Experiment: 3 hr rep1_Tiberius_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt marR and marA are separated by 19 nucleotides marA and marB are separated by 31 nucleotides marB and ydeD are separated by 30 nucleotides ydeD and ydeE are separated by 194 nucleotides
b1530: marR - multiple antibiotic resistance protein; repressor of mar operon (VIMSS), at 1,617,144 to 1,617,578
marR
b1531: marA - multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS), at 1,617,598 to 1,617,981
marA
b1532: marB - hypothetical protein (NCBI), at 1,618,013 to 1,618,231
marB
b1533: ydeD - orf, hypothetical protein (VIMSS), at 1,618,262 to 1,619,161
ydeD
b1534: ydeE - predicted transporter (NCBI), at 1,619,356 to 1,620,543
ydeE
Position (kb)
1618
1619
1620 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1617.277 kb on - strand, within marR at 1617.288 kb on + strand, within marR at 1617.291 kb on - strand, within marR at 1617.291 kb on - strand, within marR at 1617.302 kb on + strand, within marR at 1617.312 kb on - strand, within marR at 1617.358 kb on + strand, within marR at 1617.377 kb on + strand, within marR at 1617.377 kb on + strand, within marR at 1617.471 kb on - strand, within marR at 1617.606 kb on + strand at 1617.638 kb on + strand, within marA at 1617.655 kb on - strand, within marA at 1617.702 kb on + strand, within marA at 1618.111 kb on - strand, within marB at 1618.215 kb on - strand at 1618.234 kb on - strand at 1618.521 kb on - strand, within ydeD at 1618.521 kb on - strand, within ydeD at 1618.653 kb on + strand, within ydeD at 1618.995 kb on + strand, within ydeD at 1618.995 kb on + strand, within ydeD at 1619.282 kb on - strand at 1619.420 kb on + strand at 1619.420 kb on + strand at 1619.466 kb on + strand at 1619.668 kb on + strand, within ydeE at 1619.759 kb on + strand, within ydeE at 1619.768 kb on + strand, within ydeE at 1619.780 kb on + strand, within ydeE at 1619.872 kb on - strand, within ydeE at 1619.929 kb on + strand, within ydeE at 1619.929 kb on + strand, within ydeE at 1620.034 kb on - strand, within ydeE at 1620.095 kb on + strand, within ydeE
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_100fold_dilution remove 1,617,277 - marR b1530 0.31 +1.2 1,617,288 + marR b1530 0.33 +0.7 1,617,291 - marR b1530 0.34 -0.7 1,617,291 - marR b1530 0.34 +0.7 1,617,302 + marR b1530 0.36 +0.7 1,617,312 - marR b1530 0.39 -0.4 1,617,358 + marR b1530 0.49 +0.8 1,617,377 + marR b1530 0.54 +2.5 1,617,377 + marR b1530 0.54 -1.6 1,617,471 - marR b1530 0.75 +0.4 1,617,606 + +0.5 1,617,638 + marA b1531 0.10 +0.8 1,617,655 - marA b1531 0.15 -0.9 1,617,702 + marA b1531 0.27 +0.9 1,618,111 - marB b1532 0.45 -0.1 1,618,215 - +0.2 1,618,234 - +0.5 1,618,521 - ydeD b1533 0.29 -1.1 1,618,521 - ydeD b1533 0.29 -0.3 1,618,653 + ydeD b1533 0.43 -2.7 1,618,995 + ydeD b1533 0.81 +0.4 1,618,995 + ydeD b1533 0.81 -0.7 1,619,282 - +1.0 1,619,420 + -0.4 1,619,420 + -0.1 1,619,466 + +0.7 1,619,668 + ydeE b1534 0.26 -1.4 1,619,759 + ydeE b1534 0.34 +0.1 1,619,768 + ydeE b1534 0.35 +0.8 1,619,780 + ydeE b1534 0.36 +0.3 1,619,872 - ydeE b1534 0.43 +0.0 1,619,929 + ydeE b1534 0.48 -0.9 1,619,929 + ydeE b1534 0.48 +2.0 1,620,034 - ydeE b1534 0.57 +0.7 1,620,095 + ydeE b1534 0.62 +0.1
Or see this region's nucleotide sequence