Experiment: 18 hr rep1_HD100_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrlC and rrfC are separated by 92 nucleotides rrfC and aspT are separated by 52 nucleotides aspT and trpT are separated by 8 nucleotides trpT and hdfR are separated by 95 nucleotides
b3758: rrlC - 23S ribosomal RNA (NCBI), at 3,941,727 to 3,944,630
rrlC
b3759: rrfC - 5S ribosomal RNA (NCBI), at 3,944,723 to 3,944,842
rrfC
b3760: aspT - tRNA-Asp (NCBI), at 3,944,895 to 3,944,971
aspT
b3761: trpT - tRNA-Trp (NCBI), at 3,944,980 to 3,945,055
trpT
b4480: hdfR - transcriptional regulator HdfR (NCBI), at 3,945,151 to 3,945,990
hdfR
Position (kb)
3944
3945
3946 Strain fitness (log2 ratio)
-2
-1
0
1 at 3944.961 kb on + strand, within aspT at 3944.971 kb on + strand at 3945.046 kb on + strand, within trpT at 3945.298 kb on + strand, within hdfR at 3945.298 kb on + strand, within hdfR at 3945.322 kb on - strand, within hdfR at 3945.322 kb on - strand, within hdfR at 3945.390 kb on - strand, within hdfR at 3945.392 kb on + strand, within hdfR at 3945.392 kb on + strand, within hdfR at 3945.402 kb on - strand, within hdfR at 3945.402 kb on - strand, within hdfR at 3945.543 kb on + strand, within hdfR at 3945.543 kb on + strand, within hdfR at 3945.551 kb on - strand, within hdfR at 3945.744 kb on - strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.897 kb on + strand, within hdfR at 3945.902 kb on + strand, within hdfR at 3945.914 kb on - strand at 3946.030 kb on - strand at 3946.030 kb on - strand at 3946.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 18 hr rep1_HD100_no_dilution remove 3,944,961 + aspT b3760 0.86 -0.6 3,944,971 + +0.2 3,945,046 + trpT b3761 0.87 -0.8 3,945,298 + hdfR b4480 0.17 -0.7 3,945,298 + hdfR b4480 0.17 -2.4 3,945,322 - hdfR b4480 0.20 -0.5 3,945,322 - hdfR b4480 0.20 -0.7 3,945,390 - hdfR b4480 0.28 +0.3 3,945,392 + hdfR b4480 0.29 -0.6 3,945,392 + hdfR b4480 0.29 +0.4 3,945,402 - hdfR b4480 0.30 +0.7 3,945,402 - hdfR b4480 0.30 -0.7 3,945,543 + hdfR b4480 0.47 +1.2 3,945,543 + hdfR b4480 0.47 +0.9 3,945,551 - hdfR b4480 0.48 -0.4 3,945,744 - hdfR b4480 0.71 +1.0 3,945,783 + hdfR b4480 0.75 -0.6 3,945,783 + hdfR b4480 0.75 +0.0 3,945,897 + hdfR b4480 0.89 +0.7 3,945,902 + hdfR b4480 0.89 +0.2 3,945,914 - +0.1 3,946,030 - -0.6 3,946,030 - -0.7 3,946,045 + -0.0
Or see this region's nucleotide sequence