Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livF and livG are separated by 1 nucleotides livG and livM overlap by 4 nucleotides
b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460
livF
b3455: livG - leucine/isoleucine/valine transporter subunit (NCBI), at 3,591,462 to 3,592,229
livG
b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503
livM
Position (kb)
3591
3592
3593 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3590.655 kb on + strand at 3590.659 kb on - strand at 3590.659 kb on - strand at 3590.703 kb on - strand at 3590.774 kb on - strand at 3590.810 kb on - strand at 3590.898 kb on - strand, within livF at 3590.898 kb on - strand, within livF at 3590.903 kb on - strand, within livF at 3590.966 kb on - strand, within livF at 3590.966 kb on - strand, within livF at 3590.988 kb on + strand, within livF at 3591.059 kb on - strand, within livF at 3591.061 kb on + strand, within livF at 3591.090 kb on - strand, within livF at 3591.125 kb on - strand, within livF at 3591.164 kb on + strand, within livF at 3591.164 kb on + strand, within livF at 3591.172 kb on - strand, within livF at 3591.197 kb on + strand, within livF at 3591.252 kb on + strand, within livF at 3591.252 kb on + strand, within livF at 3591.381 kb on - strand, within livF at 3591.381 kb on - strand, within livF at 3591.419 kb on - strand at 3591.479 kb on - strand at 3591.479 kb on - strand at 3591.547 kb on - strand, within livG at 3591.584 kb on + strand, within livG at 3591.592 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.765 kb on + strand, within livG at 3591.768 kb on - strand, within livG at 3591.834 kb on + strand, within livG at 3591.834 kb on + strand, within livG at 3591.881 kb on + strand, within livG at 3591.907 kb on + strand, within livG at 3591.937 kb on - strand, within livG at 3591.943 kb on - strand, within livG at 3591.962 kb on - strand, within livG at 3591.974 kb on - strand, within livG at 3592.053 kb on + strand, within livG at 3592.148 kb on - strand, within livG at 3592.229 kb on + strand at 3592.321 kb on - strand at 3592.436 kb on + strand, within livM at 3592.436 kb on + strand, within livM at 3592.590 kb on - strand, within livM at 3592.640 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.834 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.103 kb on - strand, within livM at 3593.103 kb on - strand, within livM at 3593.139 kb on + strand, within livM at 3593.210 kb on + strand, within livM at 3593.210 kb on + strand, within livM
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:100 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan remove 3,590,655 + +0.5 3,590,659 - +0.1 3,590,659 - -0.2 3,590,703 - +0.8 3,590,774 - +2.5 3,590,810 - +2.5 3,590,898 - livF b3454 0.21 +2.2 3,590,898 - livF b3454 0.21 +2.3 3,590,903 - livF b3454 0.22 +2.7 3,590,966 - livF b3454 0.31 +2.4 3,590,966 - livF b3454 0.31 +3.1 3,590,988 + livF b3454 0.34 +2.9 3,591,059 - livF b3454 0.44 +2.3 3,591,061 + livF b3454 0.44 +2.5 3,591,090 - livF b3454 0.48 +2.5 3,591,125 - livF b3454 0.53 +3.1 3,591,164 + livF b3454 0.58 +2.6 3,591,164 + livF b3454 0.58 +2.2 3,591,172 - livF b3454 0.60 +2.7 3,591,197 + livF b3454 0.63 +2.6 3,591,252 + livF b3454 0.71 +2.7 3,591,252 + livF b3454 0.71 +2.8 3,591,381 - livF b3454 0.89 +2.6 3,591,381 - livF b3454 0.89 +2.3 3,591,419 - +2.4 3,591,479 - +1.6 3,591,479 - +1.5 3,591,547 - livG b3455 0.11 +2.7 3,591,584 + livG b3455 0.16 +2.5 3,591,592 - livG b3455 0.17 +2.3 3,591,748 - livG b3455 0.37 +2.5 3,591,748 - livG b3455 0.37 +2.7 3,591,748 - livG b3455 0.37 +3.2 3,591,765 + livG b3455 0.39 +2.6 3,591,768 - livG b3455 0.40 +2.5 3,591,834 + livG b3455 0.48 +2.9 3,591,834 + livG b3455 0.48 +2.5 3,591,881 + livG b3455 0.55 +3.0 3,591,907 + livG b3455 0.58 +2.7 3,591,937 - livG b3455 0.62 +3.0 3,591,943 - livG b3455 0.63 +2.5 3,591,962 - livG b3455 0.65 +2.3 3,591,974 - livG b3455 0.67 +2.3 3,592,053 + livG b3455 0.77 +2.7 3,592,148 - livG b3455 0.89 +2.6 3,592,229 + +2.0 3,592,321 - +2.6 3,592,436 + livM b3456 0.16 +2.8 3,592,436 + livM b3456 0.16 +2.5 3,592,590 - livM b3456 0.28 +3.1 3,592,640 + livM b3456 0.32 +2.9 3,592,763 + livM b3456 0.42 +3.0 3,592,763 + livM b3456 0.42 +2.6 3,592,768 + livM b3456 0.42 +2.7 3,592,768 + livM b3456 0.42 +2.7 3,592,822 + livM b3456 0.47 +2.9 3,592,822 + livM b3456 0.47 +2.8 3,592,834 + livM b3456 0.48 +2.8 3,593,081 + livM b3456 0.67 +2.9 3,593,081 + livM b3456 0.67 +2.9 3,593,103 - livM b3456 0.69 +2.9 3,593,103 - livM b3456 0.69 +2.6 3,593,139 + livM b3456 0.71 +2.9 3,593,210 + livM b3456 0.77 +2.8 3,593,210 + livM b3456 0.77 +2.9
Or see this region's nucleotide sequence