Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livK and yhhK are separated by 423 nucleotides yhhK and livJ are separated by 187 nucleotides livJ and rpoH are separated by 270 nucleotides
b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583
livK
b3459: yhhK - hypothetical protein (NCBI), at 3,596,007 to 3,596,390
yhhK
b3460: livJ - leucine/isoleucine/valine transporter subunit (NCBI), at 3,596,578 to 3,597,681
livJ
b3461: rpoH - RNA polymerase sigma factor (NCBI), at 3,597,952 to 3,598,806
rpoH
Position (kb)
3596
3597
3598 Strain fitness (log2 ratio)
-1
0
1
2 at 3595.637 kb on + strand at 3595.669 kb on + strand at 3595.968 kb on + strand at 3595.975 kb on + strand at 3596.005 kb on + strand at 3596.005 kb on - strand at 3596.005 kb on - strand at 3596.007 kb on + strand at 3596.011 kb on - strand at 3596.012 kb on - strand at 3596.013 kb on - strand at 3596.043 kb on + strand at 3596.048 kb on + strand, within yhhK at 3596.048 kb on + strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.056 kb on - strand, within yhhK at 3596.061 kb on - strand, within yhhK at 3596.117 kb on + strand, within yhhK at 3596.128 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.163 kb on - strand, within yhhK at 3596.169 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.209 kb on + strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.251 kb on - strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.267 kb on + strand, within yhhK at 3596.288 kb on + strand, within yhhK at 3596.334 kb on + strand, within yhhK at 3596.420 kb on + strand at 3596.427 kb on + strand at 3596.427 kb on + strand at 3596.500 kb on + strand at 3596.588 kb on - strand at 3596.657 kb on + strand at 3596.737 kb on + strand, within livJ at 3596.906 kb on + strand, within livJ at 3596.914 kb on - strand, within livJ at 3596.914 kb on - strand, within livJ at 3596.994 kb on - strand, within livJ at 3596.994 kb on - strand, within livJ at 3597.006 kb on + strand, within livJ at 3597.133 kb on + strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.141 kb on - strand, within livJ at 3597.148 kb on + strand, within livJ at 3597.154 kb on + strand, within livJ at 3597.154 kb on + strand, within livJ at 3597.156 kb on - strand, within livJ at 3597.161 kb on + strand, within livJ at 3597.188 kb on + strand, within livJ at 3597.188 kb on + strand, within livJ at 3597.240 kb on + strand, within livJ at 3597.240 kb on + strand, within livJ at 3597.240 kb on - strand, within livJ at 3597.250 kb on + strand, within livJ at 3597.250 kb on + strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.251 kb on - strand, within livJ at 3597.276 kb on + strand, within livJ at 3597.312 kb on + strand, within livJ at 3597.312 kb on + strand, within livJ at 3597.320 kb on - strand, within livJ at 3597.377 kb on + strand, within livJ at 3597.377 kb on + strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.385 kb on - strand, within livJ at 3597.417 kb on - strand, within livJ at 3597.519 kb on - strand, within livJ at 3597.519 kb on - strand, within livJ at 3597.556 kb on + strand, within livJ at 3597.923 kb on + strand at 3597.931 kb on - strand at 3597.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan remove 3,595,637 + +2.1 3,595,669 + +1.7 3,595,968 + +0.0 3,595,975 + +0.3 3,596,005 + +0.3 3,596,005 - -0.3 3,596,005 - -0.7 3,596,007 + +0.1 3,596,011 - +0.6 3,596,012 - +0.2 3,596,013 - -0.4 3,596,043 + +1.0 3,596,048 + yhhK b3459 0.11 -0.5 3,596,048 + yhhK b3459 0.11 -0.8 3,596,056 - yhhK b3459 0.13 -0.1 3,596,056 - yhhK b3459 0.13 -0.7 3,596,061 - yhhK b3459 0.14 +0.3 3,596,117 + yhhK b3459 0.29 +0.9 3,596,128 - yhhK b3459 0.32 -0.8 3,596,163 - yhhK b3459 0.41 +0.6 3,596,163 - yhhK b3459 0.41 -0.5 3,596,169 + yhhK b3459 0.42 +0.5 3,596,209 + yhhK b3459 0.53 +0.5 3,596,209 + yhhK b3459 0.53 +0.1 3,596,251 - yhhK b3459 0.64 +0.5 3,596,251 - yhhK b3459 0.64 +0.2 3,596,267 + yhhK b3459 0.68 +0.3 3,596,267 + yhhK b3459 0.68 +0.6 3,596,288 + yhhK b3459 0.73 +0.6 3,596,334 + yhhK b3459 0.85 -0.2 3,596,420 + +0.4 3,596,427 + -0.1 3,596,427 + +0.4 3,596,500 + +0.2 3,596,588 - +0.3 3,596,657 + +2.0 3,596,737 + livJ b3460 0.14 +2.1 3,596,906 + livJ b3460 0.30 +2.3 3,596,914 - livJ b3460 0.30 +2.3 3,596,914 - livJ b3460 0.30 +1.9 3,596,994 - livJ b3460 0.38 +2.1 3,596,994 - livJ b3460 0.38 +1.8 3,597,006 + livJ b3460 0.39 +2.2 3,597,133 + livJ b3460 0.50 +2.2 3,597,141 - livJ b3460 0.51 +2.3 3,597,141 - livJ b3460 0.51 +2.0 3,597,141 - livJ b3460 0.51 +1.9 3,597,141 - livJ b3460 0.51 +2.2 3,597,148 + livJ b3460 0.52 +1.7 3,597,154 + livJ b3460 0.52 +1.8 3,597,154 + livJ b3460 0.52 +1.3 3,597,156 - livJ b3460 0.52 +2.1 3,597,161 + livJ b3460 0.53 +2.3 3,597,188 + livJ b3460 0.55 +1.6 3,597,188 + livJ b3460 0.55 +2.7 3,597,240 + livJ b3460 0.60 +1.9 3,597,240 + livJ b3460 0.60 +2.4 3,597,240 - livJ b3460 0.60 +2.0 3,597,250 + livJ b3460 0.61 +1.5 3,597,250 + livJ b3460 0.61 +1.9 3,597,251 - livJ b3460 0.61 +2.1 3,597,251 - livJ b3460 0.61 +2.0 3,597,251 - livJ b3460 0.61 +2.3 3,597,276 + livJ b3460 0.63 +2.1 3,597,312 + livJ b3460 0.66 +2.0 3,597,312 + livJ b3460 0.66 +2.1 3,597,320 - livJ b3460 0.67 +2.1 3,597,377 + livJ b3460 0.72 +2.5 3,597,377 + livJ b3460 0.72 +2.2 3,597,385 - livJ b3460 0.73 +1.8 3,597,385 - livJ b3460 0.73 +2.4 3,597,385 - livJ b3460 0.73 +2.0 3,597,385 - livJ b3460 0.73 +1.8 3,597,417 - livJ b3460 0.76 +2.1 3,597,519 - livJ b3460 0.85 +2.1 3,597,519 - livJ b3460 0.85 +2.2 3,597,556 + livJ b3460 0.89 +1.8 3,597,923 + +0.2 3,597,931 - -0.1 3,597,931 - -1.6
Or see this region's nucleotide sequence