 +0.5
1,849,896 + +0.4
1,849,896 + +0.2
1,850,057 - ydjB b1768 0.23 +0.3
1,850,262 + ydjB b1768 0.55 +0.1
1,850,272 - ydjB b1768 0.56 +0.5
1,850,313 + ydjB b1768 0.63 +0.2
1,850,352 + ydjB b1768 0.69 -0.0
1,850,353 + ydjB b1768 0.69 -0.0
1,850,365 + ydjB b1768 0.71 +0.3
1,850,365 + ydjB b1768 0.71 +0.2
1,850,367 + ydjB b1768 0.71 +0.0
1,850,372 + ydjB b1768 0.72 +0.7
1,850,372 + ydjB b1768 0.72 +0.3
1,850,463 + ydjB b1768 0.86 +0.2
1,850,463 + ydjB b1768 0.86 +0.9
1,850,463 + ydjB b1768 0.86 +0.0
1,850,484 + ydjB b1768 0.89 -1.3
1,850,523 + +0.2
1,850,552 + +0.2
1,850,552 + +0.4
1,850,567 + +0.6
1,850,567 + +1.1
1,850,570 + -1.4
1,850,570 + +0.2
1,850,669 + -0.1
1,850,718 - -0.2
1,850,718 - -0.4
1,850,720 + -0.5
1,850,728 + -0.0
1,850,728 + +0.6
1,850,728 - -0.1
1,850,738 + +0.6
1,850,752 - +0.5
1,850,795 - ydjE b1769 0.11 +0.2
1,850,795 - ydjE b1769 0.11 +0.1
1,850,795 - ydjE b1769 0.11 -0.9
1,850,796 + ydjE b1769 0.11 -2.0
1,850,796 + ydjE b1769 0.11 -0.8
1,850,803 + ydjE b1769 0.12 -0.5
1,850,804 - ydjE b1769 0.12 -0.3
1,850,812 - ydjE b1769 0.12 -0.7
1,850,816 - ydjE b1769 0.13 -1.6
1,850,836 + ydjE b1769 0.14 +0.0
1,850,851 + ydjE b1769 0.15 +1.4
1,850,852 + ydjE b1769 0.15 +0.7
1,850,853 + ydjE b1769 0.15 +1.7
1,850,854 + ydjE b1769 0.15 +0.4
1,850,907 + ydjE b1769 0.19 +1.7
1,850,907 + ydjE b1769 0.19 +0.5
1,850,942 + ydjE b1769 0.22 -1.8
1,850,957 + ydjE b1769 0.23 -0.8
1,850,978 + ydjE b1769 0.25 +1.7
1,851,163 - ydjE b1769 0.38 -0.9
1,851,176 - ydjE b1769 0.39 +1.7
1,851,276 - ydjE b1769 0.46 +1.7
1,851,280 - ydjE b1769 0.47 +0.1
1,851,297 - ydjE b1769 0.48 +2.3
1,851,312 - ydjE b1769 0.49 +0.7
1,851,312 - ydjE b1769 0.49 -1.1
1,851,348 - ydjE b1769 0.52 +0.7
1,851,348 - ydjE b1769 0.52 +1.1
1,851,364 - ydjE b1769 0.53 -0.7
1,851,435 - ydjE b1769 0.58 +1.1
1,851,457 - ydjE b1769 0.60 +0.1
1,851,521 - ydjE b1769 0.64 -1.1
1,851,521 - ydjE b1769 0.64 -0.4
1,851,526 - ydjE b1769 0.65 -0.4

Or see this region's nucleotide sequence