Experiment: 3 hr rep1_Tiberius_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serU and b1976 are separated by 93 nucleotides b1976 and asnT are separated by 100 nucleotides asnT and yeeJ are separated by 313 nucleotides
b1975: serU - tRNA-Ser (NCBI), at 2,041,492 to 2,041,581
serU
b1976: b1976 - orf, hypothetical protein (VIMSS), at 2,041,675 to 2,042,472
b1976
b1977: asnT - tRNA-Asn (NCBI), at 2,042,573 to 2,042,648
asnT
b1978: yeeJ - adhesin (RefSeq), at 2,042,962 to 2,050,038
yeeJ
Position (kb)
2042
2043 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2041.871 kb on + strand, within b1976 at 2041.999 kb on - strand, within b1976 at 2042.029 kb on + strand, within b1976 at 2042.037 kb on - strand, within b1976 at 2042.042 kb on + strand, within b1976 at 2042.055 kb on - strand, within b1976 at 2042.095 kb on + strand, within b1976 at 2042.160 kb on - strand, within b1976 at 2042.160 kb on - strand, within b1976 at 2042.403 kb on + strand at 2042.418 kb on - strand at 2042.434 kb on + strand at 2042.434 kb on + strand at 2042.619 kb on + strand, within asnT at 2042.619 kb on + strand, within asnT at 2042.747 kb on + strand at 2042.824 kb on - strand at 2042.917 kb on + strand at 2042.917 kb on + strand at 2042.923 kb on - strand at 2042.923 kb on - strand at 2042.923 kb on - strand at 2042.925 kb on - strand at 2042.963 kb on + strand at 2042.963 kb on + strand at 2042.972 kb on - strand at 2043.094 kb on + strand at 2043.094 kb on + strand at 2043.118 kb on + strand at 2043.176 kb on + strand at 2043.184 kb on - strand at 2043.319 kb on - strand at 2043.354 kb on + strand at 2043.432 kb on - strand at 2043.524 kb on + strand at 2043.524 kb on + strand at 2043.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_100fold_dilution remove 2,041,871 + b1976 b1976 0.25 -0.6 2,041,999 - b1976 b1976 0.41 +0.8 2,042,029 + b1976 b1976 0.44 -0.1 2,042,037 - b1976 b1976 0.45 +0.2 2,042,042 + b1976 b1976 0.46 -1.1 2,042,055 - b1976 b1976 0.48 +1.2 2,042,095 + b1976 b1976 0.53 +0.5 2,042,160 - b1976 b1976 0.61 +0.8 2,042,160 - b1976 b1976 0.61 -0.3 2,042,403 + -0.3 2,042,418 - -1.0 2,042,434 + +0.8 2,042,434 + +2.4 2,042,619 + asnT b1977 0.61 -1.2 2,042,619 + asnT b1977 0.61 -0.2 2,042,747 + -2.0 2,042,824 - +1.2 2,042,917 + -0.9 2,042,917 + -1.1 2,042,923 - -0.0 2,042,923 - +0.3 2,042,923 - +0.8 2,042,925 - -1.7 2,042,963 + -0.6 2,042,963 + +0.1 2,042,972 - +1.5 2,043,094 + +0.8 2,043,094 + -1.1 2,043,118 + +0.8 2,043,176 + -0.3 2,043,184 - +0.9 2,043,319 - +1.0 2,043,354 + +1.0 2,043,432 - -1.2 2,043,524 + +0.1 2,043,524 + +0.9 2,043,564 + -0.5
Or see this region's nucleotide sequence