Experiment: 3 hr rep1_Tiberius_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fixC and fixX overlap by 4 nucleotides fixX and yaaU are separated by 56 nucleotides
b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466
fixC
b0044: fixX - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 45,463 to 45,750
fixX
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
Position (kb) 45
46 Strain fitness (log2 ratio) -2
-1
0
1
2 at 44.581 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.649 kb on + strand, within fixC at 44.675 kb on - strand, within fixC at 44.676 kb on - strand, within fixC at 44.765 kb on + strand, within fixC at 44.857 kb on - strand, within fixC at 44.862 kb on - strand, within fixC at 44.906 kb on - strand, within fixC at 45.034 kb on + strand, within fixC at 45.034 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.232 kb on + strand, within fixC at 45.241 kb on - strand, within fixC at 45.323 kb on - strand, within fixC at 45.339 kb on + strand at 45.339 kb on + strand at 45.340 kb on - strand at 45.426 kb on + strand at 45.546 kb on - strand, within fixX at 45.610 kb on + strand, within fixX at 45.700 kb on - strand, within fixX at 45.704 kb on + strand, within fixX at 45.704 kb on + strand, within fixX at 45.844 kb on + strand at 45.880 kb on - strand at 45.924 kb on + strand at 46.113 kb on + strand, within yaaU at 46.113 kb on + strand, within yaaU at 46.142 kb on - strand, within yaaU at 46.144 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.176 kb on - strand, within yaaU at 46.240 kb on + strand, within yaaU at 46.284 kb on - strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.575 kb on + strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.707 kb on - strand, within yaaU
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_100fold_dilution remove 44,581 - fixC b0043 0.31 -0.0 44,622 - fixC b0043 0.34 +0.0 44,622 - fixC b0043 0.34 +0.0 44,649 + fixC b0043 0.36 -0.5 44,675 - fixC b0043 0.38 +0.3 44,676 - fixC b0043 0.39 -1.3 44,765 + fixC b0043 0.45 +0.3 44,857 - fixC b0043 0.53 +0.3 44,862 - fixC b0043 0.53 -1.4 44,906 - fixC b0043 0.56 +1.6 45,034 + fixC b0043 0.66 +1.4 45,034 + fixC b0043 0.66 -2.5 45,044 + fixC b0043 0.67 -0.5 45,044 + fixC b0043 0.67 +0.3 45,232 + fixC b0043 0.82 -0.6 45,241 - fixC b0043 0.82 -0.4 45,323 - fixC b0043 0.89 -0.2 45,339 + -0.7 45,339 + -0.3 45,340 - -0.5 45,426 + +0.9 45,546 - fixX b0044 0.29 +0.2 45,610 + fixX b0044 0.51 -0.1 45,700 - fixX b0044 0.82 -1.1 45,704 + fixX b0044 0.84 -1.2 45,704 + fixX b0044 0.84 -1.2 45,844 + +0.7 45,880 - +2.1 45,924 + +0.9 46,113 + yaaU b0045 0.23 +0.5 46,113 + yaaU b0045 0.23 +0.0 46,142 - yaaU b0045 0.25 -0.6 46,144 - yaaU b0045 0.25 +0.9 46,176 - yaaU b0045 0.28 -1.4 46,176 - yaaU b0045 0.28 +0.7 46,240 + yaaU b0045 0.33 -0.9 46,284 - yaaU b0045 0.36 -0.2 46,329 + yaaU b0045 0.39 -0.1 46,329 + yaaU b0045 0.39 +0.9 46,463 + yaaU b0045 0.49 -2.0 46,463 + yaaU b0045 0.49 +1.3 46,564 + yaaU b0045 0.57 -0.7 46,564 + yaaU b0045 0.57 -0.7 46,575 + yaaU b0045 0.58 -0.6 46,707 - yaaU b0045 0.68 +0.1 46,707 - yaaU b0045 0.68 +1.9
Or see this region's nucleotide sequence