Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livM and livH overlap by 4 nucleotides livH and livK are separated by 47 nucleotides
b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503
livM
b3457: livH - leucine/isoleucine/valine transporter subunit (NCBI), at 3,593,500 to 3,594,426
livH
b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583
livK
Position (kb)
3593
3594
3595 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3592.590 kb on - strand, within livM at 3592.640 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.834 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.103 kb on - strand, within livM at 3593.103 kb on - strand, within livM at 3593.139 kb on + strand, within livM at 3593.210 kb on + strand, within livM at 3593.210 kb on + strand, within livM at 3593.244 kb on + strand, within livM at 3593.339 kb on + strand, within livM at 3593.375 kb on - strand, within livM at 3593.383 kb on - strand at 3593.383 kb on - strand at 3593.401 kb on + strand at 3593.409 kb on - strand at 3593.424 kb on - strand at 3593.438 kb on - strand at 3593.438 kb on - strand at 3593.524 kb on + strand at 3593.620 kb on - strand, within livH at 3593.620 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.660 kb on + strand, within livH at 3593.660 kb on + strand, within livH at 3593.749 kb on + strand, within livH at 3593.825 kb on + strand, within livH at 3593.833 kb on - strand, within livH at 3593.869 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.966 kb on - strand, within livH at 3594.055 kb on - strand, within livH at 3594.098 kb on - strand, within livH at 3594.150 kb on + strand, within livH at 3594.150 kb on + strand, within livH at 3594.220 kb on + strand, within livH at 3594.232 kb on - strand, within livH at 3594.232 kb on - strand, within livH at 3594.236 kb on + strand, within livH at 3594.236 kb on + strand, within livH at 3594.346 kb on - strand at 3594.350 kb on - strand at 3594.350 kb on - strand at 3594.404 kb on - strand at 3594.404 kb on - strand at 3594.417 kb on + strand at 3594.469 kb on + strand at 3594.485 kb on - strand at 3594.518 kb on - strand at 3594.593 kb on + strand, within livK at 3594.638 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.639 kb on + strand, within livK at 3594.743 kb on - strand, within livK at 3594.821 kb on + strand, within livK at 3594.876 kb on + strand, within livK at 3594.876 kb on + strand, within livK at 3594.908 kb on + strand, within livK at 3594.954 kb on + strand, within livK at 3595.035 kb on + strand, within livK at 3595.043 kb on - strand, within livK at 3595.053 kb on - strand, within livK at 3595.097 kb on + strand, within livK at 3595.109 kb on - strand, within livK at 3595.142 kb on + strand, within livK at 3595.199 kb on + strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.207 kb on - strand, within livK at 3595.264 kb on + strand, within livK at 3595.276 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.343 kb on - strand, within livK at 3595.354 kb on - strand, within livK at 3595.365 kb on + strand, within livK
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan remove 3,592,590 - livM b3456 0.28 +3.1 3,592,640 + livM b3456 0.32 +2.9 3,592,763 + livM b3456 0.42 +3.0 3,592,763 + livM b3456 0.42 +2.6 3,592,768 + livM b3456 0.42 +2.7 3,592,768 + livM b3456 0.42 +2.7 3,592,822 + livM b3456 0.47 +2.9 3,592,822 + livM b3456 0.47 +2.8 3,592,834 + livM b3456 0.48 +2.8 3,593,081 + livM b3456 0.67 +2.9 3,593,081 + livM b3456 0.67 +2.9 3,593,103 - livM b3456 0.69 +2.9 3,593,103 - livM b3456 0.69 +2.6 3,593,139 + livM b3456 0.71 +2.9 3,593,210 + livM b3456 0.77 +2.8 3,593,210 + livM b3456 0.77 +2.9 3,593,244 + livM b3456 0.80 +2.5 3,593,339 + livM b3456 0.87 +2.6 3,593,375 - livM b3456 0.90 +2.8 3,593,383 - +2.4 3,593,383 - +3.0 3,593,401 + +2.8 3,593,409 - +2.4 3,593,424 - +2.0 3,593,438 - +2.4 3,593,438 - +2.6 3,593,524 + +2.6 3,593,620 - livH b3457 0.13 +2.8 3,593,620 - livH b3457 0.13 +2.6 3,593,655 - livH b3457 0.17 +2.1 3,593,655 - livH b3457 0.17 +2.3 3,593,655 - livH b3457 0.17 +2.2 3,593,655 - livH b3457 0.17 +3.2 3,593,660 + livH b3457 0.17 +2.7 3,593,660 + livH b3457 0.17 +2.7 3,593,749 + livH b3457 0.27 +2.7 3,593,825 + livH b3457 0.35 +1.8 3,593,833 - livH b3457 0.36 +2.6 3,593,869 + livH b3457 0.40 +2.8 3,593,942 + livH b3457 0.48 +2.7 3,593,942 + livH b3457 0.48 +2.8 3,593,966 - livH b3457 0.50 +2.7 3,594,055 - livH b3457 0.60 +2.5 3,594,098 - livH b3457 0.65 +2.8 3,594,150 + livH b3457 0.70 +2.8 3,594,150 + livH b3457 0.70 +3.0 3,594,220 + livH b3457 0.78 +2.7 3,594,232 - livH b3457 0.79 +2.3 3,594,232 - livH b3457 0.79 +3.1 3,594,236 + livH b3457 0.79 +2.7 3,594,236 + livH b3457 0.79 +3.0 3,594,346 - +2.6 3,594,350 - +2.4 3,594,350 - +2.3 3,594,404 - +2.3 3,594,404 - +3.1 3,594,417 + +2.8 3,594,469 + +2.3 3,594,485 - -0.5 3,594,518 - +2.3 3,594,593 + livK b3458 0.11 +1.7 3,594,638 + livK b3458 0.15 +1.8 3,594,639 + livK b3458 0.15 +1.8 3,594,639 + livK b3458 0.15 +1.8 3,594,639 + livK b3458 0.15 +2.0 3,594,639 + livK b3458 0.15 +2.2 3,594,743 - livK b3458 0.24 -0.7 3,594,821 + livK b3458 0.31 +1.5 3,594,876 + livK b3458 0.36 +2.4 3,594,876 + livK b3458 0.36 +2.2 3,594,908 + livK b3458 0.39 +2.2 3,594,954 + livK b3458 0.43 +1.9 3,595,035 + livK b3458 0.51 +2.0 3,595,043 - livK b3458 0.51 -0.2 3,595,053 - livK b3458 0.52 -0.0 3,595,097 + livK b3458 0.56 +2.2 3,595,109 - livK b3458 0.57 +0.1 3,595,142 + livK b3458 0.60 +1.8 3,595,199 + livK b3458 0.65 +2.8 3,595,207 - livK b3458 0.66 +0.8 3,595,207 - livK b3458 0.66 +0.4 3,595,207 - livK b3458 0.66 +0.2 3,595,264 + livK b3458 0.71 +2.6 3,595,276 - livK b3458 0.72 +1.2 3,595,343 - livK b3458 0.78 +1.4 3,595,343 - livK b3458 0.78 +2.1 3,595,354 - livK b3458 0.79 +1.6 3,595,365 + livK b3458 0.80 +2.8
Or see this region's nucleotide sequence