Experiment: 3 hr rep1_Tiberius_10fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygcF and yqcG are separated by 138 nucleotides yqcG and ygcG are separated by 13 nucleotides ygcG and eno are separated by 59 nucleotides
b2777: ygcF - hypothetical protein (NCBI), at 2,902,769 to 2,903,440
ygcF
b4682: yqcG - expressed protein (RefSeq), at 2,903,579 to 2,903,719
yqcG
b2778: ygcG - orf, hypothetical protein (VIMSS), at 2,903,733 to 2,904,605
ygcG
b2779: eno - phosphopyruvate hydratase (NCBI), at 2,904,665 to 2,905,963
eno
Position (kb)
2903
2904
2905 Strain fitness (log2 ratio)
-2
-1
0
1 at 2902.735 kb on - strand at 2902.746 kb on + strand at 2902.770 kb on - strand at 2902.770 kb on - strand at 2902.797 kb on - strand at 2902.817 kb on - strand at 2902.832 kb on - strand at 2902.832 kb on - strand at 2902.846 kb on - strand, within ygcF at 2902.852 kb on - strand, within ygcF at 2902.852 kb on - strand, within ygcF at 2902.859 kb on - strand, within ygcF at 2902.876 kb on + strand, within ygcF at 2902.882 kb on - strand, within ygcF at 2902.887 kb on + strand, within ygcF at 2902.887 kb on - strand, within ygcF at 2902.887 kb on - strand, within ygcF at 2902.887 kb on - strand, within ygcF at 2902.887 kb on - strand, within ygcF at 2902.917 kb on - strand, within ygcF at 2902.917 kb on - strand, within ygcF at 2902.950 kb on - strand, within ygcF at 2902.981 kb on + strand, within ygcF at 2902.990 kb on - strand, within ygcF at 2902.990 kb on - strand, within ygcF at 2903.002 kb on + strand, within ygcF at 2903.010 kb on - strand, within ygcF at 2903.010 kb on - strand, within ygcF at 2903.015 kb on + strand, within ygcF at 2903.034 kb on - strand, within ygcF at 2903.050 kb on - strand, within ygcF at 2903.050 kb on - strand, within ygcF at 2903.058 kb on + strand, within ygcF at 2903.058 kb on + strand, within ygcF at 2903.074 kb on - strand, within ygcF at 2903.074 kb on - strand, within ygcF at 2903.187 kb on + strand, within ygcF at 2903.235 kb on + strand, within ygcF at 2903.307 kb on + strand, within ygcF at 2903.328 kb on + strand, within ygcF at 2903.332 kb on + strand, within ygcF at 2903.332 kb on + strand, within ygcF at 2903.335 kb on - strand, within ygcF at 2903.340 kb on - strand, within ygcF at 2903.388 kb on + strand at 2903.388 kb on + strand at 2903.394 kb on + strand at 2903.394 kb on + strand at 2903.396 kb on + strand at 2903.409 kb on + strand at 2903.416 kb on - strand at 2903.417 kb on - strand at 2903.417 kb on - strand at 2903.417 kb on - strand at 2903.437 kb on + strand at 2903.445 kb on - strand at 2903.448 kb on + strand at 2903.471 kb on - strand at 2903.503 kb on + strand at 2903.510 kb on + strand at 2903.525 kb on + strand at 2904.015 kb on - strand, within ygcG at 2904.017 kb on + strand, within ygcG at 2904.017 kb on - strand, within ygcG at 2904.027 kb on - strand, within ygcG at 2904.045 kb on + strand, within ygcG at 2904.061 kb on + strand, within ygcG at 2904.107 kb on + strand, within ygcG at 2904.113 kb on + strand, within ygcG at 2904.167 kb on + strand, within ygcG at 2904.205 kb on + strand, within ygcG at 2904.209 kb on + strand, within ygcG at 2904.439 kb on - strand, within ygcG at 2904.485 kb on - strand, within ygcG at 2904.485 kb on - strand, within ygcG at 2904.485 kb on - strand, within ygcG at 2904.492 kb on + strand, within ygcG at 2904.503 kb on - strand, within ygcG at 2904.503 kb on - strand, within ygcG at 2904.513 kb on + strand, within ygcG at 2904.525 kb on + strand at 2904.528 kb on + strand at 2904.573 kb on + strand at 2904.603 kb on - strand at 2904.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_10fold_dilution remove 2,902,735 - +1.7 2,902,746 + +1.7 2,902,770 - +0.1 2,902,770 - -0.5 2,902,797 - -0.7 2,902,817 - -0.4 2,902,832 - +0.8 2,902,832 - +0.4 2,902,846 - ygcF b2777 0.11 +0.3 2,902,852 - ygcF b2777 0.12 +0.5 2,902,852 - ygcF b2777 0.12 -0.1 2,902,859 - ygcF b2777 0.13 -0.1 2,902,876 + ygcF b2777 0.16 +0.7 2,902,882 - ygcF b2777 0.17 +0.4 2,902,887 + ygcF b2777 0.18 +0.4 2,902,887 - ygcF b2777 0.18 -0.0 2,902,887 - ygcF b2777 0.18 +0.1 2,902,887 - ygcF b2777 0.18 +0.8 2,902,887 - ygcF b2777 0.18 +0.3 2,902,917 - ygcF b2777 0.22 +0.0 2,902,917 - ygcF b2777 0.22 +0.7 2,902,950 - ygcF b2777 0.27 +0.1 2,902,981 + ygcF b2777 0.32 +0.4 2,902,990 - ygcF b2777 0.33 +0.3 2,902,990 - ygcF b2777 0.33 +0.1 2,903,002 + ygcF b2777 0.35 +0.1 2,903,010 - ygcF b2777 0.36 +0.4 2,903,010 - ygcF b2777 0.36 +0.7 2,903,015 + ygcF b2777 0.37 -0.1 2,903,034 - ygcF b2777 0.39 +0.7 2,903,050 - ygcF b2777 0.42 +0.2 2,903,050 - ygcF b2777 0.42 +0.5 2,903,058 + ygcF b2777 0.43 -0.3 2,903,058 + ygcF b2777 0.43 -1.5 2,903,074 - ygcF b2777 0.45 +0.0 2,903,074 - ygcF b2777 0.45 -0.2 2,903,187 + ygcF b2777 0.62 -0.3 2,903,235 + ygcF b2777 0.69 -1.7 2,903,307 + ygcF b2777 0.80 -0.2 2,903,328 + ygcF b2777 0.83 +0.2 2,903,332 + ygcF b2777 0.84 +0.0 2,903,332 + ygcF b2777 0.84 -1.1 2,903,335 - ygcF b2777 0.84 -0.2 2,903,340 - ygcF b2777 0.85 -0.2 2,903,388 + -0.1 2,903,388 + -0.9 2,903,394 + -0.1 2,903,394 + -1.0 2,903,396 + -0.1 2,903,409 + -0.6 2,903,416 - -1.1 2,903,417 - +0.4 2,903,417 - -0.3 2,903,417 - -0.1 2,903,437 + -1.8 2,903,445 - +0.1 2,903,448 + -0.7 2,903,471 - -0.9 2,903,503 + -0.5 2,903,510 + -0.8 2,903,525 + -0.8 2,904,015 - ygcG b2778 0.32 +1.7 2,904,017 + ygcG b2778 0.33 +1.7 2,904,017 - ygcG b2778 0.33 +1.7 2,904,027 - ygcG b2778 0.34 +1.7 2,904,045 + ygcG b2778 0.36 +1.7 2,904,061 + ygcG b2778 0.38 +0.1 2,904,107 + ygcG b2778 0.43 +0.7 2,904,113 + ygcG b2778 0.44 -0.9 2,904,167 + ygcG b2778 0.50 -0.3 2,904,205 + ygcG b2778 0.54 +1.7 2,904,209 + ygcG b2778 0.55 -0.9 2,904,439 - ygcG b2778 0.81 -0.8 2,904,485 - ygcG b2778 0.86 -0.6 2,904,485 - ygcG b2778 0.86 -0.8 2,904,485 - ygcG b2778 0.86 -0.4 2,904,492 + ygcG b2778 0.87 -0.1 2,904,503 - ygcG b2778 0.88 -1.9 2,904,503 - ygcG b2778 0.88 +0.1 2,904,513 + ygcG b2778 0.89 +1.0 2,904,525 + -0.2 2,904,528 + -0.8 2,904,573 + -0.6 2,904,603 - -0.3 2,904,665 - -1.0
Or see this region's nucleotide sequence