Experiment: Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hns and tdk are separated by 604 nucleotides tdk and insZ are separated by 281 nucleotides
b1237: hns - global DNA-binding transcriptional dual regulator H-NS (NCBI), at 1,291,732 to 1,292,145
hns
b1238: tdk - thymidine kinase (NCBI), at 1,292,750 to 1,293,367
tdk
b4573: insZ - no description, at 1,293,649 to 1,294,215
insZ
Position (kb)
1292
1293
1294 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1291.886 kb on - strand, within hns at 1292.167 kb on - strand at 1292.218 kb on + strand at 1292.319 kb on - strand at 1292.353 kb on - strand at 1292.383 kb on + strand at 1292.394 kb on + strand at 1292.401 kb on + strand at 1292.409 kb on - strand at 1292.417 kb on - strand at 1292.419 kb on - strand at 1292.469 kb on - strand at 1292.469 kb on - strand at 1292.481 kb on + strand at 1292.497 kb on - strand at 1292.504 kb on + strand at 1292.504 kb on + strand at 1292.508 kb on + strand at 1292.512 kb on - strand at 1292.512 kb on - strand at 1292.519 kb on + strand at 1292.522 kb on - strand at 1292.522 kb on - strand at 1292.527 kb on - strand at 1292.527 kb on - strand at 1292.532 kb on - strand at 1292.537 kb on - strand at 1292.549 kb on - strand at 1292.549 kb on - strand at 1292.552 kb on + strand at 1292.552 kb on - strand at 1292.562 kb on + strand at 1292.572 kb on + strand at 1292.592 kb on - strand at 1292.602 kb on + strand at 1292.604 kb on + strand at 1292.604 kb on + strand at 1292.623 kb on + strand at 1292.623 kb on + strand at 1292.627 kb on + strand at 1292.635 kb on - strand at 1292.644 kb on + strand at 1292.644 kb on + strand at 1292.645 kb on - strand at 1292.645 kb on - strand at 1292.645 kb on - strand at 1292.647 kb on + strand at 1292.652 kb on + strand at 1292.661 kb on + strand at 1292.661 kb on + strand at 1292.665 kb on - strand at 1292.665 kb on - strand at 1292.672 kb on - strand at 1292.682 kb on - strand at 1292.682 kb on - strand at 1292.700 kb on + strand at 1292.735 kb on - strand at 1292.738 kb on + strand at 1292.738 kb on + strand at 1292.739 kb on + strand at 1292.739 kb on + strand at 1292.749 kb on - strand at 1292.794 kb on + strand at 1292.794 kb on + strand at 1292.797 kb on - strand at 1292.798 kb on + strand at 1292.862 kb on - strand, within tdk at 1292.896 kb on - strand, within tdk at 1292.898 kb on + strand, within tdk at 1292.909 kb on + strand, within tdk at 1292.944 kb on - strand, within tdk at 1292.957 kb on + strand, within tdk at 1293.067 kb on + strand, within tdk at 1293.067 kb on + strand, within tdk at 1293.146 kb on - strand, within tdk at 1293.192 kb on + strand, within tdk at 1293.200 kb on - strand, within tdk at 1293.209 kb on + strand, within tdk at 1293.211 kb on - strand, within tdk at 1293.214 kb on - strand, within tdk at 1293.217 kb on - strand, within tdk at 1293.217 kb on - strand, within tdk at 1293.224 kb on - strand, within tdk at 1293.238 kb on + strand, within tdk at 1293.245 kb on - strand, within tdk at 1293.245 kb on - strand, within tdk at 1293.270 kb on + strand, within tdk at 1293.285 kb on + strand, within tdk at 1293.324 kb on - strand at 1293.384 kb on + strand at 1293.384 kb on + strand at 1293.397 kb on + strand at 1293.471 kb on + strand at 1293.473 kb on + strand at 1293.473 kb on + strand at 1293.479 kb on - strand at 1293.570 kb on + strand at 1293.576 kb on + strand at 1293.576 kb on + strand at 1293.584 kb on - strand at 1293.637 kb on + strand at 1293.637 kb on + strand at 1293.645 kb on - strand at 1293.652 kb on + strand at 1293.750 kb on + strand, within insZ at 1293.800 kb on - strand, within insZ at 1293.965 kb on + strand, within insZ at 1294.028 kb on - strand, within insZ at 1294.043 kb on - strand, within insZ at 1294.043 kb on - strand, within insZ at 1294.144 kb on - strand, within insZ at 1294.175 kb on - strand at 1294.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan remove 1,291,886 - hns b1237 0.37 -0.1 1,292,167 - -2.5 1,292,218 + +0.2 1,292,319 - -0.3 1,292,353 - +0.2 1,292,383 + -0.1 1,292,394 + -0.7 1,292,401 + -1.9 1,292,409 - -0.1 1,292,417 - +0.1 1,292,419 - +0.7 1,292,469 - +0.8 1,292,469 - +0.8 1,292,481 + -0.1 1,292,497 - +0.7 1,292,504 + +0.7 1,292,504 + +2.4 1,292,508 + -2.2 1,292,512 - +1.2 1,292,512 - +0.6 1,292,519 + -0.1 1,292,522 - +0.2 1,292,522 - +0.2 1,292,527 - -0.1 1,292,527 - -0.2 1,292,532 - +0.5 1,292,537 - -1.1 1,292,549 - -0.7 1,292,549 - +0.2 1,292,552 + -0.1 1,292,552 - -0.2 1,292,562 + +0.3 1,292,572 + +0.7 1,292,592 - -1.1 1,292,602 + -1.4 1,292,604 + +2.2 1,292,604 + +0.4 1,292,623 + -0.1 1,292,623 + +0.4 1,292,627 + +0.6 1,292,635 - +1.0 1,292,644 + -1.1 1,292,644 + -0.8 1,292,645 - +3.0 1,292,645 - +1.4 1,292,645 - +0.4 1,292,647 + +1.0 1,292,652 + +0.3 1,292,661 + -0.4 1,292,661 + +0.3 1,292,665 - +0.1 1,292,665 - -0.6 1,292,672 - -0.1 1,292,682 - +1.4 1,292,682 - +1.4 1,292,700 + -1.4 1,292,735 - +1.4 1,292,738 + -2.0 1,292,738 + +1.0 1,292,739 + +0.1 1,292,739 + -1.8 1,292,749 - +0.4 1,292,794 + +0.3 1,292,794 + -0.4 1,292,797 - +1.0 1,292,798 + +0.1 1,292,862 - tdk b1238 0.18 -0.1 1,292,896 - tdk b1238 0.24 +1.4 1,292,898 + tdk b1238 0.24 +1.1 1,292,909 + tdk b1238 0.26 -0.6 1,292,944 - tdk b1238 0.31 -1.8 1,292,957 + tdk b1238 0.33 +1.8 1,293,067 + tdk b1238 0.51 +0.1 1,293,067 + tdk b1238 0.51 -0.6 1,293,146 - tdk b1238 0.64 -2.1 1,293,192 + tdk b1238 0.72 +0.2 1,293,200 - tdk b1238 0.73 +0.4 1,293,209 + tdk b1238 0.74 -0.3 1,293,211 - tdk b1238 0.75 -0.6 1,293,214 - tdk b1238 0.75 +0.2 1,293,217 - tdk b1238 0.76 -0.1 1,293,217 - tdk b1238 0.76 -0.6 1,293,224 - tdk b1238 0.77 -0.1 1,293,238 + tdk b1238 0.79 +0.2 1,293,245 - tdk b1238 0.80 -0.6 1,293,245 - tdk b1238 0.80 -0.3 1,293,270 + tdk b1238 0.84 -0.1 1,293,285 + tdk b1238 0.87 +0.4 1,293,324 - +1.4 1,293,384 + +0.6 1,293,384 + -0.3 1,293,397 + +1.0 1,293,471 + +0.4 1,293,473 + -0.3 1,293,473 + -0.1 1,293,479 - -0.1 1,293,570 + +0.9 1,293,576 + +0.2 1,293,576 + +0.0 1,293,584 - -0.3 1,293,637 + -0.3 1,293,637 + +0.2 1,293,645 - +0.5 1,293,652 + +0.5 1,293,750 + insZ b4573 0.18 +0.0 1,293,800 - insZ b4573 0.27 -0.0 1,293,965 + insZ b4573 0.56 +0.3 1,294,028 - insZ b4573 0.67 +0.4 1,294,043 - insZ b4573 0.69 +0.1 1,294,043 - insZ b4573 0.69 +0.0 1,294,144 - insZ b4573 0.87 -0.9 1,294,175 - +0.4 1,294,207 + +0.0
Or see this region's nucleotide sequence