Strain Fitness in Escherichia coli BW25113 around b0001

Experiment: 3 hr rep1_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrL and thrA are separated by 81 nucleotides b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255 thrL b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799 thrA Position (kb) 0 1Strain fitness (log2 ratio) -2 -1 0 1at 0.070 kb on + strandat 0.070 kb on + strandat 0.078 kb on - strandat 0.078 kb on - strandat 0.161 kb on - strandat 0.185 kb on - strandat 0.185 kb on - strandat 0.208 kb on + strand, within thrLat 0.217 kb on - strand, within thrLat 0.257 kb on - strandat 0.257 kb on - strandat 0.440 kb on - strandat 0.440 kb on - strandat 0.454 kb on - strandat 0.518 kb on - strandat 0.518 kb on - strandat 0.528 kb on + strandat 0.592 kb on + strand, within thrAat 0.592 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.675 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.767 kb on + strand, within thrAat 0.775 kb on - strand, within thrAat 0.957 kb on + strand, within thrAat 0.957 kb on + strand, within thrAat 1.046 kb on + strand, within thrAat 1.136 kb on - strand, within thrAat 1.142 kb on - strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrA

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Per-strain Table

Position Strand Gene LocusTag Fraction 3 hr rep1_Tiberius_no_dilution
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70 + -0.2
70 + +0.5
78 - -0.4
78 - +0.7
161 - +0.1
185 - -0.3
185 - -1.8
208 + thrL b0001 0.27 -0.5
217 - thrL b0001 0.41 -1.1
257 - -2.7
257 - +0.2
440 - +0.4
440 - -0.8
454 - +0.1
518 - -1.0
518 - +0.3
528 + +0.5
592 + thrA b0002 0.10 -0.4
592 + thrA b0002 0.10 +0.0
666 + thrA b0002 0.13 -0.1
666 + thrA b0002 0.13 -0.9
675 - thrA b0002 0.14 +0.2
754 - thrA b0002 0.17 -0.1
754 - thrA b0002 0.17 -0.8
767 + thrA b0002 0.17 -0.4
775 - thrA b0002 0.18 +0.2
957 + thrA b0002 0.25 -0.4
957 + thrA b0002 0.25 +0.6
1,046 + thrA b0002 0.29 -0.6
1,136 - thrA b0002 0.32 -0.0
1,142 - thrA b0002 0.33 -0.3
1,161 + thrA b0002 0.33 -1.5
1,161 + thrA b0002 0.33 +0.7
1,161 + thrA b0002 0.33 -0.1

Or see this region's nucleotide sequence