Strain Fitness in Escherichia coli BW25113 around b3959

Experiment: 18 hr rep1_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntargC and argB are separated by 7 nucleotidesargB and argH are separated by 60 nucleotides b3958: argC - N-acetyl-gamma-glutamyl-phosphate reductase (NCBI), at 4,153,024 to 4,154,028 argC b3959: argB - acetylglutamate kinase (RefSeq), at 4,154,036 to 4,154,812 argB b3960: argH - argininosuccinate lyase (NCBI), at 4,154,873 to 4,156,246 argH Position (kb) 4154 4155Strain fitness (log2 ratio) -2 -1 0 1 2at 4153.072 kb on - strandat 4153.072 kb on - strandat 4153.082 kb on + strandat 4153.082 kb on + strandat 4153.087 kb on - strandat 4153.101 kb on + strandat 4153.137 kb on - strand, within argCat 4153.184 kb on - strand, within argCat 4153.208 kb on - strand, within argCat 4153.208 kb on - strand, within argCat 4153.240 kb on - strand, within argCat 4153.240 kb on - strand, within argCat 4153.244 kb on + strand, within argCat 4153.261 kb on + strand, within argCat 4153.298 kb on + strand, within argCat 4153.326 kb on - strand, within argCat 4153.348 kb on + strand, within argCat 4153.384 kb on + strand, within argCat 4153.384 kb on + strand, within argCat 4153.387 kb on - strand, within argCat 4153.390 kb on - strand, within argCat 4153.424 kb on + strand, within argCat 4153.425 kb on - strand, within argCat 4153.425 kb on - strand, within argCat 4153.440 kb on - strand, within argCat 4153.442 kb on - strand, within argCat 4153.501 kb on - strand, within argCat 4153.586 kb on + strand, within argCat 4153.586 kb on + strand, within argCat 4153.631 kb on + strand, within argCat 4153.638 kb on + strand, within argCat 4153.647 kb on + strand, within argCat 4153.647 kb on + strand, within argCat 4153.656 kb on - strand, within argCat 4153.656 kb on - strand, within argCat 4153.676 kb on + strand, within argCat 4153.684 kb on - strand, within argCat 4153.684 kb on - strandat 4153.694 kb on - strand, within argCat 4153.699 kb on - strand, within argCat 4153.699 kb on - strand, within argCat 4153.809 kb on + strand, within argCat 4153.825 kb on - strand, within argCat 4153.850 kb on - strand, within argCat 4153.852 kb on - strand, within argCat 4153.917 kb on + strand, within argCat 4153.917 kb on + strand, within argCat 4153.939 kb on + strandat 4153.939 kb on + strandat 4153.972 kb on + strandat 4154.018 kb on + strandat 4154.018 kb on + strandat 4154.023 kb on + strandat 4154.023 kb on + strandat 4154.023 kb on + strandat 4154.074 kb on + strandat 4154.079 kb on + strandat 4154.087 kb on - strandat 4154.090 kb on - strandat 4154.098 kb on - strandat 4154.121 kb on + strand, within argBat 4154.144 kb on + strand, within argBat 4154.153 kb on + strand, within argBat 4154.249 kb on + strand, within argBat 4154.249 kb on + strand, within argBat 4154.353 kb on + strand, within argBat 4154.353 kb on + strand, within argBat 4154.389 kb on + strand, within argBat 4154.394 kb on - strand, within argBat 4154.403 kb on + strand, within argBat 4154.421 kb on + strand, within argBat 4154.421 kb on + strand, within argBat 4154.472 kb on + strand, within argBat 4154.487 kb on - strand, within argBat 4154.497 kb on - strand, within argBat 4154.509 kb on + strand, within argBat 4154.517 kb on - strand, within argBat 4154.549 kb on - strand, within argBat 4154.659 kb on + strand, within argBat 4154.667 kb on - strand, within argBat 4154.667 kb on - strand, within argBat 4154.671 kb on - strand, within argBat 4154.683 kb on - strand, within argBat 4154.683 kb on - strand, within argBat 4154.696 kb on + strand, within argBat 4154.701 kb on + strand, within argBat 4154.707 kb on + strand, within argBat 4154.729 kb on + strand, within argBat 4154.729 kb on + strand, within argBat 4154.750 kb on + strandat 4154.750 kb on + strandat 4154.761 kb on + strandat 4154.761 kb on + strandat 4154.763 kb on + strandat 4154.763 kb on + strandat 4154.771 kb on - strandat 4154.773 kb on + strandat 4154.878 kb on - strandat 4154.900 kb on - strandat 4154.901 kb on + strandat 4154.968 kb on + strandat 4155.017 kb on + strand, within argHat 4155.019 kb on - strand, within argHat 4155.128 kb on + strand, within argHat 4155.128 kb on + strand, within argHat 4155.156 kb on - strand, within argHat 4155.178 kb on + strand, within argHat 4155.178 kb on + strand, within argHat 4155.193 kb on - strand, within argHat 4155.193 kb on - strand, within argHat 4155.229 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.237 kb on - strand, within argHat 4155.336 kb on - strand, within argHat 4155.338 kb on + strand, within argHat 4155.366 kb on - strand, within argHat 4155.366 kb on - strand, within argHat 4155.373 kb on + strand, within argHat 4155.381 kb on - strand, within argHat 4155.385 kb on - strand, within argHat 4155.436 kb on - strand, within argHat 4155.437 kb on + strand, within argHat 4155.437 kb on + strand, within argHat 4155.619 kb on - strand, within argHat 4155.699 kb on - strand, within argHat 4155.699 kb on - strand, within argHat 4155.713 kb on - strand, within argHat 4155.713 kb on - strand, within argHat 4155.767 kb on + strand, within argHat 4155.767 kb on + strand, within argHat 4155.770 kb on - strand, within argHat 4155.770 kb on - strand, within argH

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Per-strain Table

Position Strand Gene LocusTag Fraction 18 hr rep1_Tiberius_no_dilution
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4,153,072 - +0.2
4,153,072 - +0.6
4,153,082 + -0.2
4,153,082 + +0.2
4,153,087 - +0.3
4,153,101 + +0.1
4,153,137 - argC b3958 0.11 +0.3
4,153,184 - argC b3958 0.16 -0.4
4,153,208 - argC b3958 0.18 -0.2
4,153,208 - argC b3958 0.18 +0.1
4,153,240 - argC b3958 0.21 +0.2
4,153,240 - argC b3958 0.21 -0.2
4,153,244 + argC b3958 0.22 +0.3
4,153,261 + argC b3958 0.24 +0.0
4,153,298 + argC b3958 0.27 +2.0
4,153,326 - argC b3958 0.30 +0.2
4,153,348 + argC b3958 0.32 -0.5
4,153,384 + argC b3958 0.36 +0.1
4,153,384 + argC b3958 0.36 +0.0
4,153,387 - argC b3958 0.36 -0.3
4,153,390 - argC b3958 0.36 +0.6
4,153,424 + argC b3958 0.40 -1.1
4,153,425 - argC b3958 0.40 +0.4
4,153,425 - argC b3958 0.40 +0.8
4,153,440 - argC b3958 0.41 +0.3
4,153,442 - argC b3958 0.42 -0.1
4,153,501 - argC b3958 0.47 -0.0
4,153,586 + argC b3958 0.56 -0.4
4,153,586 + argC b3958 0.56 +0.4
4,153,631 + argC b3958 0.60 -0.6
4,153,638 + argC b3958 0.61 +0.5
4,153,647 + argC b3958 0.62 -0.4
4,153,647 + argC b3958 0.62 -0.7
4,153,656 - argC b3958 0.63 -0.4
4,153,656 - argC b3958 0.63 -0.4
4,153,676 + argC b3958 0.65 +0.0
4,153,684 - argC b3958 0.66 +0.1
4,153,684 - +0.4
4,153,694 - argC b3958 0.67 +0.4
4,153,699 - argC b3958 0.67 +0.3
4,153,699 - argC b3958 0.67 +0.3
4,153,809 + argC b3958 0.78 -0.4
4,153,825 - argC b3958 0.80 -0.6
4,153,850 - argC b3958 0.82 -0.1
4,153,852 - argC b3958 0.82 -0.1
4,153,917 + argC b3958 0.89 +0.4
4,153,917 + argC b3958 0.89 +0.4
4,153,939 + -0.4
4,153,939 + +0.0
4,153,972 + +0.3
4,154,018 + -0.3
4,154,018 + +0.2
4,154,023 + +0.3
4,154,023 + -0.8
4,154,023 + -0.6
4,154,074 + -0.7
4,154,079 + -0.3
4,154,087 - -0.3
4,154,090 - +0.0
4,154,098 - +0.4
4,154,121 + argB b3959 0.11 -1.0
4,154,144 + argB b3959 0.14 -0.1
4,154,153 + argB b3959 0.15 -0.2
4,154,249 + argB b3959 0.27 -1.6
4,154,249 + argB b3959 0.27 +0.2
4,154,353 + argB b3959 0.41 -1.5
4,154,353 + argB b3959 0.41 -1.0
4,154,389 + argB b3959 0.45 -0.2
4,154,394 - argB b3959 0.46 +0.1
4,154,403 + argB b3959 0.47 +0.0
4,154,421 + argB b3959 0.50 -0.2
4,154,421 + argB b3959 0.50 -0.0
4,154,472 + argB b3959 0.56 +0.3
4,154,487 - argB b3959 0.58 -0.4
4,154,497 - argB b3959 0.59 -0.2
4,154,509 + argB b3959 0.61 +0.2
4,154,517 - argB b3959 0.62 -0.2
4,154,549 - argB b3959 0.66 +0.2
4,154,659 + argB b3959 0.80 -0.1
4,154,667 - argB b3959 0.81 -1.1
4,154,667 - argB b3959 0.81 -0.3
4,154,671 - argB b3959 0.82 +0.1
4,154,683 - argB b3959 0.83 -0.2
4,154,683 - argB b3959 0.83 -0.0
4,154,696 + argB b3959 0.85 -0.3
4,154,701 + argB b3959 0.86 +0.4
4,154,707 + argB b3959 0.86 -0.5
4,154,729 + argB b3959 0.89 +0.2
4,154,729 + argB b3959 0.89 +0.6
4,154,750 + +0.8
4,154,750 + +1.3
4,154,761 + -0.7
4,154,761 + -0.3
4,154,763 + -0.4
4,154,763 + -0.0
4,154,771 - -0.2
4,154,773 + -0.9
4,154,878 - +0.1
4,154,900 - +1.2
4,154,901 + +0.5
4,154,968 + +0.5
4,155,017 + argH b3960 0.10 -1.8
4,155,019 - argH b3960 0.11 +0.0
4,155,128 + argH b3960 0.19 -1.1
4,155,128 + argH b3960 0.19 -0.1
4,155,156 - argH b3960 0.21 -0.1
4,155,178 + argH b3960 0.22 -0.2
4,155,178 + argH b3960 0.22 +0.1
4,155,193 - argH b3960 0.23 +0.6
4,155,193 - argH b3960 0.23 +1.2
4,155,229 - argH b3960 0.26 -0.0
4,155,237 - argH b3960 0.26 +0.2
4,155,237 - argH b3960 0.26 -0.3
4,155,237 - argH b3960 0.26 -2.4
4,155,336 - argH b3960 0.34 -0.3
4,155,338 + argH b3960 0.34 -0.6
4,155,366 - argH b3960 0.36 +0.3
4,155,366 - argH b3960 0.36 -0.2
4,155,373 + argH b3960 0.36 +0.4
4,155,381 - argH b3960 0.37 -0.7
4,155,385 - argH b3960 0.37 +0.5
4,155,436 - argH b3960 0.41 -0.0
4,155,437 + argH b3960 0.41 +0.3
4,155,437 + argH b3960 0.41 +0.3
4,155,619 - argH b3960 0.54 -0.8
4,155,699 - argH b3960 0.60 +0.3
4,155,699 - argH b3960 0.60 +1.0
4,155,713 - argH b3960 0.61 +0.3
4,155,713 - argH b3960 0.61 -0.2
4,155,767 + argH b3960 0.65 +0.0
4,155,767 + argH b3960 0.65 -0.7
4,155,770 - argH b3960 0.65 -0.2
4,155,770 - argH b3960 0.65 +0.7

Or see this region's nucleotide sequence