Experiment: 18 hr NoPredatorControl
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaE and paaF overlap by 4 nucleotides paaF and paaG overlap by 1 nucleotides paaG and paaH are separated by 1 nucleotides
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288
paaF
b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076
paaG
b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505
paaH
Position (kb)
1456
1457
1458 Strain fitness (log2 ratio)
-1
0
1 at 1455.315 kb on + strand, within paaE at 1455.392 kb on - strand, within paaE at 1455.429 kb on - strand at 1455.610 kb on + strand, within paaF at 1456.073 kb on + strand, within paaF at 1456.216 kb on - strand at 1456.216 kb on - strand at 1456.490 kb on + strand, within paaG at 1456.637 kb on + strand, within paaG at 1456.780 kb on - strand, within paaG at 1456.910 kb on + strand, within paaG at 1457.029 kb on + strand at 1457.029 kb on + strand at 1457.192 kb on + strand at 1457.264 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.668 kb on + strand, within paaH at 1457.750 kb on + strand, within paaH at 1457.822 kb on + strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.941 kb on + strand, within paaH at 1457.979 kb on + strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.061 kb on - strand, within paaH
Per-strain Table
Position Strand Gene LocusTag Fraction 18 hr NoPredatorControl remove 1,455,315 + paaE b1392 0.80 +0.4 1,455,392 - paaE b1392 0.88 +0.3 1,455,429 - -0.3 1,455,610 + paaF b1393 0.12 -0.5 1,456,073 + paaF b1393 0.72 +0.2 1,456,216 - +0.6 1,456,216 - +0.8 1,456,490 + paaG b1394 0.26 -0.0 1,456,637 + paaG b1394 0.44 +0.2 1,456,780 - paaG b1394 0.62 +1.0 1,456,910 + paaG b1394 0.79 +0.4 1,457,029 + +0.8 1,457,029 + +0.6 1,457,192 + +0.7 1,457,264 + paaH b1395 0.13 +0.2 1,457,625 + paaH b1395 0.38 -0.6 1,457,625 + paaH b1395 0.38 -1.0 1,457,668 + paaH b1395 0.41 +0.5 1,457,750 + paaH b1395 0.47 -0.1 1,457,822 + paaH b1395 0.52 +0.2 1,457,921 - paaH b1395 0.59 +0.0 1,457,921 - paaH b1395 0.59 +0.0 1,457,941 + paaH b1395 0.60 +0.1 1,457,979 + paaH b1395 0.63 +1.0 1,458,061 - paaH b1395 0.69 -0.3 1,458,061 - paaH b1395 0.69 +0.5
Or see this region's nucleotide sequence