Strain Fitness in Escherichia coli BW25113 around b3013

Experiment: Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqhE and yqhG are separated by 199 nucleotidesyqhG and yqhH are separated by 50 nucleotidesyqhH and ygiQ are separated by 42 nucleotides b3012: yqhE - orf, hypothetical protein (VIMSS), at 3,154,645 to 3,155,472 yqhE b3013: yqhG - hypothetical protein (NCBI), at 3,155,672 to 3,156,598 yqhG b3014: yqhH - predicted outer membrane lipoprotein (NCBI), at 3,156,649 to 3,156,906 yqhH b4469: ygiQ - hypothetical protein (NCBI), at 3,156,949 to 3,159,168 ygiQ Position (kb) 3155 3156 3157Strain fitness (log2 ratio) -3 -2 -1 0 1at 3154.694 kb on + strandat 3154.702 kb on - strandat 3154.821 kb on - strand, within yqhEat 3154.821 kb on - strand, within yqhEat 3154.906 kb on + strand, within yqhEat 3154.909 kb on + strand, within yqhEat 3154.919 kb on + strand, within yqhEat 3154.963 kb on - strand, within yqhEat 3154.976 kb on - strand, within yqhEat 3154.976 kb on - strand, within yqhEat 3155.101 kb on + strand, within yqhEat 3155.121 kb on - strand, within yqhEat 3155.121 kb on - strand, within yqhEat 3155.160 kb on - strand, within yqhEat 3155.163 kb on + strand, within yqhEat 3155.163 kb on + strand, within yqhEat 3155.163 kb on + strand, within yqhEat 3155.271 kb on - strand, within yqhEat 3155.318 kb on + strand, within yqhEat 3155.318 kb on + strand, within yqhEat 3155.382 kb on - strand, within yqhEat 3155.382 kb on - strand, within yqhEat 3155.382 kb on - strandat 3155.382 kb on - strand, within yqhEat 3155.431 kb on + strandat 3155.449 kb on + strandat 3155.458 kb on - strandat 3155.493 kb on + strandat 3155.582 kb on + strandat 3155.601 kb on - strandat 3155.605 kb on + strandat 3155.627 kb on + strandat 3155.627 kb on + strandat 3155.630 kb on + strandat 3155.632 kb on + strandat 3155.632 kb on + strandat 3155.648 kb on + strandat 3155.726 kb on - strandat 3155.732 kb on + strandat 3155.734 kb on + strandat 3155.734 kb on + strandat 3155.738 kb on - strandat 3155.740 kb on - strandat 3155.740 kb on - strandat 3155.742 kb on - strandat 3155.750 kb on - strandat 3155.827 kb on + strand, within yqhGat 3155.827 kb on + strand, within yqhGat 3155.841 kb on + strand, within yqhGat 3155.844 kb on + strand, within yqhGat 3155.844 kb on + strand, within yqhGat 3155.846 kb on + strand, within yqhGat 3155.857 kb on + strand, within yqhGat 3155.861 kb on - strand, within yqhGat 3155.889 kb on + strand, within yqhGat 3155.955 kb on + strand, within yqhGat 3155.955 kb on + strand, within yqhGat 3155.981 kb on + strand, within yqhGat 3156.131 kb on - strand, within yqhGat 3156.363 kb on + strand, within yqhGat 3156.679 kb on - strand, within yqhHat 3156.691 kb on + strand, within yqhHat 3156.783 kb on + strand, within yqhHat 3156.783 kb on + strand, within yqhHat 3156.791 kb on - strand, within yqhHat 3156.791 kb on - strand, within yqhHat 3156.856 kb on + strand, within yqhHat 3156.859 kb on - strand, within yqhHat 3156.859 kb on - strand, within yqhHat 3157.016 kb on - strandat 3157.048 kb on - strandat 3157.087 kb on + strandat 3157.335 kb on + strand, within ygiQat 3157.335 kb on + strand, within ygiQat 3157.383 kb on - strand, within ygiQat 3157.421 kb on + strand, within ygiQat 3157.456 kb on - strand, within ygiQat 3157.486 kb on - strand, within ygiQat 3157.486 kb on - strand, within ygiQat 3157.527 kb on + strand, within ygiQat 3157.536 kb on + strand, within ygiQat 3157.536 kb on + strand, within ygiQat 3157.536 kb on + strand, within ygiQat 3157.541 kb on + strand, within ygiQat 3157.565 kb on + strand, within ygiQat 3157.565 kb on + strand, within ygiQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus str. Tiberius 1:10 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
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3,154,694 + -0.2
3,154,702 - +0.0
3,154,821 - yqhE b3012 0.21 -0.2
3,154,821 - yqhE b3012 0.21 +0.4
3,154,906 + yqhE b3012 0.32 +0.8
3,154,909 + yqhE b3012 0.32 -0.5
3,154,919 + yqhE b3012 0.33 -0.7
3,154,963 - yqhE b3012 0.38 -0.2
3,154,976 - yqhE b3012 0.40 -0.2
3,154,976 - yqhE b3012 0.40 -0.2
3,155,101 + yqhE b3012 0.55 -0.4
3,155,121 - yqhE b3012 0.57 +0.2
3,155,121 - yqhE b3012 0.57 -0.5
3,155,160 - yqhE b3012 0.62 -0.3
3,155,163 + yqhE b3012 0.63 +0.4
3,155,163 + yqhE b3012 0.63 +0.3
3,155,163 + yqhE b3012 0.63 -0.1
3,155,271 - yqhE b3012 0.76 +0.6
3,155,318 + yqhE b3012 0.81 +0.7
3,155,318 + yqhE b3012 0.81 -1.9
3,155,382 - yqhE b3012 0.89 +0.8
3,155,382 - yqhE b3012 0.89 +0.4
3,155,382 - +1.2
3,155,382 - yqhE b3012 0.89 +0.2
3,155,431 + +0.5
3,155,449 + -0.1
3,155,458 - +0.8
3,155,493 + +0.4
3,155,582 + +0.2
3,155,601 - +0.6
3,155,605 + -1.6
3,155,627 + -0.8
3,155,627 + -1.0
3,155,630 + +0.4
3,155,632 + -1.9
3,155,632 + -1.7
3,155,648 + +0.8
3,155,726 - +0.2
3,155,732 + +0.5
3,155,734 + -0.3
3,155,734 + -0.8
3,155,738 - +0.3
3,155,740 - +0.3
3,155,740 - -1.3
3,155,742 - -1.9
3,155,750 - +0.3
3,155,827 + yqhG b3013 0.17 -0.5
3,155,827 + yqhG b3013 0.17 +0.0
3,155,841 + yqhG b3013 0.18 +0.1
3,155,844 + yqhG b3013 0.19 -0.6
3,155,844 + yqhG b3013 0.19 +0.3
3,155,846 + yqhG b3013 0.19 +0.5
3,155,857 + yqhG b3013 0.20 +0.6
3,155,861 - yqhG b3013 0.20 +0.4
3,155,889 + yqhG b3013 0.23 -2.0
3,155,955 + yqhG b3013 0.31 -0.2
3,155,955 + yqhG b3013 0.31 +0.9
3,155,981 + yqhG b3013 0.33 +0.3
3,156,131 - yqhG b3013 0.50 -0.1
3,156,363 + yqhG b3013 0.75 -1.2
3,156,679 - yqhH b3014 0.12 +1.0
3,156,691 + yqhH b3014 0.16 +0.1
3,156,783 + yqhH b3014 0.52 +0.2
3,156,783 + yqhH b3014 0.52 -0.7
3,156,791 - yqhH b3014 0.55 -1.3
3,156,791 - yqhH b3014 0.55 +0.1
3,156,856 + yqhH b3014 0.80 +0.3
3,156,859 - yqhH b3014 0.81 +0.3
3,156,859 - yqhH b3014 0.81 -0.1
3,157,016 - +0.2
3,157,048 - -1.6
3,157,087 + +0.8
3,157,335 + ygiQ b4469 0.17 -0.9
3,157,335 + ygiQ b4469 0.17 +0.7
3,157,383 - ygiQ b4469 0.20 +0.2
3,157,421 + ygiQ b4469 0.21 -0.5
3,157,456 - ygiQ b4469 0.23 +1.0
3,157,486 - ygiQ b4469 0.24 -0.6
3,157,486 - ygiQ b4469 0.24 -0.6
3,157,527 + ygiQ b4469 0.26 -3.1
3,157,536 + ygiQ b4469 0.26 -0.1
3,157,536 + ygiQ b4469 0.26 -1.0
3,157,536 + ygiQ b4469 0.26 +0.0
3,157,541 + ygiQ b4469 0.27 +0.0
3,157,565 + ygiQ b4469 0.28 +0.8
3,157,565 + ygiQ b4469 0.28 -0.1

Or see this region's nucleotide sequence