Experiment: 3 hr rep1_Tiberius_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt torA and torD overlap by 4 nucleotides torD and cbpM are separated by 151 nucleotides cbpM and cbpA overlap by 1 nucleotides
b0997: torA - trimethylamine N-oxide (TMAO) reductase I, catalytic subunit (NCBI), at 1,058,479 to 1,061,025
torA
b0998: torD - chaperone protein TorD (NCBI), at 1,061,022 to 1,061,621
torD
b0999: cbpM - modulator of CbpA co-chaperone (NCBI), at 1,061,773 to 1,062,078
cbpM
b1000: cbpA - curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI), at 1,062,078 to 1,062,998
cbpA
Position (kb)
1061
1062 Strain fitness (log2 ratio)
-1
0
1
2 at 1060.234 kb on - strand, within torA at 1060.234 kb on - strand, within torA at 1060.302 kb on - strand, within torA at 1060.302 kb on - strand, within torA at 1060.512 kb on + strand, within torA at 1060.744 kb on - strand, within torA at 1060.754 kb on + strand, within torA at 1060.754 kb on + strand, within torA at 1060.803 kb on + strand at 1060.826 kb on - strand at 1060.826 kb on - strand at 1060.847 kb on - strand at 1060.940 kb on + strand at 1060.989 kb on + strand at 1061.073 kb on + strand at 1061.083 kb on - strand, within torD at 1061.747 kb on - strand at 1061.789 kb on - strand at 1061.879 kb on - strand, within cbpM at 1061.889 kb on - strand, within cbpM at 1061.978 kb on - strand, within cbpM at 1061.978 kb on - strand, within cbpM at 1062.190 kb on + strand, within cbpA at 1062.191 kb on - strand, within cbpA at 1062.191 kb on - strand, within cbpA at 1062.401 kb on - strand, within cbpA at 1062.545 kb on - strand, within cbpA at 1062.572 kb on + strand, within cbpA
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_100fold_dilution remove 1,060,234 - torA b0997 0.69 -0.7 1,060,234 - torA b0997 0.69 +2.1 1,060,302 - torA b0997 0.72 +0.5 1,060,302 - torA b0997 0.72 -0.4 1,060,512 + torA b0997 0.80 +0.0 1,060,744 - torA b0997 0.89 +1.6 1,060,754 + torA b0997 0.89 -0.9 1,060,754 + torA b0997 0.89 +1.4 1,060,803 + +0.3 1,060,826 - -0.6 1,060,826 - -0.7 1,060,847 - +0.2 1,060,940 + +0.7 1,060,989 + -0.5 1,061,073 + +0.6 1,061,083 - torD b0998 0.10 -1.1 1,061,747 - +0.1 1,061,789 - +0.3 1,061,879 - cbpM b0999 0.35 +0.2 1,061,889 - cbpM b0999 0.38 +0.2 1,061,978 - cbpM b0999 0.67 +0.2 1,061,978 - cbpM b0999 0.67 +0.9 1,062,190 + cbpA b1000 0.12 +0.7 1,062,191 - cbpA b1000 0.12 +0.2 1,062,191 - cbpA b1000 0.12 +1.1 1,062,401 - cbpA b1000 0.35 +0.5 1,062,545 - cbpA b1000 0.51 +0.0 1,062,572 + cbpA b1000 0.54 -1.2
Or see this region's nucleotide sequence