Strain Fitness in Escherichia coli BW25113 around b2132

Experiment: 48 hr rep3_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehZ and bglX are separated by 210 nucleotidesbglX and dld are separated by 195 nucleotides b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503 yehZ b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011 bglX b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922 dld Position (kb) 2217 2218 2219 2220 2221Strain fitness (log2 ratio) -3 -2 -1 0 1at 2216.745 kb on - strand, within yehZat 2216.745 kb on - strand, within yehZat 2216.748 kb on - strand, within yehZat 2216.767 kb on - strand, within yehZat 2216.782 kb on + strand, within yehZat 2216.950 kb on - strand, within yehZat 2216.950 kb on - strand, within yehZat 2216.955 kb on + strand, within yehZat 2216.955 kb on + strand, within yehZat 2217.019 kb on + strand, within yehZat 2217.153 kb on - strand, within yehZat 2217.153 kb on - strand, within yehZat 2217.260 kb on + strand, within yehZat 2217.311 kb on - strand, within yehZat 2217.311 kb on - strand, within yehZat 2217.328 kb on + strand, within yehZat 2217.376 kb on + strand, within yehZat 2217.463 kb on + strandat 2217.470 kb on + strandat 2217.560 kb on - strandat 2217.621 kb on - strandat 2217.682 kb on - strandat 2217.769 kb on + strandat 2218.066 kb on + strand, within bglXat 2218.089 kb on + strand, within bglXat 2218.089 kb on + strand, within bglXat 2218.305 kb on - strand, within bglXat 2218.470 kb on + strand, within bglXat 2218.470 kb on + strand, within bglXat 2218.476 kb on - strand, within bglXat 2218.476 kb on - strand, within bglXat 2218.483 kb on - strand, within bglXat 2218.483 kb on - strand, within bglXat 2218.544 kb on + strand, within bglXat 2218.544 kb on + strand, within bglXat 2218.688 kb on - strand, within bglXat 2218.688 kb on - strand, within bglXat 2218.767 kb on - strand, within bglXat 2218.871 kb on - strand, within bglXat 2218.871 kb on - strand, within bglXat 2218.974 kb on - strand, within bglXat 2219.011 kb on + strand, within bglXat 2219.011 kb on + strand, within bglXat 2219.038 kb on + strand, within bglXat 2219.038 kb on + strand, within bglXat 2219.043 kb on + strand, within bglXat 2219.130 kb on - strand, within bglXat 2219.130 kb on - strand, within bglXat 2219.140 kb on - strand, within bglXat 2219.307 kb on - strand, within bglXat 2219.343 kb on + strand, within bglXat 2219.398 kb on + strand, within bglXat 2219.398 kb on + strand, within bglXat 2219.407 kb on + strand, within bglXat 2219.415 kb on - strand, within bglXat 2219.539 kb on - strand, within bglXat 2219.559 kb on - strand, within bglXat 2219.659 kb on - strand, within bglXat 2219.664 kb on - strand, within bglXat 2219.891 kb on + strandat 2219.891 kb on + strandat 2219.969 kb on + strandat 2219.969 kb on + strandat 2219.977 kb on - strandat 2219.978 kb on - strandat 2219.998 kb on - strandat 2220.036 kb on - strandat 2220.147 kb on + strandat 2220.147 kb on + strandat 2220.167 kb on - strandat 2220.167 kb on - strandat 2220.206 kb on - strandat 2220.206 kb on - strandat 2220.220 kb on - strandat 2220.276 kb on - strandat 2220.276 kb on - strandat 2220.279 kb on - strandat 2220.330 kb on + strandat 2220.560 kb on - strand, within dldat 2220.623 kb on + strand, within dldat 2220.635 kb on + strand, within dldat 2220.651 kb on - strand, within dldat 2220.781 kb on + strand, within dldat 2220.781 kb on + strand, within dldat 2220.784 kb on - strand, within dldat 2220.784 kb on - strand, within dldat 2220.811 kb on + strand, within dld

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Per-strain Table

Position Strand Gene LocusTag Fraction 48 hr rep3_Tiberius_no_dilution
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2,216,745 - yehZ b2131 0.17 -1.7
2,216,745 - yehZ b2131 0.17 -0.6
2,216,748 - yehZ b2131 0.18 +0.7
2,216,767 - yehZ b2131 0.20 -0.5
2,216,782 + yehZ b2131 0.21 +0.5
2,216,950 - yehZ b2131 0.40 -1.2
2,216,950 - yehZ b2131 0.40 -0.5
2,216,955 + yehZ b2131 0.40 +1.4
2,216,955 + yehZ b2131 0.40 +0.2
2,217,019 + yehZ b2131 0.47 -0.9
2,217,153 - yehZ b2131 0.62 +0.7
2,217,153 - yehZ b2131 0.62 -0.0
2,217,260 + yehZ b2131 0.73 -0.2
2,217,311 - yehZ b2131 0.79 +0.2
2,217,311 - yehZ b2131 0.79 -0.0
2,217,328 + yehZ b2131 0.81 +0.2
2,217,376 + yehZ b2131 0.86 -0.0
2,217,463 + +0.8
2,217,470 + -0.1
2,217,560 - +0.7
2,217,621 - -0.0
2,217,682 - +0.6
2,217,769 + -0.5
2,218,066 + bglX b2132 0.15 -1.3
2,218,089 + bglX b2132 0.16 -1.0
2,218,089 + bglX b2132 0.16 -0.6
2,218,305 - bglX b2132 0.26 +0.8
2,218,470 + bglX b2132 0.33 -0.3
2,218,470 + bglX b2132 0.33 -0.5
2,218,476 - bglX b2132 0.33 +0.2
2,218,476 - bglX b2132 0.33 +0.1
2,218,483 - bglX b2132 0.33 -0.1
2,218,483 - bglX b2132 0.33 -0.1
2,218,544 + bglX b2132 0.36 +0.3
2,218,544 + bglX b2132 0.36 -0.1
2,218,688 - bglX b2132 0.42 -1.6
2,218,688 - bglX b2132 0.42 -0.4
2,218,767 - bglX b2132 0.46 -0.2
2,218,871 - bglX b2132 0.50 +0.7
2,218,871 - bglX b2132 0.50 +0.5
2,218,974 - bglX b2132 0.55 +0.1
2,219,011 + bglX b2132 0.56 +0.6
2,219,011 + bglX b2132 0.56 -0.1
2,219,038 + bglX b2132 0.58 -1.6
2,219,038 + bglX b2132 0.58 -0.2
2,219,043 + bglX b2132 0.58 -0.0
2,219,130 - bglX b2132 0.62 -0.6
2,219,130 - bglX b2132 0.62 +0.4
2,219,140 - bglX b2132 0.62 -0.3
2,219,307 - bglX b2132 0.69 -1.1
2,219,343 + bglX b2132 0.71 -1.9
2,219,398 + bglX b2132 0.73 +0.2
2,219,398 + bglX b2132 0.73 +0.7
2,219,407 + bglX b2132 0.74 +0.1
2,219,415 - bglX b2132 0.74 -1.3
2,219,539 - bglX b2132 0.79 +0.1
2,219,559 - bglX b2132 0.80 +0.0
2,219,659 - bglX b2132 0.85 -0.5
2,219,664 - bglX b2132 0.85 +0.1
2,219,891 + +0.3
2,219,891 + +0.3
2,219,969 + -3.5
2,219,969 + +0.0
2,219,977 - -0.4
2,219,978 - -0.0
2,219,998 - +0.5
2,220,036 - -0.1
2,220,147 + -2.3
2,220,147 + +0.1
2,220,167 - -0.8
2,220,167 - +0.9
2,220,206 - -0.5
2,220,206 - +0.4
2,220,220 - +0.1
2,220,276 - +0.1
2,220,276 - +0.0
2,220,279 - -0.4
2,220,330 + -0.4
2,220,560 - dld b2133 0.21 +0.0
2,220,623 + dld b2133 0.24 +0.2
2,220,635 + dld b2133 0.25 -0.3
2,220,651 - dld b2133 0.26 -0.4
2,220,781 + dld b2133 0.33 -0.8
2,220,781 + dld b2133 0.33 -1.0
2,220,784 - dld b2133 0.34 +0.6
2,220,784 - dld b2133 0.34 -0.6
2,220,811 + dld b2133 0.35 -0.3

Or see this region's nucleotide sequence