Strain Fitness in Escherichia coli BW25113 around b1974

Experiment: 3 hr rep1_Tiberius_10fold_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyodA and yodB are separated by 342 nucleotidesyodB and serU are separated by 569 nucleotidesserU and b1976 are separated by 93 nucleotides b1973: yodA - conserved metal-binding protein (NCBI), at 2,039,399 to 2,040,049 yodA b1974: yodB - putative cytochrome (VIMSS), at 2,040,392 to 2,040,922 yodB b1975: serU - tRNA-Ser (NCBI), at 2,041,492 to 2,041,581 serU b1976: b1976 - orf, hypothetical protein (VIMSS), at 2,041,675 to 2,042,472 b1976 Position (kb) 2040 2041Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2039.429 kb on + strandat 2039.439 kb on + strandat 2039.439 kb on + strandat 2039.451 kb on + strandat 2039.472 kb on + strand, within yodAat 2039.479 kb on + strand, within yodAat 2039.479 kb on + strand, within yodAat 2039.487 kb on - strand, within yodAat 2039.499 kb on - strand, within yodAat 2039.499 kb on - strand, within yodAat 2039.569 kb on + strand, within yodAat 2039.569 kb on - strand, within yodAat 2039.572 kb on + strand, within yodAat 2039.589 kb on + strand, within yodAat 2039.627 kb on + strand, within yodAat 2039.736 kb on + strand, within yodAat 2039.808 kb on + strand, within yodAat 2040.005 kb on - strandat 2040.010 kb on - strandat 2040.015 kb on - strandat 2040.023 kb on + strandat 2040.033 kb on - strandat 2040.095 kb on - strandat 2040.095 kb on - strandat 2040.139 kb on + strandat 2040.142 kb on + strandat 2040.214 kb on + strandat 2040.233 kb on + strandat 2040.249 kb on - strandat 2040.254 kb on - strandat 2040.254 kb on - strandat 2040.290 kb on - strandat 2040.363 kb on - strandat 2040.444 kb on - strandat 2040.462 kb on - strand, within yodBat 2040.492 kb on - strand, within yodBat 2040.493 kb on + strand, within yodBat 2040.494 kb on - strandat 2040.494 kb on - strand, within yodBat 2040.495 kb on - strand, within yodBat 2040.496 kb on - strand, within yodBat 2040.496 kb on - strand, within yodBat 2040.501 kb on + strand, within yodBat 2040.502 kb on - strand, within yodBat 2040.502 kb on - strand, within yodBat 2040.504 kb on - strand, within yodBat 2040.504 kb on - strand, within yodBat 2040.509 kb on - strand, within yodBat 2040.509 kb on - strand, within yodBat 2040.517 kb on - strand, within yodBat 2040.537 kb on - strand, within yodBat 2040.570 kb on - strand, within yodBat 2040.594 kb on - strand, within yodBat 2040.594 kb on - strand, within yodBat 2040.594 kb on - strand, within yodBat 2040.598 kb on + strand, within yodBat 2040.602 kb on - strand, within yodBat 2040.606 kb on - strand, within yodBat 2040.606 kb on - strand, within yodBat 2040.613 kb on + strand, within yodBat 2040.621 kb on - strand, within yodBat 2040.621 kb on - strand, within yodBat 2040.626 kb on + strand, within yodBat 2040.681 kb on + strand, within yodBat 2040.681 kb on + strand, within yodBat 2040.755 kb on + strand, within yodBat 2040.757 kb on + strand, within yodBat 2040.767 kb on - strand, within yodBat 2040.772 kb on - strand, within yodBat 2040.772 kb on - strand, within yodBat 2040.860 kb on - strand, within yodBat 2040.957 kb on + strandat 2040.957 kb on + strandat 2040.992 kb on + strandat 2041.038 kb on + strandat 2041.054 kb on - strandat 2041.236 kb on + strandat 2041.236 kb on + strandat 2041.359 kb on - strandat 2041.359 kb on - strandat 2041.395 kb on + strandat 2041.515 kb on - strand, within serUat 2041.871 kb on + strand, within b1976

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Per-strain Table

Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_10fold_dilution
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2,039,429 + -1.8
2,039,439 + -1.5
2,039,439 + -1.3
2,039,451 + -1.0
2,039,472 + yodA b1973 0.11 -0.3
2,039,479 + yodA b1973 0.12 -0.7
2,039,479 + yodA b1973 0.12 +0.5
2,039,487 - yodA b1973 0.14 -1.1
2,039,499 - yodA b1973 0.15 -0.9
2,039,499 - yodA b1973 0.15 -1.1
2,039,569 + yodA b1973 0.26 +1.0
2,039,569 - yodA b1973 0.26 +1.3
2,039,572 + yodA b1973 0.27 -0.7
2,039,589 + yodA b1973 0.29 -0.5
2,039,627 + yodA b1973 0.35 +0.3
2,039,736 + yodA b1973 0.52 +1.3
2,039,808 + yodA b1973 0.63 +1.3
2,040,005 - +1.3
2,040,010 - +1.3
2,040,015 - +1.3
2,040,023 + +1.3
2,040,033 - +1.3
2,040,095 - -1.0
2,040,095 - +0.3
2,040,139 + +0.3
2,040,142 + +1.3
2,040,214 + +0.5
2,040,233 + +1.3
2,040,249 - -0.4
2,040,254 - +2.3
2,040,254 - +0.3
2,040,290 - -0.0
2,040,363 - +0.3
2,040,444 - -0.3
2,040,462 - yodB b1974 0.13 -0.1
2,040,492 - yodB b1974 0.19 -0.1
2,040,493 + yodB b1974 0.19 +1.3
2,040,494 - +1.3
2,040,494 - yodB b1974 0.19 -0.1
2,040,495 - yodB b1974 0.19 +0.4
2,040,496 - yodB b1974 0.20 -0.5
2,040,496 - yodB b1974 0.20 +0.2
2,040,501 + yodB b1974 0.21 +1.3
2,040,502 - yodB b1974 0.21 -1.3
2,040,502 - yodB b1974 0.21 -0.3
2,040,504 - yodB b1974 0.21 -1.0
2,040,504 - yodB b1974 0.21 -0.8
2,040,509 - yodB b1974 0.22 +0.2
2,040,509 - yodB b1974 0.22 -1.0
2,040,517 - yodB b1974 0.24 -0.7
2,040,537 - yodB b1974 0.27 -0.1
2,040,570 - yodB b1974 0.34 +0.7
2,040,594 - yodB b1974 0.38 -0.3
2,040,594 - yodB b1974 0.38 -1.3
2,040,594 - yodB b1974 0.38 -0.3
2,040,598 + yodB b1974 0.39 -0.0
2,040,602 - yodB b1974 0.40 +0.3
2,040,606 - yodB b1974 0.40 +0.2
2,040,606 - yodB b1974 0.40 -0.7
2,040,613 + yodB b1974 0.42 -0.3
2,040,621 - yodB b1974 0.43 +2.8
2,040,621 - yodB b1974 0.43 -1.6
2,040,626 + yodB b1974 0.44 -1.3
2,040,681 + yodB b1974 0.54 +1.3
2,040,681 + yodB b1974 0.54 -0.3
2,040,755 + yodB b1974 0.68 -0.5
2,040,757 + yodB b1974 0.69 -2.2
2,040,767 - yodB b1974 0.71 -0.8
2,040,772 - yodB b1974 0.72 -0.8
2,040,772 - yodB b1974 0.72 -1.0
2,040,860 - yodB b1974 0.88 -0.5
2,040,957 + -0.3
2,040,957 + +0.3
2,040,992 + +2.9
2,041,038 + +0.5
2,041,054 - -0.2
2,041,236 + -0.0
2,041,236 + -1.1
2,041,359 - +0.5
2,041,359 - +0.7
2,041,395 + -0.6
2,041,515 - serU b1975 0.26 -0.0
2,041,871 + b1976 b1976 0.25 +0.2

Or see this region's nucleotide sequence