Strain Fitness in Escherichia coli BW25113 around b3365

Experiment: 18 hr rep1_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttsgA and nirB are separated by 261 nucleotidesnirB and nirD overlap by 4 nucleotidesnirD and nirC are separated by 125 nucleotides b3364: tsgA - hypothetical protein (NCBI), at 3,490,590 to 3,491,771 tsgA b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576 nirB b3366: nirD - nitrite reductase small subunit (NCBI), at 3,494,573 to 3,494,899 nirD b3367: nirC - nitrite transporter (NCBI), at 3,495,025 to 3,495,831 nirC Position (kb) 3492 3493 3494 3495Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3491.065 kb on + strand, within tsgAat 3491.065 kb on + strand, within tsgAat 3491.068 kb on - strand, within tsgAat 3491.068 kb on - strand, within tsgAat 3491.073 kb on - strand, within tsgAat 3491.073 kb on - strand, within tsgAat 3491.073 kb on - strand, within tsgAat 3491.075 kb on + strand, within tsgAat 3491.075 kb on + strand, within tsgAat 3491.075 kb on + strand, within tsgAat 3491.083 kb on - strand, within tsgAat 3491.179 kb on + strand, within tsgAat 3491.181 kb on + strand, within tsgAat 3491.234 kb on - strand, within tsgAat 3491.253 kb on - strand, within tsgAat 3491.253 kb on - strand, within tsgAat 3491.263 kb on - strand, within tsgAat 3491.274 kb on + strand, within tsgAat 3491.274 kb on + strand, within tsgAat 3491.280 kb on + strand, within tsgAat 3491.280 kb on + strand, within tsgAat 3491.304 kb on + strand, within tsgAat 3491.315 kb on + strand, within tsgAat 3491.367 kb on - strand, within tsgAat 3491.367 kb on - strand, within tsgAat 3491.375 kb on - strand, within tsgAat 3491.382 kb on - strand, within tsgAat 3491.402 kb on - strand, within tsgAat 3491.420 kb on + strand, within tsgAat 3491.420 kb on + strand, within tsgAat 3491.468 kb on + strand, within tsgAat 3491.470 kb on + strand, within tsgAat 3491.472 kb on + strand, within tsgAat 3491.517 kb on + strand, within tsgAat 3491.517 kb on + strand, within tsgAat 3491.517 kb on + strand, within tsgAat 3491.526 kb on + strand, within tsgAat 3491.540 kb on + strand, within tsgAat 3491.542 kb on + strand, within tsgAat 3491.542 kb on + strand, within tsgAat 3491.550 kb on - strand, within tsgAat 3491.550 kb on - strand, within tsgAat 3491.550 kb on - strand, within tsgAat 3491.554 kb on + strand, within tsgAat 3491.555 kb on - strand, within tsgAat 3491.603 kb on + strand, within tsgAat 3491.603 kb on + strand, within tsgAat 3491.621 kb on - strand, within tsgAat 3491.621 kb on - strand, within tsgAat 3491.646 kb on + strand, within tsgAat 3491.646 kb on + strand, within tsgAat 3491.646 kb on + strand, within tsgAat 3491.666 kb on - strandat 3491.675 kb on - strandat 3491.726 kb on - strandat 3491.726 kb on - strandat 3491.726 kb on - strandat 3491.747 kb on + strandat 3491.747 kb on + strandat 3491.755 kb on - strandat 3491.755 kb on - strandat 3491.801 kb on - strandat 3491.814 kb on + strandat 3491.814 kb on - strandat 3491.841 kb on + strandat 3491.841 kb on + strandat 3491.993 kb on + strandat 3491.993 kb on + strandat 3492.065 kb on + strandat 3492.065 kb on + strandat 3492.065 kb on + strandat 3492.073 kb on - strandat 3492.159 kb on + strandat 3492.159 kb on + strandat 3492.159 kb on + strandat 3492.167 kb on - strandat 3492.176 kb on - strandat 3492.198 kb on - strandat 3492.220 kb on - strandat 3492.275 kb on + strandat 3492.279 kb on + strandat 3492.316 kb on + strand, within nirBat 3492.319 kb on - strand, within nirBat 3492.352 kb on - strand, within nirBat 3492.355 kb on - strand, within nirBat 3492.361 kb on + strand, within nirBat 3492.424 kb on + strand, within nirBat 3492.424 kb on + strand, within nirBat 3492.434 kb on - strand, within nirBat 3492.444 kb on + strand, within nirBat 3492.495 kb on - strand, within nirBat 3492.495 kb on - strand, within nirBat 3492.574 kb on + strand, within nirBat 3492.574 kb on + strand, within nirBat 3492.599 kb on - strand, within nirBat 3492.647 kb on - strand, within nirBat 3492.677 kb on - strand, within nirBat 3492.680 kb on - strand, within nirBat 3492.690 kb on - strand, within nirBat 3492.765 kb on - strand, within nirBat 3492.765 kb on - strand, within nirBat 3492.792 kb on + strand, within nirBat 3492.793 kb on + strand, within nirBat 3492.822 kb on + strand, within nirBat 3492.822 kb on + strand, within nirBat 3492.918 kb on + strand, within nirBat 3492.918 kb on + strand, within nirBat 3492.952 kb on - strand, within nirBat 3492.959 kb on - strand, within nirBat 3492.970 kb on + strand, within nirBat 3492.979 kb on + strand, within nirBat 3493.051 kb on - strand, within nirBat 3493.053 kb on - strand, within nirBat 3493.141 kb on + strand, within nirBat 3493.146 kb on + strand, within nirBat 3493.149 kb on - strand, within nirBat 3493.149 kb on - strand, within nirBat 3493.179 kb on + strand, within nirBat 3493.265 kb on + strand, within nirBat 3493.284 kb on + strand, within nirBat 3493.292 kb on - strand, within nirBat 3493.299 kb on + strand, within nirBat 3493.299 kb on + strand, within nirBat 3493.312 kb on + strand, within nirBat 3493.312 kb on + strand, within nirBat 3493.347 kb on + strand, within nirBat 3493.352 kb on - strand, within nirBat 3493.358 kb on + strand, within nirBat 3493.364 kb on - strand, within nirBat 3493.364 kb on - strand, within nirBat 3493.414 kb on + strand, within nirBat 3493.422 kb on + strand, within nirBat 3493.422 kb on + strand, within nirBat 3493.430 kb on - strand, within nirBat 3493.430 kb on - strand, within nirBat 3493.432 kb on + strand, within nirBat 3493.481 kb on + strand, within nirBat 3493.582 kb on - strand, within nirBat 3493.591 kb on + strand, within nirBat 3493.599 kb on - strand, within nirBat 3493.623 kb on + strand, within nirBat 3493.623 kb on + strand, within nirBat 3493.623 kb on + strand, within nirBat 3493.724 kb on + strand, within nirBat 3493.779 kb on + strand, within nirBat 3493.794 kb on - strand, within nirBat 3493.805 kb on - strand, within nirBat 3493.805 kb on - strand, within nirBat 3493.811 kb on + strand, within nirBat 3493.882 kb on + strand, within nirBat 3493.882 kb on + strand, within nirBat 3493.959 kb on + strand, within nirBat 3493.989 kb on - strand, within nirBat 3494.041 kb on - strand, within nirBat 3494.107 kb on - strand, within nirBat 3494.107 kb on - strand, within nirBat 3494.247 kb on + strand, within nirBat 3494.347 kb on + strandat 3494.395 kb on + strandat 3494.434 kb on + strandat 3494.434 kb on + strandat 3494.452 kb on - strandat 3494.459 kb on + strandat 3494.488 kb on + strandat 3494.499 kb on - strandat 3494.499 kb on - strandat 3494.507 kb on - strandat 3494.507 kb on - strandat 3494.507 kb on - strandat 3494.540 kb on - strandat 3494.548 kb on + strandat 3494.564 kb on - strandat 3494.577 kb on - strandat 3494.577 kb on - strandat 3494.646 kb on - strand, within nirDat 3494.742 kb on - strand, within nirDat 3494.760 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.779 kb on - strand, within nirDat 3494.888 kb on - strandat 3494.888 kb on - strandat 3494.939 kb on + strandat 3494.939 kb on + strandat 3494.947 kb on - strandat 3494.947 kb on - strandat 3495.048 kb on + strandat 3495.056 kb on - strandat 3495.062 kb on - strandat 3495.071 kb on + strandat 3495.079 kb on - strandat 3495.113 kb on + strand, within nirCat 3495.113 kb on + strand, within nirCat 3495.123 kb on + strand, within nirCat 3495.124 kb on - strand, within nirCat 3495.124 kb on - strand, within nirCat 3495.129 kb on + strand, within nirCat 3495.129 kb on + strand, within nirCat 3495.131 kb on + strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.182 kb on + strand, within nirCat 3495.195 kb on - strand, within nirCat 3495.197 kb on + strand, within nirCat 3495.197 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.259 kb on - strand, within nirCat 3495.271 kb on - strand, within nirCat 3495.308 kb on + strand, within nirCat 3495.308 kb on + strand, within nirCat 3495.311 kb on - strand, within nirCat 3495.311 kb on - strand, within nirCat 3495.353 kb on - strand, within nirCat 3495.358 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.396 kb on - strand, within nirCat 3495.491 kb on + strand, within nirCat 3495.535 kb on + strand, within nirC

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Per-strain Table

Position Strand Gene LocusTag Fraction 18 hr rep1_Tiberius_no_dilution
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3,491,065 + tsgA b3364 0.40 -0.6
3,491,065 + tsgA b3364 0.40 -0.0
3,491,068 - tsgA b3364 0.40 -0.2
3,491,068 - tsgA b3364 0.40 -0.5
3,491,073 - tsgA b3364 0.41 +1.5
3,491,073 - tsgA b3364 0.41 +0.4
3,491,073 - tsgA b3364 0.41 +1.6
3,491,075 + tsgA b3364 0.41 +0.4
3,491,075 + tsgA b3364 0.41 +0.0
3,491,075 + tsgA b3364 0.41 +0.1
3,491,083 - tsgA b3364 0.42 -0.7
3,491,179 + tsgA b3364 0.50 +1.6
3,491,181 + tsgA b3364 0.50 +0.4
3,491,234 - tsgA b3364 0.54 +0.0
3,491,253 - tsgA b3364 0.56 -0.5
3,491,253 - tsgA b3364 0.56 -0.5
3,491,263 - tsgA b3364 0.57 -0.3
3,491,274 + tsgA b3364 0.58 -0.3
3,491,274 + tsgA b3364 0.58 -0.4
3,491,280 + tsgA b3364 0.58 +0.3
3,491,280 + tsgA b3364 0.58 +0.4
3,491,304 + tsgA b3364 0.60 +0.4
3,491,315 + tsgA b3364 0.61 +0.2
3,491,367 - tsgA b3364 0.66 +0.6
3,491,367 - tsgA b3364 0.66 -0.3
3,491,375 - tsgA b3364 0.66 +1.1
3,491,382 - tsgA b3364 0.67 -0.8
3,491,402 - tsgA b3364 0.69 -0.1
3,491,420 + tsgA b3364 0.70 +0.5
3,491,420 + tsgA b3364 0.70 +1.9
3,491,468 + tsgA b3364 0.74 +0.2
3,491,470 + tsgA b3364 0.74 -0.2
3,491,472 + tsgA b3364 0.75 +0.1
3,491,517 + tsgA b3364 0.78 +0.7
3,491,517 + tsgA b3364 0.78 -0.8
3,491,517 + tsgA b3364 0.78 +0.3
3,491,526 + tsgA b3364 0.79 +0.1
3,491,540 + tsgA b3364 0.80 +0.5
3,491,542 + tsgA b3364 0.81 +0.4
3,491,542 + tsgA b3364 0.81 -0.0
3,491,550 - tsgA b3364 0.81 -0.2
3,491,550 - tsgA b3364 0.81 +0.7
3,491,550 - tsgA b3364 0.81 +0.3
3,491,554 + tsgA b3364 0.82 -0.0
3,491,555 - tsgA b3364 0.82 -0.3
3,491,603 + tsgA b3364 0.86 -0.6
3,491,603 + tsgA b3364 0.86 +0.3
3,491,621 - tsgA b3364 0.87 -0.4
3,491,621 - tsgA b3364 0.87 -1.5
3,491,646 + tsgA b3364 0.89 +0.6
3,491,646 + tsgA b3364 0.89 -0.1
3,491,646 + tsgA b3364 0.89 +0.5
3,491,666 - -0.2
3,491,675 - +0.7
3,491,726 - -1.5
3,491,726 - -0.1
3,491,726 - +0.2
3,491,747 + +0.5
3,491,747 + -0.1
3,491,755 - -0.3
3,491,755 - +0.2
3,491,801 - +0.0
3,491,814 + -0.0
3,491,814 - -0.0
3,491,841 + +0.6
3,491,841 + +0.5
3,491,993 + -0.0
3,491,993 + +0.2
3,492,065 + +0.9
3,492,065 + -0.1
3,492,065 + +0.0
3,492,073 - -0.1
3,492,159 + -0.1
3,492,159 + -0.2
3,492,159 + -0.5
3,492,167 - +1.2
3,492,176 - +0.5
3,492,198 - +0.1
3,492,220 - +0.3
3,492,275 + -1.6
3,492,279 + +0.9
3,492,316 + nirB b3365 0.11 +0.2
3,492,319 - nirB b3365 0.11 -0.0
3,492,352 - nirB b3365 0.13 -0.0
3,492,355 - nirB b3365 0.13 -0.2
3,492,361 + nirB b3365 0.13 +0.5
3,492,424 + nirB b3365 0.15 +0.2
3,492,424 + nirB b3365 0.15 -1.1
3,492,434 - nirB b3365 0.16 +0.0
3,492,444 + nirB b3365 0.16 -0.3
3,492,495 - nirB b3365 0.18 -0.2
3,492,495 - nirB b3365 0.18 -0.7
3,492,574 + nirB b3365 0.21 +0.1
3,492,574 + nirB b3365 0.21 +0.1
3,492,599 - nirB b3365 0.22 -0.6
3,492,647 - nirB b3365 0.24 -0.2
3,492,677 - nirB b3365 0.25 +0.2
3,492,680 - nirB b3365 0.25 -0.0
3,492,690 - nirB b3365 0.26 -0.7
3,492,765 - nirB b3365 0.29 -0.4
3,492,765 - nirB b3365 0.29 -1.5
3,492,792 + nirB b3365 0.30 -0.6
3,492,793 + nirB b3365 0.30 -0.1
3,492,822 + nirB b3365 0.31 -0.0
3,492,822 + nirB b3365 0.31 +0.9
3,492,918 + nirB b3365 0.35 +0.5
3,492,918 + nirB b3365 0.35 +1.5
3,492,952 - nirB b3365 0.36 -0.2
3,492,959 - nirB b3365 0.36 +0.2
3,492,970 + nirB b3365 0.37 +0.1
3,492,979 + nirB b3365 0.37 -0.6
3,493,051 - nirB b3365 0.40 +0.4
3,493,053 - nirB b3365 0.40 +0.5
3,493,141 + nirB b3365 0.44 -0.8
3,493,146 + nirB b3365 0.44 +0.4
3,493,149 - nirB b3365 0.44 -0.2
3,493,149 - nirB b3365 0.44 +0.1
3,493,179 + nirB b3365 0.45 -1.3
3,493,265 + nirB b3365 0.48 +0.8
3,493,284 + nirB b3365 0.49 -0.2
3,493,292 - nirB b3365 0.49 -0.7
3,493,299 + nirB b3365 0.50 +0.3
3,493,299 + nirB b3365 0.50 -1.0
3,493,312 + nirB b3365 0.50 -1.3
3,493,312 + nirB b3365 0.50 -0.2
3,493,347 + nirB b3365 0.52 -0.0
3,493,352 - nirB b3365 0.52 -0.0
3,493,358 + nirB b3365 0.52 -0.5
3,493,364 - nirB b3365 0.52 -0.4
3,493,364 - nirB b3365 0.52 -0.2
3,493,414 + nirB b3365 0.54 -0.6
3,493,422 + nirB b3365 0.55 +0.8
3,493,422 + nirB b3365 0.55 -0.2
3,493,430 - nirB b3365 0.55 +1.1
3,493,430 - nirB b3365 0.55 -0.6
3,493,432 + nirB b3365 0.55 -0.2
3,493,481 + nirB b3365 0.57 +0.3
3,493,582 - nirB b3365 0.61 +0.1
3,493,591 + nirB b3365 0.61 -0.1
3,493,599 - nirB b3365 0.62 -0.4
3,493,623 + nirB b3365 0.62 -0.1
3,493,623 + nirB b3365 0.62 -0.1
3,493,623 + nirB b3365 0.62 -0.6
3,493,724 + nirB b3365 0.66 -1.4
3,493,779 + nirB b3365 0.69 -1.5
3,493,794 - nirB b3365 0.69 -0.4
3,493,805 - nirB b3365 0.70 +0.6
3,493,805 - nirB b3365 0.70 -1.2
3,493,811 + nirB b3365 0.70 -0.6
3,493,882 + nirB b3365 0.73 -0.3
3,493,882 + nirB b3365 0.73 +0.1
3,493,959 + nirB b3365 0.76 -0.8
3,493,989 - nirB b3365 0.77 -0.6
3,494,041 - nirB b3365 0.79 -0.6
3,494,107 - nirB b3365 0.82 +0.7
3,494,107 - nirB b3365 0.82 -0.2
3,494,247 + nirB b3365 0.87 +0.3
3,494,347 + +0.0
3,494,395 + -0.3
3,494,434 + +1.3
3,494,434 + -0.3
3,494,452 - +0.2
3,494,459 + +0.3
3,494,488 + +0.0
3,494,499 - -0.4
3,494,499 - -0.4
3,494,507 - +0.2
3,494,507 - -1.3
3,494,507 - +0.9
3,494,540 - +3.0
3,494,548 + +0.6
3,494,564 - -0.6
3,494,577 - -1.1
3,494,577 - +0.4
3,494,646 - nirD b3366 0.22 +0.3
3,494,742 - nirD b3366 0.52 +0.0
3,494,760 + nirD b3366 0.57 -0.7
3,494,765 + nirD b3366 0.59 -0.2
3,494,765 + nirD b3366 0.59 +0.2
3,494,765 + nirD b3366 0.59 +0.1
3,494,779 - nirD b3366 0.63 -0.3
3,494,888 - +0.7
3,494,888 - +0.6
3,494,939 + +0.1
3,494,939 + -0.1
3,494,947 - -0.4
3,494,947 - -0.5
3,495,048 + +0.1
3,495,056 - +0.1
3,495,062 - +0.8
3,495,071 + -0.8
3,495,079 - -0.4
3,495,113 + nirC b3367 0.11 -0.3
3,495,113 + nirC b3367 0.11 -2.1
3,495,123 + nirC b3367 0.12 -1.5
3,495,124 - nirC b3367 0.12 +0.7
3,495,124 - nirC b3367 0.12 -0.1
3,495,129 + nirC b3367 0.13 -0.6
3,495,129 + nirC b3367 0.13 -0.9
3,495,131 + nirC b3367 0.13 -0.3
3,495,148 - nirC b3367 0.15 +0.3
3,495,148 - nirC b3367 0.15 +1.1
3,495,148 - nirC b3367 0.15 +0.6
3,495,148 - nirC b3367 0.15 +1.0
3,495,182 + nirC b3367 0.19 +0.2
3,495,195 - nirC b3367 0.21 +0.2
3,495,197 + nirC b3367 0.21 -0.2
3,495,197 + nirC b3367 0.21 -0.3
3,495,251 + nirC b3367 0.28 +0.4
3,495,251 + nirC b3367 0.28 +0.4
3,495,251 + nirC b3367 0.28 +0.3
3,495,259 - nirC b3367 0.29 +0.4
3,495,271 - nirC b3367 0.30 -0.5
3,495,308 + nirC b3367 0.35 -0.2
3,495,308 + nirC b3367 0.35 +0.6
3,495,311 - nirC b3367 0.35 -0.8
3,495,311 - nirC b3367 0.35 +0.0
3,495,353 - nirC b3367 0.41 -0.4
3,495,358 + nirC b3367 0.41 +0.0
3,495,388 + nirC b3367 0.45 +0.5
3,495,388 + nirC b3367 0.45 -0.2
3,495,388 + nirC b3367 0.45 +0.2
3,495,388 + nirC b3367 0.45 +0.2
3,495,388 + nirC b3367 0.45 -0.8
3,495,396 - nirC b3367 0.46 +0.3
3,495,491 + nirC b3367 0.58 -0.9
3,495,535 + nirC b3367 0.63 +0.3

Or see this region's nucleotide sequence