Experiment: 3 hr rep1_Tiberius_10fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yodA and yodB are separated by 342 nucleotides yodB and serU are separated by 569 nucleotides serU and b1976 are separated by 93 nucleotides
b1973: yodA - conserved metal-binding protein (NCBI), at 2,039,399 to 2,040,049
yodA
b1974: yodB - putative cytochrome (VIMSS), at 2,040,392 to 2,040,922
yodB
b1975: serU - tRNA-Ser (NCBI), at 2,041,492 to 2,041,581
serU
b1976: b1976 - orf, hypothetical protein (VIMSS), at 2,041,675 to 2,042,472
b1976
Position (kb)
2040
2041 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2039.429 kb on + strand at 2039.439 kb on + strand at 2039.439 kb on + strand at 2039.451 kb on + strand at 2039.472 kb on + strand, within yodA at 2039.479 kb on + strand, within yodA at 2039.479 kb on + strand, within yodA at 2039.487 kb on - strand, within yodA at 2039.499 kb on - strand, within yodA at 2039.499 kb on - strand, within yodA at 2039.569 kb on + strand, within yodA at 2039.569 kb on - strand, within yodA at 2039.572 kb on + strand, within yodA at 2039.589 kb on + strand, within yodA at 2039.627 kb on + strand, within yodA at 2039.736 kb on + strand, within yodA at 2039.808 kb on + strand, within yodA at 2040.005 kb on - strand at 2040.010 kb on - strand at 2040.015 kb on - strand at 2040.023 kb on + strand at 2040.033 kb on - strand at 2040.095 kb on - strand at 2040.095 kb on - strand at 2040.139 kb on + strand at 2040.142 kb on + strand at 2040.214 kb on + strand at 2040.233 kb on + strand at 2040.249 kb on - strand at 2040.254 kb on - strand at 2040.254 kb on - strand at 2040.290 kb on - strand at 2040.363 kb on - strand at 2040.444 kb on - strand at 2040.462 kb on - strand, within yodB at 2040.492 kb on - strand, within yodB at 2040.493 kb on + strand, within yodB at 2040.494 kb on - strand at 2040.494 kb on - strand, within yodB at 2040.495 kb on - strand, within yodB at 2040.496 kb on - strand, within yodB at 2040.496 kb on - strand, within yodB at 2040.501 kb on + strand, within yodB at 2040.502 kb on - strand, within yodB at 2040.502 kb on - strand, within yodB at 2040.504 kb on - strand, within yodB at 2040.504 kb on - strand, within yodB at 2040.509 kb on - strand, within yodB at 2040.509 kb on - strand, within yodB at 2040.517 kb on - strand, within yodB at 2040.537 kb on - strand, within yodB at 2040.570 kb on - strand, within yodB at 2040.594 kb on - strand, within yodB at 2040.594 kb on - strand, within yodB at 2040.594 kb on - strand, within yodB at 2040.598 kb on + strand, within yodB at 2040.602 kb on - strand, within yodB at 2040.606 kb on - strand, within yodB at 2040.606 kb on - strand, within yodB at 2040.613 kb on + strand, within yodB at 2040.621 kb on - strand, within yodB at 2040.621 kb on - strand, within yodB at 2040.626 kb on + strand, within yodB at 2040.681 kb on + strand, within yodB at 2040.681 kb on + strand, within yodB at 2040.755 kb on + strand, within yodB at 2040.757 kb on + strand, within yodB at 2040.767 kb on - strand, within yodB at 2040.772 kb on - strand, within yodB at 2040.772 kb on - strand, within yodB at 2040.860 kb on - strand, within yodB at 2040.957 kb on + strand at 2040.957 kb on + strand at 2040.992 kb on + strand at 2041.038 kb on + strand at 2041.054 kb on - strand at 2041.236 kb on + strand at 2041.236 kb on + strand at 2041.359 kb on - strand at 2041.359 kb on - strand at 2041.395 kb on + strand at 2041.515 kb on - strand, within serU at 2041.871 kb on + strand, within b1976
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_10fold_dilution remove 2,039,429 + -1.8 2,039,439 + -1.5 2,039,439 + -1.3 2,039,451 + -1.0 2,039,472 + yodA b1973 0.11 -0.3 2,039,479 + yodA b1973 0.12 -0.7 2,039,479 + yodA b1973 0.12 +0.5 2,039,487 - yodA b1973 0.14 -1.1 2,039,499 - yodA b1973 0.15 -0.9 2,039,499 - yodA b1973 0.15 -1.1 2,039,569 + yodA b1973 0.26 +1.0 2,039,569 - yodA b1973 0.26 +1.3 2,039,572 + yodA b1973 0.27 -0.7 2,039,589 + yodA b1973 0.29 -0.5 2,039,627 + yodA b1973 0.35 +0.3 2,039,736 + yodA b1973 0.52 +1.3 2,039,808 + yodA b1973 0.63 +1.3 2,040,005 - +1.3 2,040,010 - +1.3 2,040,015 - +1.3 2,040,023 + +1.3 2,040,033 - +1.3 2,040,095 - -1.0 2,040,095 - +0.3 2,040,139 + +0.3 2,040,142 + +1.3 2,040,214 + +0.5 2,040,233 + +1.3 2,040,249 - -0.4 2,040,254 - +2.3 2,040,254 - +0.3 2,040,290 - -0.0 2,040,363 - +0.3 2,040,444 - -0.3 2,040,462 - yodB b1974 0.13 -0.1 2,040,492 - yodB b1974 0.19 -0.1 2,040,493 + yodB b1974 0.19 +1.3 2,040,494 - +1.3 2,040,494 - yodB b1974 0.19 -0.1 2,040,495 - yodB b1974 0.19 +0.4 2,040,496 - yodB b1974 0.20 -0.5 2,040,496 - yodB b1974 0.20 +0.2 2,040,501 + yodB b1974 0.21 +1.3 2,040,502 - yodB b1974 0.21 -1.3 2,040,502 - yodB b1974 0.21 -0.3 2,040,504 - yodB b1974 0.21 -1.0 2,040,504 - yodB b1974 0.21 -0.8 2,040,509 - yodB b1974 0.22 +0.2 2,040,509 - yodB b1974 0.22 -1.0 2,040,517 - yodB b1974 0.24 -0.7 2,040,537 - yodB b1974 0.27 -0.1 2,040,570 - yodB b1974 0.34 +0.7 2,040,594 - yodB b1974 0.38 -0.3 2,040,594 - yodB b1974 0.38 -1.3 2,040,594 - yodB b1974 0.38 -0.3 2,040,598 + yodB b1974 0.39 -0.0 2,040,602 - yodB b1974 0.40 +0.3 2,040,606 - yodB b1974 0.40 +0.2 2,040,606 - yodB b1974 0.40 -0.7 2,040,613 + yodB b1974 0.42 -0.3 2,040,621 - yodB b1974 0.43 +2.8 2,040,621 - yodB b1974 0.43 -1.6 2,040,626 + yodB b1974 0.44 -1.3 2,040,681 + yodB b1974 0.54 +1.3 2,040,681 + yodB b1974 0.54 -0.3 2,040,755 + yodB b1974 0.68 -0.5 2,040,757 + yodB b1974 0.69 -2.2 2,040,767 - yodB b1974 0.71 -0.8 2,040,772 - yodB b1974 0.72 -0.8 2,040,772 - yodB b1974 0.72 -1.0 2,040,860 - yodB b1974 0.88 -0.5 2,040,957 + -0.3 2,040,957 + +0.3 2,040,992 + +2.9 2,041,038 + +0.5 2,041,054 - -0.2 2,041,236 + -0.0 2,041,236 + -1.1 2,041,359 - +0.5 2,041,359 - +0.7 2,041,395 + -0.6 2,041,515 - serU b1975 0.26 -0.0 2,041,871 + b1976 b1976 0.25 +0.2
Or see this region's nucleotide sequence