Strain Fitness in Escherichia coli BW25113 around b4004

Experiment: 18 hr NoPredatorControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntzraS and zraR overlap by 4 nucleotideszraR and purD overlap by 4 nucleotides b4003: zraS - sensory histidine kinase in two-component regulatory system with ZraR (NCBI), at 4,199,949 to 4,201,346 zraS b4004: zraR - fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein (NCBI), at 4,201,343 to 4,202,668 zraR b4005: purD - phosphoribosylamine--glycine ligase (NCBI), at 4,202,665 to 4,203,954 purD Position (kb) 4201 4202 4203Strain fitness (log2 ratio) -2 -1 0 1at 4200.378 kb on + strand, within zraSat 4200.398 kb on - strand, within zraSat 4200.431 kb on + strand, within zraSat 4200.441 kb on - strand, within zraSat 4200.441 kb on - strand, within zraSat 4200.525 kb on + strand, within zraSat 4200.525 kb on + strand, within zraSat 4200.530 kb on + strand, within zraSat 4200.532 kb on + strand, within zraSat 4200.533 kb on - strand, within zraSat 4200.586 kb on - strand, within zraSat 4200.647 kb on - strand, within zraSat 4200.647 kb on - strand, within zraSat 4200.755 kb on - strand, within zraSat 4200.757 kb on - strand, within zraSat 4200.801 kb on - strand, within zraSat 4200.902 kb on - strand, within zraSat 4200.965 kb on - strand, within zraSat 4200.965 kb on - strand, within zraSat 4201.008 kb on - strand, within zraSat 4201.008 kb on - strand, within zraSat 4201.013 kb on - strand, within zraSat 4201.013 kb on - strand, within zraSat 4201.013 kb on - strand, within zraSat 4201.023 kb on - strand, within zraSat 4201.023 kb on - strand, within zraSat 4201.028 kb on - strand, within zraSat 4201.051 kb on - strand, within zraSat 4201.150 kb on + strand, within zraSat 4201.150 kb on + strand, within zraSat 4201.250 kb on + strandat 4201.308 kb on - strandat 4201.313 kb on + strandat 4201.313 kb on + strandat 4201.362 kb on + strandat 4201.409 kb on + strandat 4201.428 kb on - strandat 4201.509 kb on + strand, within zraRat 4201.593 kb on - strand, within zraRat 4201.593 kb on - strand, within zraRat 4201.600 kb on + strand, within zraRat 4201.600 kb on + strand, within zraRat 4201.640 kb on - strand, within zraRat 4201.685 kb on - strand, within zraRat 4201.707 kb on + strand, within zraRat 4201.717 kb on + strand, within zraRat 4201.719 kb on + strand, within zraRat 4201.722 kb on - strand, within zraRat 4201.722 kb on - strand, within zraRat 4201.727 kb on - strand, within zraRat 4201.727 kb on - strand, within zraRat 4201.727 kb on - strand, within zraRat 4201.746 kb on + strand, within zraRat 4201.756 kb on + strand, within zraRat 4201.789 kb on - strand, within zraRat 4201.798 kb on + strand, within zraRat 4201.798 kb on + strand, within zraRat 4201.841 kb on + strand, within zraRat 4201.873 kb on + strand, within zraRat 4201.932 kb on + strand, within zraRat 4201.935 kb on - strand, within zraRat 4201.935 kb on - strand, within zraRat 4201.945 kb on + strand, within zraRat 4202.109 kb on + strand, within zraRat 4202.111 kb on + strand, within zraRat 4202.195 kb on - strand, within zraRat 4202.199 kb on + strand, within zraRat 4202.214 kb on - strand, within zraRat 4202.239 kb on - strand, within zraRat 4202.239 kb on - strand, within zraRat 4202.420 kb on - strand, within zraRat 4202.420 kb on - strand, within zraRat 4202.631 kb on - strandat 4202.758 kb on + strandat 4202.777 kb on - strandat 4202.783 kb on - strandat 4202.785 kb on - strandat 4202.785 kb on - strandat 4202.800 kb on + strand, within purDat 4202.852 kb on - strand, within purDat 4202.865 kb on + strand, within purDat 4202.904 kb on + strand, within purDat 4202.904 kb on + strand, within purDat 4202.905 kb on - strand, within purDat 4202.905 kb on - strand, within purDat 4202.993 kb on - strand, within purDat 4203.007 kb on + strand, within purDat 4203.030 kb on + strand, within purDat 4203.128 kb on + strand, within purDat 4203.128 kb on + strand, within purDat 4203.130 kb on + strand, within purDat 4203.143 kb on - strand, within purDat 4203.159 kb on + strand, within purDat 4203.235 kb on + strand, within purDat 4203.243 kb on - strand, within purDat 4203.360 kb on + strand, within purDat 4203.385 kb on + strand, within purDat 4203.428 kb on + strand, within purDat 4203.445 kb on + strand, within purDat 4203.445 kb on + strand, within purDat 4203.491 kb on - strand, within purDat 4203.491 kb on - strand, within purDat 4203.496 kb on + strand, within purDat 4203.505 kb on + strand, within purDat 4203.550 kb on - strand, within purDat 4203.657 kb on - strand, within purDat 4203.657 kb on - strand, within purD

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Per-strain Table

Position Strand Gene LocusTag Fraction 18 hr NoPredatorControl
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4,200,378 + zraS b4003 0.31 -0.4
4,200,398 - zraS b4003 0.32 -0.3
4,200,431 + zraS b4003 0.34 -0.2
4,200,441 - zraS b4003 0.35 -0.3
4,200,441 - zraS b4003 0.35 +0.4
4,200,525 + zraS b4003 0.41 +0.3
4,200,525 + zraS b4003 0.41 +0.5
4,200,530 + zraS b4003 0.42 -0.3
4,200,532 + zraS b4003 0.42 +0.1
4,200,533 - zraS b4003 0.42 -0.0
4,200,586 - zraS b4003 0.46 +0.1
4,200,647 - zraS b4003 0.50 +0.7
4,200,647 - zraS b4003 0.50 +0.6
4,200,755 - zraS b4003 0.58 +0.4
4,200,757 - zraS b4003 0.58 -0.7
4,200,801 - zraS b4003 0.61 -0.2
4,200,902 - zraS b4003 0.68 +0.4
4,200,965 - zraS b4003 0.73 -1.3
4,200,965 - zraS b4003 0.73 -1.3
4,201,008 - zraS b4003 0.76 +1.0
4,201,008 - zraS b4003 0.76 -1.3
4,201,013 - zraS b4003 0.76 -0.4
4,201,013 - zraS b4003 0.76 -0.8
4,201,013 - zraS b4003 0.76 +0.0
4,201,023 - zraS b4003 0.77 -0.1
4,201,023 - zraS b4003 0.77 +0.3
4,201,028 - zraS b4003 0.77 +0.3
4,201,051 - zraS b4003 0.79 +0.0
4,201,150 + zraS b4003 0.86 +0.2
4,201,150 + zraS b4003 0.86 +0.4
4,201,250 + -0.4
4,201,308 - -0.7
4,201,313 + +0.0
4,201,313 + +0.5
4,201,362 + -0.0
4,201,409 + -0.3
4,201,428 - -0.2
4,201,509 + zraR b4004 0.13 -0.8
4,201,593 - zraR b4004 0.19 -1.0
4,201,593 - zraR b4004 0.19 -0.2
4,201,600 + zraR b4004 0.19 -0.0
4,201,600 + zraR b4004 0.19 -0.5
4,201,640 - zraR b4004 0.22 -1.4
4,201,685 - zraR b4004 0.26 -0.2
4,201,707 + zraR b4004 0.27 -1.4
4,201,717 + zraR b4004 0.28 +0.3
4,201,719 + zraR b4004 0.28 +0.7
4,201,722 - zraR b4004 0.29 -0.9
4,201,722 - zraR b4004 0.29 +0.2
4,201,727 - zraR b4004 0.29 -1.3
4,201,727 - zraR b4004 0.29 +0.6
4,201,727 - zraR b4004 0.29 +0.4
4,201,746 + zraR b4004 0.30 -0.7
4,201,756 + zraR b4004 0.31 +0.4
4,201,789 - zraR b4004 0.34 -0.2
4,201,798 + zraR b4004 0.34 -2.0
4,201,798 + zraR b4004 0.34 -1.3
4,201,841 + zraR b4004 0.38 +0.8
4,201,873 + zraR b4004 0.40 -0.0
4,201,932 + zraR b4004 0.44 -0.4
4,201,935 - zraR b4004 0.45 -0.7
4,201,935 - zraR b4004 0.45 -0.4
4,201,945 + zraR b4004 0.45 -0.0
4,202,109 + zraR b4004 0.58 +0.4
4,202,111 + zraR b4004 0.58 +0.0
4,202,195 - zraR b4004 0.64 -0.5
4,202,199 + zraR b4004 0.65 +0.6
4,202,214 - zraR b4004 0.66 -1.1
4,202,239 - zraR b4004 0.68 -0.9
4,202,239 - zraR b4004 0.68 -0.5
4,202,420 - zraR b4004 0.81 -0.6
4,202,420 - zraR b4004 0.81 -1.2
4,202,631 - -0.4
4,202,758 + -0.4
4,202,777 - +0.2
4,202,783 - +0.8
4,202,785 - -0.2
4,202,785 - +0.5
4,202,800 + purD b4005 0.10 -0.2
4,202,852 - purD b4005 0.14 -1.3
4,202,865 + purD b4005 0.16 -1.2
4,202,904 + purD b4005 0.19 +1.0
4,202,904 + purD b4005 0.19 +0.8
4,202,905 - purD b4005 0.19 +0.2
4,202,905 - purD b4005 0.19 -0.4
4,202,993 - purD b4005 0.25 -0.7
4,203,007 + purD b4005 0.27 -0.1
4,203,030 + purD b4005 0.28 +0.1
4,203,128 + purD b4005 0.36 -0.3
4,203,128 + purD b4005 0.36 -0.4
4,203,130 + purD b4005 0.36 +0.0
4,203,143 - purD b4005 0.37 +0.2
4,203,159 + purD b4005 0.38 -0.2
4,203,235 + purD b4005 0.44 -0.4
4,203,243 - purD b4005 0.45 -0.0
4,203,360 + purD b4005 0.54 -0.0
4,203,385 + purD b4005 0.56 +0.2
4,203,428 + purD b4005 0.59 -0.6
4,203,445 + purD b4005 0.60 +0.2
4,203,445 + purD b4005 0.60 -0.4
4,203,491 - purD b4005 0.64 -0.1
4,203,491 - purD b4005 0.64 -0.5
4,203,496 + purD b4005 0.64 -0.0
4,203,505 + purD b4005 0.65 -0.4
4,203,550 - purD b4005 0.69 +0.6
4,203,657 - purD b4005 0.77 +0.6
4,203,657 - purD b4005 0.77 +0.2

Or see this region's nucleotide sequence