Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppB and oppC are separated by 14 nucleotides oppC and oppD are separated by 11 nucleotides oppD and oppF overlap by 4 nucleotides
b1244: oppB - oligopeptide permease ABC transporter membrane protein (NCBI), at 1,300,923 to 1,301,843
oppB
b1245: oppC - oligopeptide transporter subunit (NCBI), at 1,301,858 to 1,302,766
oppC
b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791
oppD
b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792
oppF
Position (kb)
1302
1303
1304 Strain fitness (log2 ratio)
-1
0
1
2 at 1301.921 kb on - strand at 1301.964 kb on + strand, within oppC at 1301.982 kb on + strand, within oppC at 1302.053 kb on + strand, within oppC at 1302.057 kb on - strand, within oppC at 1302.115 kb on + strand, within oppC at 1302.115 kb on + strand, within oppC at 1302.159 kb on + strand, within oppC at 1302.379 kb on - strand, within oppC at 1302.506 kb on + strand, within oppC at 1302.506 kb on + strand, within oppC at 1302.562 kb on + strand, within oppC at 1302.650 kb on + strand, within oppC at 1302.650 kb on + strand, within oppC at 1302.671 kb on + strand, within oppC at 1302.762 kb on + strand at 1302.762 kb on + strand at 1302.770 kb on + strand at 1302.770 kb on + strand at 1302.770 kb on + strand at 1302.812 kb on + strand at 1302.812 kb on + strand at 1302.860 kb on + strand at 1302.933 kb on + strand, within oppD at 1303.045 kb on + strand, within oppD at 1303.071 kb on + strand, within oppD at 1303.132 kb on + strand, within oppD at 1303.142 kb on + strand, within oppD at 1303.155 kb on + strand, within oppD at 1303.165 kb on + strand, within oppD at 1303.201 kb on - strand, within oppD at 1303.342 kb on - strand, within oppD at 1303.359 kb on + strand, within oppD at 1303.403 kb on - strand, within oppD at 1303.405 kb on + strand, within oppD at 1303.434 kb on - strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.517 kb on + strand, within oppD at 1303.563 kb on + strand, within oppD at 1303.571 kb on - strand, within oppD at 1303.902 kb on + strand, within oppF at 1303.914 kb on - strand, within oppF at 1303.981 kb on + strand, within oppF at 1303.981 kb on + strand, within oppF at 1304.158 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.367 kb on + strand, within oppF at 1304.720 kb on + strand at 1304.780 kb on - strand at 1304.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,301,921 - +0.1 1,301,964 + oppC b1245 0.12 +0.4 1,301,982 + oppC b1245 0.14 +0.7 1,302,053 + oppC b1245 0.21 +0.0 1,302,057 - oppC b1245 0.22 +0.8 1,302,115 + oppC b1245 0.28 -0.1 1,302,115 + oppC b1245 0.28 -0.1 1,302,159 + oppC b1245 0.33 +0.3 1,302,379 - oppC b1245 0.57 +2.1 1,302,506 + oppC b1245 0.71 +1.0 1,302,506 + oppC b1245 0.71 -0.7 1,302,562 + oppC b1245 0.77 +0.3 1,302,650 + oppC b1245 0.87 -0.1 1,302,650 + oppC b1245 0.87 +0.1 1,302,671 + oppC b1245 0.89 -0.3 1,302,762 + -0.1 1,302,762 + -0.8 1,302,770 + -0.1 1,302,770 + +0.8 1,302,770 + +0.6 1,302,812 + +0.6 1,302,812 + +0.4 1,302,860 + -1.0 1,302,933 + oppD b1246 0.15 +0.3 1,303,045 + oppD b1246 0.26 -0.1 1,303,071 + oppD b1246 0.29 +0.3 1,303,132 + oppD b1246 0.35 +0.8 1,303,142 + oppD b1246 0.36 +0.3 1,303,155 + oppD b1246 0.37 -0.1 1,303,165 + oppD b1246 0.38 +2.2 1,303,201 - oppD b1246 0.42 +0.7 1,303,342 - oppD b1246 0.56 +0.4 1,303,359 + oppD b1246 0.57 +0.9 1,303,403 - oppD b1246 0.62 -1.2 1,303,405 + oppD b1246 0.62 -0.5 1,303,434 - oppD b1246 0.65 -0.4 1,303,439 + oppD b1246 0.65 +0.2 1,303,439 + oppD b1246 0.65 +1.2 1,303,517 + oppD b1246 0.73 +0.5 1,303,563 + oppD b1246 0.77 +0.4 1,303,571 - oppD b1246 0.78 +1.0 1,303,902 + oppF b1247 0.11 -0.2 1,303,914 - oppF b1247 0.13 +0.1 1,303,981 + oppF b1247 0.19 +0.2 1,303,981 + oppF b1247 0.19 -0.1 1,304,158 + oppF b1247 0.37 +0.4 1,304,294 + oppF b1247 0.50 -0.8 1,304,294 + oppF b1247 0.50 +0.8 1,304,367 + oppF b1247 0.58 +1.8 1,304,720 + +0.7 1,304,780 - -0.8 1,304,780 - +0.9
Or see this region's nucleotide sequence