Strain Fitness in Escherichia coli BW25113 around b2947

Experiment: 18 hr rep1_HD100_10fold_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntendA and yggJ are separated by 79 nucleotidesyggJ and gshB are separated by 12 nucleotidesgshB and yqgE are separated by 108 nucleotidesyqgE and yqgF overlap by 1 nucleotides b2945: endA - DNA-specific endonuclease I (NCBI), at 3,088,369 to 3,089,076 endA b2946: yggJ - orf, hypothetical protein (VIMSS), at 3,089,156 to 3,089,887 yggJ b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850 gshB b2948: yqgE - orf, hypothetical protein (VIMSS), at 3,090,959 to 3,091,522 yqgE b2949: yqgF - Holliday junction resolvase-like protein (NCBI), at 3,091,522 to 3,091,938 yqgF Position (kb) 3089 3090 3091Strain fitness (log2 ratio) -2 -1 0 1 2at 3088.962 kb on + strand, within endAat 3088.962 kb on + strand, within endAat 3088.962 kb on + strand, within endAat 3088.970 kb on - strand, within endAat 3088.970 kb on - strand, within endAat 3089.020 kb on - strandat 3089.038 kb on - strandat 3089.038 kb on - strandat 3089.038 kb on - strandat 3089.191 kb on + strandat 3089.281 kb on - strand, within yggJat 3089.295 kb on - strand, within yggJat 3089.411 kb on + strand, within yggJat 3089.476 kb on - strand, within yggJat 3089.476 kb on - strand, within yggJat 3089.586 kb on - strand, within yggJat 3089.586 kb on - strand, within yggJat 3089.634 kb on + strand, within yggJat 3089.634 kb on + strand, within yggJat 3089.642 kb on - strand, within yggJat 3089.642 kb on - strand, within yggJat 3089.895 kb on + strandat 3089.924 kb on - strandat 3090.004 kb on + strand, within gshBat 3090.085 kb on - strand, within gshBat 3090.085 kb on - strand, within gshBat 3090.092 kb on - strand, within gshBat 3090.222 kb on + strand, within gshBat 3090.458 kb on - strand, within gshBat 3090.479 kb on - strand, within gshBat 3090.479 kb on - strand, within gshBat 3090.486 kb on - strand, within gshBat 3090.562 kb on - strand, within gshBat 3090.562 kb on - strand, within gshBat 3090.576 kb on + strand, within gshBat 3090.811 kb on + strandat 3090.847 kb on - strandat 3090.847 kb on - strandat 3090.883 kb on + strandat 3090.883 kb on + strandat 3090.893 kb on - strandat 3090.893 kb on - strandat 3091.078 kb on - strand, within yqgEat 3091.078 kb on - strand, within yqgEat 3091.140 kb on + strand, within yqgEat 3091.140 kb on + strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.271 kb on - strand, within yqgEat 3091.328 kb on + strand, within yqgEat 3091.534 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 18 hr rep1_HD100_10fold_dilution
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3,088,962 + endA b2945 0.84 -0.0
3,088,962 + endA b2945 0.84 +0.2
3,088,962 + endA b2945 0.84 +0.5
3,088,970 - endA b2945 0.85 -0.5
3,088,970 - endA b2945 0.85 -1.1
3,089,020 - -0.1
3,089,038 - -0.8
3,089,038 - -1.1
3,089,038 - +0.4
3,089,191 + -0.3
3,089,281 - yggJ b2946 0.17 -0.1
3,089,295 - yggJ b2946 0.19 +0.3
3,089,411 + yggJ b2946 0.35 -1.1
3,089,476 - yggJ b2946 0.44 +0.1
3,089,476 - yggJ b2946 0.44 -0.2
3,089,586 - yggJ b2946 0.59 +0.4
3,089,586 - yggJ b2946 0.59 +0.5
3,089,634 + yggJ b2946 0.65 +0.4
3,089,634 + yggJ b2946 0.65 +0.3
3,089,642 - yggJ b2946 0.66 -0.0
3,089,642 - yggJ b2946 0.66 -1.1
3,089,895 + -0.3
3,089,924 - -0.0
3,090,004 + gshB b2947 0.11 +0.4
3,090,085 - gshB b2947 0.19 +0.3
3,090,085 - gshB b2947 0.19 +0.4
3,090,092 - gshB b2947 0.20 -0.6
3,090,222 + gshB b2947 0.34 +0.5
3,090,458 - gshB b2947 0.59 -0.1
3,090,479 - gshB b2947 0.61 -0.0
3,090,479 - gshB b2947 0.61 +0.3
3,090,486 - gshB b2947 0.62 +1.3
3,090,562 - gshB b2947 0.70 -0.5
3,090,562 - gshB b2947 0.70 -0.5
3,090,576 + gshB b2947 0.71 -1.1
3,090,811 + -0.5
3,090,847 - -0.3
3,090,847 - -0.1
3,090,883 + -0.2
3,090,883 + +0.8
3,090,893 - +0.7
3,090,893 - +2.1
3,091,078 - yqgE b2948 0.21 +0.3
3,091,078 - yqgE b2948 0.21 -0.1
3,091,140 + yqgE b2948 0.32 +0.3
3,091,140 + yqgE b2948 0.32 +0.8
3,091,271 - yqgE b2948 0.55 -1.1
3,091,271 - yqgE b2948 0.55 -0.3
3,091,328 + yqgE b2948 0.65 -0.2
3,091,534 + -1.8

Or see this region's nucleotide sequence