Experiment: 3 hr rep1_Tiberius_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcK and tehA are separated by 123 nucleotides tehA and tehB overlap by 4 nucleotides tehB and ydcL are separated by 301 nucleotides
b1428: ydcK - predicted enzyme (NCBI), at 1,497,493 to 1,498,473
ydcK
b1429: tehA - potassium-tellurite ethidium and proflavin transporter (NCBI), at 1,498,597 to 1,499,589
tehA
b1430: tehB - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,499,586 to 1,500,179
tehB
b1431: ydcL - predicted lipoprotein (NCBI), at 1,500,481 to 1,501,149
ydcL
Position (kb)
1498
1499
1500 Strain fitness (log2 ratio)
-2
-1
0
1 at 1497.636 kb on + strand, within ydcK at 1497.662 kb on + strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.765 kb on + strand, within ydcK at 1497.766 kb on + strand, within ydcK at 1497.792 kb on - strand, within ydcK at 1497.805 kb on + strand, within ydcK at 1497.829 kb on - strand, within ydcK at 1498.038 kb on + strand, within ydcK at 1498.172 kb on + strand, within ydcK at 1498.172 kb on + strand, within ydcK at 1498.189 kb on - strand, within ydcK at 1498.189 kb on - strand, within ydcK at 1498.219 kb on - strand, within ydcK at 1498.471 kb on - strand at 1498.779 kb on - strand, within tehA at 1499.017 kb on - strand, within tehA at 1499.151 kb on + strand, within tehA at 1499.640 kb on + strand at 1499.650 kb on - strand, within tehB at 1499.699 kb on - strand, within tehB at 1499.709 kb on - strand, within tehB at 1499.726 kb on + strand, within tehB at 1499.741 kb on - strand, within tehB at 1499.831 kb on - strand, within tehB at 1499.859 kb on - strand, within tehB at 1499.991 kb on + strand, within tehB at 1499.991 kb on + strand, within tehB at 1500.069 kb on + strand, within tehB at 1500.069 kb on + strand, within tehB at 1500.113 kb on - strand, within tehB at 1500.128 kb on + strand at 1500.128 kb on + strand at 1500.191 kb on - strand at 1500.191 kb on - strand at 1500.496 kb on - strand at 1500.496 kb on - strand at 1500.540 kb on - strand at 1500.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_100fold_dilution remove 1,497,636 + ydcK b1428 0.15 +0.6 1,497,662 + ydcK b1428 0.17 -0.3 1,497,751 - ydcK b1428 0.26 +1.5 1,497,751 - ydcK b1428 0.26 +0.1 1,497,751 - ydcK b1428 0.26 -0.9 1,497,765 + ydcK b1428 0.28 -0.8 1,497,766 + ydcK b1428 0.28 -0.3 1,497,792 - ydcK b1428 0.30 +0.3 1,497,805 + ydcK b1428 0.32 +0.3 1,497,829 - ydcK b1428 0.34 -0.4 1,498,038 + ydcK b1428 0.56 -0.0 1,498,172 + ydcK b1428 0.69 +0.7 1,498,172 + ydcK b1428 0.69 +1.5 1,498,189 - ydcK b1428 0.71 -0.0 1,498,189 - ydcK b1428 0.71 -1.0 1,498,219 - ydcK b1428 0.74 +1.4 1,498,471 - +1.2 1,498,779 - tehA b1429 0.18 -2.1 1,499,017 - tehA b1429 0.42 -2.0 1,499,151 + tehA b1429 0.56 +0.6 1,499,640 + -2.6 1,499,650 - tehB b1430 0.11 +0.4 1,499,699 - tehB b1430 0.19 -0.4 1,499,709 - tehB b1430 0.21 -1.1 1,499,726 + tehB b1430 0.24 +1.5 1,499,741 - tehB b1430 0.26 -2.0 1,499,831 - tehB b1430 0.41 +0.3 1,499,859 - tehB b1430 0.46 +0.5 1,499,991 + tehB b1430 0.68 -0.4 1,499,991 + tehB b1430 0.68 +0.3 1,500,069 + tehB b1430 0.81 +1.3 1,500,069 + tehB b1430 0.81 +0.1 1,500,113 - tehB b1430 0.89 +0.5 1,500,128 + +1.3 1,500,128 + +0.0 1,500,191 - -2.1 1,500,191 - +0.8 1,500,496 - +0.5 1,500,496 - -0.1 1,500,540 - +1.5 1,500,542 - +0.8
Or see this region's nucleotide sequence