Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpC and btuD are separated by 77 nucleotides btuD and btuE overlap by 1 nucleotides btuE and btuC are separated by 62 nucleotides
b1708: nlpC - predicted lipoprotein (NCBI), at 1,790,291 to 1,790,755
nlpC
b1709: btuD - vitamin B12-transporter ATPase (NCBI), at 1,790,833 to 1,791,582
btuD
b1710: btuE - predicted glutathione peroxidase (NCBI), at 1,791,582 to 1,792,133
btuE
b1711: btuC - vtamin B12-transporter permease (NCBI), at 1,792,196 to 1,793,176
btuC
Position (kb)
1791
1792
1793 Strain fitness (log2 ratio)
-1
0
1
2 at 1791.016 kb on + strand, within btuD at 1791.494 kb on - strand, within btuD at 1791.821 kb on - strand, within btuE at 1791.821 kb on - strand, within btuE at 1791.931 kb on - strand, within btuE at 1792.288 kb on - strand at 1792.295 kb on + strand, within btuC at 1792.295 kb on + strand, within btuC at 1792.423 kb on - strand, within btuC at 1792.432 kb on + strand, within btuC at 1792.492 kb on + strand, within btuC at 1792.566 kb on + strand, within btuC at 1792.592 kb on - strand, within btuC at 1792.783 kb on + strand, within btuC at 1792.820 kb on - strand, within btuC at 1792.846 kb on + strand, within btuC at 1792.980 kb on + strand, within btuC at 1793.064 kb on + strand, within btuC at 1793.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan remove 1,791,016 + btuD b1709 0.24 +2.7 1,791,494 - btuD b1709 0.88 -1.0 1,791,821 - btuE b1710 0.43 -0.2 1,791,821 - btuE b1710 0.43 -0.9 1,791,931 - btuE b1710 0.63 -0.2 1,792,288 - +0.0 1,792,295 + btuC b1711 0.10 +0.2 1,792,295 + btuC b1711 0.10 -0.8 1,792,423 - btuC b1711 0.23 -0.0 1,792,432 + btuC b1711 0.24 -0.8 1,792,492 + btuC b1711 0.30 -0.2 1,792,566 + btuC b1711 0.38 +0.9 1,792,592 - btuC b1711 0.40 +0.0 1,792,783 + btuC b1711 0.60 -0.0 1,792,820 - btuC b1711 0.64 +0.8 1,792,846 + btuC b1711 0.66 +0.4 1,792,980 + btuC b1711 0.80 -0.1 1,793,064 + btuC b1711 0.88 +0.1 1,793,108 + +0.3
Or see this region's nucleotide sequence