Experiment: 3 hr rep1_HD100_100fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sfmH and sfmF are separated by 10 nucleotides sfmF and fimZ are separated by 2 nucleotides fimZ and argU are separated by 242 nucleotides argU and intD are separated by 15 nucleotides
b0533: sfmH - predicted fimbrial-like adhesin protein (RefSeq), at 561,559 to 562,542
sfmH
b0534: sfmF - predicted fimbrial-like adhesin protein (NCBI), at 562,553 to 563,068
sfmF
b0535: fimZ - predicted DNA-binding transcriptional regulator (NCBI), at 563,071 to 563,703
fimZ
b0536: argU - tRNA-Arg (NCBI), at 563,946 to 564,022
argU
b0537: intD - DLP12 prophage; predicted integrase (NCBI), at 564,038 to 565,201
intD
Position (kb) 562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 561.623 kb on + strand at 561.634 kb on + strand at 561.634 kb on + strand at 561.680 kb on + strand, within sfmH at 561.726 kb on + strand, within sfmH at 561.726 kb on + strand, within sfmH at 561.726 kb on + strand, within sfmH at 561.745 kb on + strand, within sfmH at 561.745 kb on + strand, within sfmH at 561.747 kb on + strand, within sfmH at 561.749 kb on + strand, within sfmH at 561.761 kb on + strand, within sfmH at 561.761 kb on + strand, within sfmH at 561.920 kb on - strand, within sfmH at 562.008 kb on - strand, within sfmH at 562.008 kb on - strand, within sfmH at 562.008 kb on - strand, within sfmH at 562.018 kb on - strand, within sfmH at 562.027 kb on - strand, within sfmH at 562.027 kb on - strand, within sfmH at 562.027 kb on - strand, within sfmH at 562.027 kb on - strand, within sfmH at 562.027 kb on - strand, within sfmH at 562.028 kb on - strand, within sfmH at 562.037 kb on - strand, within sfmH at 562.037 kb on - strand, within sfmH at 562.038 kb on - strand, within sfmH at 562.038 kb on - strand, within sfmH at 562.042 kb on - strand, within sfmH at 562.044 kb on + strand, within sfmH at 562.050 kb on - strand, within sfmH at 562.054 kb on - strand, within sfmH at 562.054 kb on - strand, within sfmH at 562.058 kb on - strand, within sfmH at 562.060 kb on + strand, within sfmH at 562.060 kb on - strand, within sfmH at 562.060 kb on - strand, within sfmH at 562.067 kb on + strand, within sfmH at 562.067 kb on + strand, within sfmH at 562.118 kb on - strand, within sfmH at 562.191 kb on - strand, within sfmH at 562.191 kb on - strand, within sfmH at 562.191 kb on - strand, within sfmH at 562.191 kb on - strand, within sfmH at 562.191 kb on - strand, within sfmH at 562.217 kb on + strand, within sfmH at 562.222 kb on - strand, within sfmH at 562.223 kb on - strand, within sfmH at 562.227 kb on - strand, within sfmH at 562.228 kb on + strand, within sfmH at 562.231 kb on + strand, within sfmH at 562.303 kb on - strand, within sfmH at 562.312 kb on + strand, within sfmH at 562.312 kb on + strand, within sfmH at 562.331 kb on - strand, within sfmH at 562.331 kb on - strand, within sfmH at 562.334 kb on + strand, within sfmH at 562.386 kb on + strand, within sfmH at 562.445 kb on + strand at 562.445 kb on + strand at 562.457 kb on + strand at 562.533 kb on + strand at 562.533 kb on + strand at 562.552 kb on + strand at 562.570 kb on - strand at 562.594 kb on + strand at 562.666 kb on + strand, within sfmF at 562.666 kb on + strand, within sfmF at 562.666 kb on + strand, within sfmF at 562.716 kb on - strand, within sfmF at 562.716 kb on - strand, within sfmF at 562.720 kb on + strand, within sfmF at 562.738 kb on - strand, within sfmF at 562.824 kb on - strand, within sfmF at 562.834 kb on + strand, within sfmF at 562.839 kb on + strand, within sfmF at 562.858 kb on + strand, within sfmF at 562.892 kb on + strand, within sfmF at 562.897 kb on + strand, within sfmF at 562.905 kb on - strand, within sfmF at 562.911 kb on + strand, within sfmF at 562.911 kb on + strand, within sfmF at 562.912 kb on + strand, within sfmF at 562.917 kb on + strand, within sfmF at 562.917 kb on + strand, within sfmF at 562.917 kb on + strand, within sfmF at 562.917 kb on + strand, within sfmF at 562.922 kb on + strand, within sfmF at 562.922 kb on + strand, within sfmF at 562.927 kb on + strand, within sfmF at 562.927 kb on + strand, within sfmF at 562.931 kb on - strand, within sfmF at 562.931 kb on - strand, within sfmF at 562.931 kb on - strand, within sfmF at 562.963 kb on + strand, within sfmF at 563.005 kb on + strand, within sfmF at 563.005 kb on + strand, within sfmF at 563.005 kb on + strand, within sfmF at 563.005 kb on + strand, within sfmF at 563.005 kb on + strand, within sfmF at 563.013 kb on + strand, within sfmF at 563.013 kb on + strand, within sfmF at 563.013 kb on + strand, within sfmF at 563.015 kb on + strand, within sfmF at 563.024 kb on + strand at 563.024 kb on + strand at 563.024 kb on + strand at 563.024 kb on + strand at 563.024 kb on + strand at 563.058 kb on + strand at 563.257 kb on - strand, within fimZ at 563.355 kb on - strand, within fimZ at 563.413 kb on - strand, within fimZ at 563.423 kb on - strand, within fimZ at 563.427 kb on - strand, within fimZ at 563.438 kb on - strand, within fimZ at 563.482 kb on - strand, within fimZ at 563.482 kb on - strand, within fimZ at 563.482 kb on - strand, within fimZ at 563.489 kb on - strand, within fimZ at 563.513 kb on - strand, within fimZ at 563.522 kb on - strand, within fimZ at 563.527 kb on - strand, within fimZ at 563.542 kb on - strand, within fimZ at 563.542 kb on - strand, within fimZ at 563.561 kb on - strand, within fimZ at 563.610 kb on - strand, within fimZ at 563.620 kb on - strand, within fimZ at 563.675 kb on - strand at 563.683 kb on - strand at 563.684 kb on - strand at 563.684 kb on - strand at 563.684 kb on - strand at 563.684 kb on - strand at 563.684 kb on - strand at 563.684 kb on - strand at 563.686 kb on - strand at 563.705 kb on - strand at 563.794 kb on - strand at 563.802 kb on - strand at 563.809 kb on - strand at 563.828 kb on - strand at 563.860 kb on - strand at 563.925 kb on + strand at 563.925 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3 hr rep1_HD100_100fold_dilution remove 561,623 + -3.1 561,634 + -1.2 561,634 + -0.5 561,680 + sfmH b0533 0.12 +1.4 561,726 + sfmH b0533 0.17 -0.9 561,726 + sfmH b0533 0.17 -0.6 561,726 + sfmH b0533 0.17 +1.4 561,745 + sfmH b0533 0.19 +0.4 561,745 + sfmH b0533 0.19 +0.2 561,747 + sfmH b0533 0.19 -1.9 561,749 + sfmH b0533 0.19 -0.9 561,761 + sfmH b0533 0.21 -0.5 561,761 + sfmH b0533 0.21 +0.5 561,920 - sfmH b0533 0.37 +1.4 562,008 - sfmH b0533 0.46 -0.9 562,008 - sfmH b0533 0.46 -1.4 562,008 - sfmH b0533 0.46 -2.2 562,018 - sfmH b0533 0.47 -1.9 562,027 - sfmH b0533 0.48 +0.0 562,027 - sfmH b0533 0.48 -0.5 562,027 - sfmH b0533 0.48 +0.3 562,027 - sfmH b0533 0.48 -1.1 562,027 - sfmH b0533 0.48 -0.4 562,028 - sfmH b0533 0.48 -0.9 562,037 - sfmH b0533 0.49 +1.0 562,037 - sfmH b0533 0.49 +0.1 562,038 - sfmH b0533 0.49 -0.5 562,038 - sfmH b0533 0.49 -0.4 562,042 - sfmH b0533 0.49 +0.3 562,044 + sfmH b0533 0.49 +1.4 562,050 - sfmH b0533 0.50 -2.6 562,054 - sfmH b0533 0.50 +0.6 562,054 - sfmH b0533 0.50 -1.9 562,058 - sfmH b0533 0.51 -0.8 562,060 + sfmH b0533 0.51 -1.6 562,060 - sfmH b0533 0.51 +1.8 562,060 - sfmH b0533 0.51 +0.1 562,067 + sfmH b0533 0.52 +0.4 562,067 + sfmH b0533 0.52 -1.8 562,118 - sfmH b0533 0.57 -0.9 562,191 - sfmH b0533 0.64 -0.1 562,191 - sfmH b0533 0.64 +0.8 562,191 - sfmH b0533 0.64 -0.2 562,191 - sfmH b0533 0.64 -0.1 562,191 - sfmH b0533 0.64 -2.7 562,217 + sfmH b0533 0.67 +1.4 562,222 - sfmH b0533 0.67 -0.4 562,223 - sfmH b0533 0.67 -1.5 562,227 - sfmH b0533 0.68 -0.5 562,228 + sfmH b0533 0.68 -2.8 562,231 + sfmH b0533 0.68 -0.2 562,303 - sfmH b0533 0.76 +0.7 562,312 + sfmH b0533 0.77 -1.4 562,312 + sfmH b0533 0.77 -2.1 562,331 - sfmH b0533 0.78 +1.4 562,331 - sfmH b0533 0.78 +0.6 562,334 + sfmH b0533 0.79 +2.2 562,386 + sfmH b0533 0.84 +0.0 562,445 + +0.6 562,445 + +0.5 562,457 + +0.2 562,533 + +0.2 562,533 + +0.1 562,552 + -0.1 562,570 - +0.9 562,594 + -0.2 562,666 + sfmF b0534 0.22 +0.9 562,666 + sfmF b0534 0.22 +0.8 562,666 + sfmF b0534 0.22 -0.6 562,716 - sfmF b0534 0.32 -1.2 562,716 - sfmF b0534 0.32 -0.2 562,720 + sfmF b0534 0.32 +0.3 562,738 - sfmF b0534 0.36 -1.4 562,824 - sfmF b0534 0.53 -2.0 562,834 + sfmF b0534 0.54 +0.6 562,839 + sfmF b0534 0.55 -0.5 562,858 + sfmF b0534 0.59 -0.2 562,892 + sfmF b0534 0.66 -0.9 562,897 + sfmF b0534 0.67 -0.4 562,905 - sfmF b0534 0.68 +1.4 562,911 + sfmF b0534 0.69 +0.1 562,911 + sfmF b0534 0.69 +0.2 562,912 + sfmF b0534 0.70 +0.2 562,917 + sfmF b0534 0.71 +1.0 562,917 + sfmF b0534 0.71 +0.5 562,917 + sfmF b0534 0.71 +0.0 562,917 + sfmF b0534 0.71 -0.3 562,922 + sfmF b0534 0.72 -0.3 562,922 + sfmF b0534 0.72 -0.7 562,927 + sfmF b0534 0.72 +0.8 562,927 + sfmF b0534 0.72 +1.4 562,931 - sfmF b0534 0.73 +0.8 562,931 - sfmF b0534 0.73 +1.4 562,931 - sfmF b0534 0.73 +1.4 562,963 + sfmF b0534 0.79 -0.5 563,005 + sfmF b0534 0.88 -0.4 563,005 + sfmF b0534 0.88 -1.6 563,005 + sfmF b0534 0.88 +0.7 563,005 + sfmF b0534 0.88 -1.6 563,005 + sfmF b0534 0.88 -0.9 563,013 + sfmF b0534 0.89 +0.1 563,013 + sfmF b0534 0.89 +0.5 563,013 + sfmF b0534 0.89 -0.7 563,015 + sfmF b0534 0.90 -0.5 563,024 + -2.5 563,024 + -1.0 563,024 + -0.7 563,024 + +0.2 563,024 + -0.3 563,058 + +1.4 563,257 - fimZ b0535 0.29 +1.4 563,355 - fimZ b0535 0.45 -2.3 563,413 - fimZ b0535 0.54 -1.5 563,423 - fimZ b0535 0.56 -1.6 563,427 - fimZ b0535 0.56 -0.1 563,438 - fimZ b0535 0.58 +1.4 563,482 - fimZ b0535 0.65 +1.7 563,482 - fimZ b0535 0.65 -1.2 563,482 - fimZ b0535 0.65 -1.6 563,489 - fimZ b0535 0.66 -0.6 563,513 - fimZ b0535 0.70 +0.0 563,522 - fimZ b0535 0.71 +0.4 563,527 - fimZ b0535 0.72 +0.9 563,542 - fimZ b0535 0.74 -0.6 563,542 - fimZ b0535 0.74 -0.1 563,561 - fimZ b0535 0.77 -0.2 563,610 - fimZ b0535 0.85 +0.0 563,620 - fimZ b0535 0.87 -2.4 563,675 - -0.6 563,683 - +0.0 563,684 - -1.6 563,684 - -0.2 563,684 - -0.2 563,684 - -1.0 563,684 - -1.6 563,684 - +1.4 563,686 - -0.7 563,705 - -0.1 563,794 - -0.2 563,802 - +0.1 563,809 - -0.9 563,828 - +1.0 563,860 - +0.4 563,925 + -0.7 563,925 + +0.2
Or see this region's nucleotide sequence